Characterization and mosquitocidal potency of a Bacillus thuringiensis strain of rice field soil of Burdwan, West Bengal, India

Roy, Milita; Chatterjee, Soumendranath; Dangar, Tushar Kanti

doi:10.1016/j.micpath.2021.105093

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…quinquefasciatus and An. stephensi (Roy et al, 2021;Alves et al, 2023). Bacillus cereus isolated from fresh water fish Clarias batrachus also showed promising activity against the larvae of Ae.…”

Section: Resultsmentioning

confidence: 99%

Isolation of Mosquitocidal Bacteria from Soil Samples to Control Mosquito Vectors

Gangmei,

Padmanaban,

Kunnikuruvan

et al. 2024

IJE

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In the present study, an attempt was made to screen and isolate potent and ecofriendly mosquitocidal bacteria from different soils collected from Union Territory of Puducherry, India. From a total of 140 soil samples, 16 isolates showed mosquitocidal activity. Out of these 16 isolates, two bacteria isolated from red soil of Kalapet and Kanagachettikulam villages of Union Territory of Puducherry, India with code no. PYKAL-31A and PYKC-33C were found to be most potent. Extensive bioassay was carried out with these two bacterial strains against larvae of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. The LC50 and LC90 values of PYKAL-31A against Aedes aegypti, Culex quinquefasciatus and Anopheles stephensi were 0.007, 0.011, 0.015 and 0.015, 0.021 and 0.029, respectively and that of PYKC-33C were 0.009, 0.014, 0.013 and 0.015, 0.026, 0.023, respectively.

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Section: Resultsmentioning

confidence: 99%

Isolation of Mosquitocidal Bacteria from Soil Samples to Control Mosquito Vectors

Gangmei,

Padmanaban,

Kunnikuruvan

et al. 2024

IJE

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“…Moreover, this bacterium has a wide spectrum of inhibitory properties, rapid reproduction, low production costs, a good safety profile, and a wide variety of advantageous properties for biological control. The main species of Bacillus used in biological control research are Bacillus subtilis , Bacillus thuringiensis , Bacillus amyloliq uefaciens , and Bacillus polymyxa ( Wang et al, 2019 ; Roy et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Antifungal mechanisms of the antagonistic bacterium Bacillus mojavensis UTF-33 and its potential as a new biopesticide

Zhang¹,

Yang²,

Zhang³

et al. 2023

Front. Microbiol.

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Biological control has gradually become the dominant means of controlling fungal disease over recent years. In this study, an endophytic strain of UTF-33 was isolated from acid mold (Rumex acetosa L.) leaves. Based on 16S rDNA gene sequence comparison, and biochemical and physiological characteristics, this strain was formally identified as Bacillus mojavensis. Bacillus mojavensis UTF-33 was sensitive to most of the antibiotics tested except neomycin. Moreover, the filtrate fermentation solution of Bacillus mojavensis UTF-33 had a significant inhibitory effect on the growth of rice blast and was used in field evaluation tests, which reduced the infestation of rice blast effectively. Rice treated with filtrate fermentation broth exhibited multiple defense mechanisms in response, including the enhanced expression of disease process-related genes and transcription factor genes, and significantly upregulated the gene expression of titin, salicylic acid pathway-related genes, and H2O2 accumulation, in plants; this may directly or indirectly act as an antagonist to pathogenic infestation. Further analysis revealed that the n-butanol crude extract of Bacillus mojavensis UTF-33 could retard or even inhibit conidial germination and prevent the formation of adherent cells both in vitro and in vivo. In addition, the amplification of functional genes for biocontrol using specific primers showed that Bacillus mojavensis UTF-33 expresses genes that can direct the synthesis of bioA, bmyB, fenB, ituD, srfAA and other substances; this information can help us to determine the extraction direction and purification method for inhibitory substances at a later stage. In conclusion, this is the first study to identify Bacillus mojavensis as a potential agent for the control of rice diseases; this strain, and its bioactive substances, have the potential to be developed as biopesticides.

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Characterization of entomotoxic and nematotoxic genes from indigenous Bacillus thuringiensis strains and their biocontrol potential

Sujayanand

Pandey

Jagadeeswaran

et al. 2023

Egypt J Biol Pest Control

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Background The gram pod borer (Helicoverpa armigera Hubner), Bihar hairy caterpillar (Spilosoma obliqua Walker), tobacco caterpillar (Spodoptera litura Fabricius) and root knot nematode (Meloidogyne incognita (Kofoid and White) Chitwood) are the major insect pest and nematodes infesting mungbean, urdbean and other legumes. Bacillus thuringiensis (Bt) is a promising biocontrol agent that helps in managing the above pest. Deploying Bt biopesticides instead of chemical pesticides in legume cultivation enhances the soil health substantially. The present study envisages morphological, molecular cum biochemical characterization, entomotoxicity and nematotoxicity potential of four indigenous Bt strains (Ak2.IIPR, F8.IIPR, F5.IIPR and F6.IIPR) along with HD1 (standard check from BGSC). Results The SEM micrograph analysis of above four Bt strains along with F1.IIPR revealed the presence of different combinations of insecticidal crystal proteins, viz. cuboidal crystal (CC), bipyramidal crystal (BC), spherical crystal (SC) and irregularly pointed crystal (IC). This was further confirmed by PCR amplification of Cry1, Cry2Aa, Cry2Ac, VIP3A and chi36 gene-specific primers and their phylogenetic analysis. F8.IIPR and Ak2.IIPR recorded the highest toxicity index against second-instar S. obliqua larvae (292.56 and 174.55), H. armigera (150.00 and 113.65) and S. litura (210.71 and 114.19) based on probit analysis. F5.IIPR (91.67%) and F8.IIPR (89.00%) recorded the highest juvenile mortality against M. incognita, followed by Ak2.IIPR (70.33%). The protein profiling of supernatant of terrific broth inoculated with F8.IIPR indicated the presence of VIP3A and Cry1I. Conclusion The present study concluded that F8.IIPR and Ak2.IIPR were potential Bt strains isolated from Indo-Gangetic plains which is having both entomocidal and nematicidal properties. Further, these strains were deposited at national repository, ICAR-NBAIM with the following accession no.: NAIMCC-SB-065 and NAIMCC-SB-066, respectively. These strains can be formulated as microbial biopesticide.

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Characterization and mosquitocidal potency of a Bacillus thuringiensis strain of rice field soil of Burdwan, West Bengal, India

Cited by 5 publications

References 17 publications

Isolation of Mosquitocidal Bacteria from Soil Samples to Control Mosquito Vectors

Isolation of Mosquitocidal Bacteria from Soil Samples to Control Mosquito Vectors

Antifungal mechanisms of the antagonistic bacterium Bacillus mojavensis UTF-33 and its potential as a new biopesticide

Characterization of entomotoxic and nematotoxic genes from indigenous Bacillus thuringiensis strains and their biocontrol potential

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