Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.)

Fischer, Kristin; Dieterich, R.; Nelson, Matthew N.; Kamphuis, Lars G.; Singh, Karam B.; Rotter, Björn; Krezdorn, Nicolas; Winter, Peter; Wehling, Peter; Ruge-Wehling, Brigitte

doi:10.1007/s00122-015-2572-3

Cited by 26 publications

(36 citation statements)

References 28 publications

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“…The line is still used as a reference in phytopathological assays as its Phomopsis stem blight resistance has never been broken despite wide implementation of this genotype in Australian breeding programs (Shankar et al 1996(Shankar et al , 2002Stefanova and Buirchell 2010;Yang et al 2015Yang et al , 2013aYang et al , 2002. In Europe, D. toxica seems to be a dormant pathogen as reports of the disease date from as early as 1880 in Germany and in 1892 in Denmark (on L. angustifolius and L. luteus) but it has never caused serious problems (Fischer 1893;Lind 1913). Although it has appeared from time to time in different countries in Europe, including Poland and Russia (Lewartowska et al 1994;Marcinkowska 2007).…”

Section: Introductionmentioning

confidence: 99%

Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection

et al. 2019

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The fungus, Diaporthe toxica, anamorph Phomopsis sp., previously classified as P. leptostromiformis, is a plant endophyte and occasional pathogen, causing Phomopsis stem blight. This disease is damaging not only to lupins but also to the animals grazing on infected plants, due to the toxic secondary metabolites called phomopsins. The aim of this work was to validate markers for resistance to Phomopsis stem blight in narrow-leafed lupins and identify novel germplasm with increased levels of resistance to the disease. Plant inoculations were performed using ten isolates of D. toxica, originating from Australia and Poland. The European core collection of L. angustifolius was evaluated both in a controlled environment and with field experiments to classify the accessions based on their resistance to the disease. Simultaneously, the accessions were assayed with disease resistance markers to identify donors of hypothetical resistance alleles. We have found that the European lupin germplasm collection preserves wild and domesticated donors of at least two resistance genes to Phomopsis stem blight, including Phr1 and PhtjR. Molecular markers PhtjM7, InDel2, and InDel10, tagging PhtjR gene, were applicable for marker-assisted selection targeting the European gene pool with an expected accuracy of 95%. None of diagnostic markers for the Phr1 locus was found useful for European breeding programs; two existing markers Ph258M1 and Ph258M2 were unreliable, due to a high percentage of false-positive results (up to 58%) and a high recombination rate between markers (~30%).

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Section: Introductionmentioning

confidence: 99%

Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection

et al. 2019

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“…Further characterization of the in vitro anti-fungal properties of β6-conglutin was performed in a detailed time course experiment against two isolates (WAC8672 and WAC10444) of a major fungal pathogen of lupins, C. lupini which causes anthracnose disease (Fischer et al, 2015). Radial outgrowth of C. lupini mycelium on PDA plates toward Whatman filter disks containing control protein (BSA), β6-conglutin or no protein was recorded.…”

Section: Resultsmentioning

confidence: 99%

Narrow-Leafed Lupin (Lupinus angustifolius) β1- and β6-Conglutin Proteins Exhibit Antifungal Activity, Protecting Plants against Necrotrophic Pathogen Induced Damage from Sclerotinia sclerotiorum and Phytophthora nicotianae

et al. 2016

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Vicilins (7S globulins) are seed storage proteins and constitute the main protein family in legume seeds, particularly in narrow-leafed lupin (Lupinus angustifolius L.; NLL), where seven vicilin genes, called β1- to β7-conglutin have been identified. Vicilins are involved in germination processes supplying amino acids for seedling growth and plant development, as well as in some cases roles in plant defense and protection against pathogens. The roles of NLL β-conglutins in plant defense are unknown. Here the potential role of five NLL β-conglutin family members in protection against necrotrophic fungal pathogens was investigated and it was demonstrated that recombinant purified 6xHis-tagged β1- and β6-conglutin proteins exhibited the strongest in vitro growth inhibitory activity against a range of necrotrophic fungal pathogens compared to β2, β3, and β4 conglutins. To examine activity in vivo, two representative necrotrophic pathogens, the fungus Sclerotinia sclerotiorum and oomycete Phytophthora nicotianae were used. Transient expression of β1- and β6-conglutin proteins in Nicotiana benthamiana leaves demonstrated in vivo growth suppression of both of these pathogens, resulting in low percentages of hyphal growth and elongation in comparison to control treated leaves. Cellular studies using β1- and β6-GFP fusion proteins showed these conglutins localized to the cell surface including plasmodesmata. Analysis of cellular death following S. sclerotiorum or P. nicotianae revealed both β1- and β6-conglutins suppressed pathogen induced cell death in planta and prevented pathogen induced suppression of the plant oxidative burst as determined by protein oxidation in infected compared to mock-inoculated leaves.

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“…In prior research, selection markers for resistance to anthracnose have been developed including the loci LanrBo (Ruge‐Wehling, Dieterich, Thiele, Eickmeyer, & Wehling, ; Fischer et al., ) and Lanr1 (Yang, Boersma, You, Buirchell, & Sweetingham, ; Kamphuis et al., ). These molecular tools are now available to introduce known resistance genes into novel prebreeding lines like those identified in the present work.…”

Section: Discussionmentioning

confidence: 99%

“…In prior research, selection markers for resistance to anthracnose have been developed including the loci LanrBo (Ruge-Wehling, Dieterich, Thiele, Eickmeyer, & Wehling, 2009;Fischer et al, 2015) and…”

Section: Discussionmentioning

confidence: 99%

Generating genetic variation in narrow‐leafed lupin (Lupinus angustifolius L.) for plant architecture by ethyl methanesulfonate mutagenesis

et al. 2018

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Narrow‐leafed lupin (NLL) is a source of protein and fibre with exceptional functional properties useful for a variety of purposes. In adapted NLL germplasm selected for low alkaloid contents, the genetic base is narrow, thus limiting fast breeding progress. In an EMS‐based mutagenesis programme, we have treated 40,000 seeds of cv. ‘Boruta’ and obtained a variety of phenotypic mutant lines expressing novel growth types with altered plant height and/or branching. In 3‐year field trials at two locations, three of these lines exhibited significantly improved grain yield components compared to the cv. ‘Boruta’. Growth types of these promising lines were shown to be inherited in a monogenic recessive manner and should, thus, be easily fixable by breeding.

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Characterization and mapping of LanrBo: a locus conferring anthracnose resistance in narrow-leafed lupin (Lupinus angustifolius L.)

Cited by 26 publications

References 28 publications

Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection

Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection

Narrow-Leafed Lupin (Lupinus angustifolius) β1- and β6-Conglutin Proteins Exhibit Antifungal Activity, Protecting Plants against Necrotrophic Pathogen Induced Damage from Sclerotinia sclerotiorum and Phytophthora nicotianae

Generating genetic variation in narrow‐leafed lupin (Lupinus angustifolius L.) for plant architecture by ethyl methanesulfonate mutagenesis

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