Characterization and Multipotentiality of Human Fetal Femur–Derived Cells: Implications for Skeletal Tissue Regeneration

Mirmalek-Sani, Sayed Hadi; Tare, Rahul S.; Morgan, Sara; Roach, Helmtrud I.; Wilson, David I.; Hanley, Neil A.; Oreffo, Richard O.C.

doi:10.1634/stemcells.2005-0368

Cited by 89 publications

(87 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is most probable that this elongated morphology causes cellular cytoskeletal tension and stress on the hMSCs cultured on 70-to 100-nm diameter regime nanotubes compared with a normal cell culture condition on a flat surface substrate. It is well known that various kinds of physical stresses from the substrate morphology and topography can accelerate stem cell differentiation into a specific cell lineage (29)(30)(31)(32)(33)(34)(35); our data show a similar scenario.…”

Section: Discussionsupporting

confidence: 77%

Stem cell fate dictated solely by altered nanotube dimension

Oh¹,

Brammer²,

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

1,088

909

View full text Add to dashboard Cite

Two important goals in stem cell research are to control the cell proliferation without differentiation and to direct the differentiation into a specific cell lineage when desired. Here, we demonstrate such paths by controlling only the nanotopography of culture substrates. Altering the dimensions of nanotubular-shaped titanium oxide surface structures independently allowed either augmented human mesenchymal stem cell (hMSC) adhesion or a specific differentiation of hMSCs into osteoblasts by using only the geometric cues, absent of osteogenic inducing media. hMSC behavior in response to defined nanotube sizes revealed a very dramatic change in hMSC behavior in a relatively narrow range of nanotube dimensions. Small (Ϸ30-nm diameter) nanotubes promoted adhesion without noticeable differentiation, whereas larger (Ϸ70-to 100-nm diameter) nanotubes elicited a dramatic stem cell elongation (Ϸ10-fold increased), which induced cytoskeletal stress and selective differentiation into osteoblast-like cells, offering a promising nanotechnology-based route for unique orthopedics-related hMSC treatments.differentiation ͉ mesenchymal ͉ nanotopography ͉ osteogenesis ͉ proliferation N anostructures are of particular interest because they have the advantageous feature of a high surface-to-volume ratio, and they elicit a higher degree of biological plasticity compared with conventional micro-or macrostructures. In the field of biomaterial development and in vivo implant technology, the nanoscale structure and morphologenic factor of the surface have played a critical role in accelerating the rate of cell proliferation and enhancing tissue acceptance with a reduced immune response (1, 2). In terms of in vitro cell biology, there has also been much attention placed on cellular responses to their structural surroundings (3). In fact, it has been observed that macro-, micro-and nano-sized topographical factors stimulate behavioral changes in both cells and tissues. Recent studies related to the effect of nanotopography on cellular behavior indicated that osteoblast adhesion and functionality was enhanced by 30% when cultured on a nanograined Al 2 O 3 and TiO 2 substrate (4-6) compared with those cultured on a micrograined surface, and nanostructures such as TiO 2 nanotubes with Ͻ100-nm spacing showed superior characteristics in bone mineral synthesis (5). However, most of the previous studies on nanostructures and cell responses have mainly used oriented, patterned, or semiordered polymer arrays (7-9) and alumina/ polymer hybrid patterned arrays (10).The material and mechanical characteristics of titanium (Ti) metal, which has a thin native oxide layer of TiO 2 , make it an ideal orthopedic material that bonds directly to the adjacent bone surface (11,12). Fabrication of the nanostructured titanium dioxide (TiO 2 ) nanotube arrays has been a primary subject of investigation lately because of the wide range of TiO 2 applications in the fields of solar cells (13-16), photocatalysis (17-19), photoelectrolysis (20), sensors (21,22), and b...

show abstract

Section: Discussionsupporting

confidence: 77%

Stem cell fate dictated solely by altered nanotube dimension

Oh¹,

Brammer²,

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

1,088

909

View full text Add to dashboard Cite

show abstract

“…Mesenchymal stem cells can give rise to cells of the adipogenic, reticular, osteoblastic, myoblastic, and fibroblastic lineages. Due to the developmental plasticity of mesenchymal stem cells there is tremendous interest in their application to replace damaged tissues (Oreffo et al 2005;Mirmalek-Sani et al 2006). These progenitor populations offer compelling strategies to replace or restore the function of traumatized, diseased, or lost tissue.…”

Section: Introductionmentioning

confidence: 99%

Adipocyte differentiation induced using nonspecific siRNA controls in cultured human mesenchymal stem cells

Xu¹,

Mirmalek-Sani²,

Feng³

et al. 2007

RNA

Self Cite

View full text Add to dashboard Cite

RNA interference (RNAi) is gene silencing induced by double-stranded RNA of 21-23 nucleotides in length, termed small interfering RNA, or siRNA. RNAi-based techniques have been widely applied to elucidate gene function, identify drug targets, and used in trials as a promising adjunct to silence disease-causing genes. However, emerging evidence suggests unexpected changes in expression of untargeted genes as a consequence of an off-target effect by RNAi in mammalian cells. To date, our understanding of such effects on stem cells is limited. We transfected human fetal femur-derived mesenchymal stem cells using commercially available nonspecific siRNA controls and examined adipocyte differentiation in the cells using morphology, histochemistry, and quantitative real-time PCR to examine the expression of key genes for adipogenic or osteogenic differentiation. We report here the induction of adipocyte differentiation in human mesenchymal stem cells using nonspecific siRNAs raising concerns as to the specificity of RNAi in stem cells and, critically, a need to understand and delineate the rules governing the specificity of RNAi.

show abstract

“…Cells were seeded at high confluence (80-90%) prior to differentiation. Adipogenic differentiation was induced using induction media constituting 1 µm dexamethasone, 1.7 µm insulin, 200 µm indomethacin, and 500 µm isobutylmethylxanthine [18,29] in DMEM. This was alternated with DMEM containing 1.7 µm insulin [18a,29b] as a maintenance media at regular intervals.…”

Section: Methodsmentioning

confidence: 99%

The Prismatic Topography of Pinctada maxima Shell Retains Stem Cell Multipotency and Plasticity In Vitro

et al. 2018

View full text Add to dashboard Cite

cell migration, [2] differentiation, [3] and maintaining the self-renewal property of stem cells. [3b,4] as such, these characteristics play an important role in the design and development of biocompatible materials able to direct and sustain any number of cellular functions for tissue engineering applications.Biomineralization is the process by which organisms sequester simple inorganic components to create hardened tissue types. In nature, these structures accomplish a range of functions including photoreception, [5] gravity sensing and balance, [6] defense against predation in invertebrate shells, and support in vertebrate skeletons. In vertebrates, calcium phosphate is used to form bones and teeth. Invertebrate systems such as corals and shells utilize calcium carbonate which can be assembled in a number of ways generating a staggering amount of structural diversity. [7] A small (5%) component of the shell is organic matrix which drives and orchestrates the biomineralization process. [8] These are responsible for the assembly and mineralization of each of the calcium carbonate polymorphs such as calcite and aragonite, resulting in their very distinct overall composite structures. [9] This "phosphate-carbonate break" in genealogical similarityThe shell of the bivalve mollusc Pinctada maxima is composed of the calcium carbonate polymorphs calcite and aragonite (nacre). Mother-of-pearl, or nacre, induces vertebrate cells to undergo osteogenesis and has good osteointegrative qualities in vivo. The calcite counterpart, however, is less researched in terms of the response of vertebrate cells. This study shows that isolation of calcite surface topography from the inherent chemistry allows viable long-term culture of bone marrow derived mesenchymal stem cells (MSCs). Self-renewal is evident from the increased gene expression of the selfrenewal markers CD63, CD166, and CD271 indicating that cells cultured on the calcite topography maintain their stem cell phenotype. MSCs also retain their multipotency and can undergo successful differentiation into osteoblasts and adipocytes. When directed to adipogenesis, MSCs cultured on prism replicas are more amenable to differentiation than MSCs cultured on tissue culture polystyrene indicating a higher degree of plasticity in MSCs growing on calcite P. maxima prismatic topography. The study highlights the potential of the calcite topography of P. maxima as a biomimetic design for supporting expansion of MSC populations in vitro, which is of fundamental importance if it meets the demands for autologous MSCs for therapeutic use.Stem Cells

show abstract

Characterization and Multipotentiality of Human Fetal Femur–Derived Cells: Implications for Skeletal Tissue Regeneration

Cited by 89 publications

References 64 publications

Stem cell fate dictated solely by altered nanotube dimension

Stem cell fate dictated solely by altered nanotube dimension

Adipocyte differentiation induced using nonspecific siRNA controls in cultured human mesenchymal stem cells

The Prismatic Topography of Pinctada maxima Shell Retains Stem Cell Multipotency and Plasticity In Vitro

Contact Info

Product

Resources

About