Characterization and Mapping of Anthracnose Resistance Gene in Mesoamerican Common Bean Cultivar Crioulo 159

Abstract

The common bean cultivar Crioulo 159 provides a valuable source of resistance to Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara. The objective of this study was to characterize the genetic resistance of Crioulo 159 to C. lindemuthianum races 2, 64, 73, and 2047 through inheritance, allelism testing, and molecular analysis. Populations were obtained from crosses between Crioulo 159 and cultivars possessing anthracnose resistance genes. Inheritance tests performed in the F2 population and F2:3 families derived from Crioulo 159 (R, resistant) × Cornell 49–242 (S, susceptible) and inoculated with races 2047 and 73 showed segregation that fit ratios of 3R:1S and 1RR:2RS:1SS, respectively, indicating the action of a dominant resistance gene in Crioulo 159. The absence of recombination between two identical sets of F2:3 families inoculated with C. lindemuthianum race 2047 and 73, respectively, allowed the conclusion that a single dominant gene controls the resistance of Crioulo 159 to both races. Allelism tests revealed the independence of the gene in Crioulo 159 from those previously characterized for anthracnose resistance genes. Based on the results, we propose naming this newly discovered anthracnose resistance gene in Crioulo 159 as Co‐16. Molecular analyses revealed that sequence‐tagged site marker g2467900/800 is linked in coupling phase at 4.8 cM from the Co‐16 locus on chromosome Pv04. Additionally, molecular markers g2303, BARCPVSSR04561 and BARCPVSSR04570, which are linked to the Co‐34 allele in Ouro Negro, were not linked to the Co‐16 gene in Crioulo 159.

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

“…The common bean genome also contains NBS-LRR regions that are distributed across all chromosomes ( Ferrier-Cana et al, 2003 ; Richard et al, 2017 ; Wu et al, 2017 ) and a recent GWAS study showed associations between these NBS-LRR regions with anthracnose resistance ( Wu et al, 2017 ). Anthracnose resistance genes Co-3 and its alleles, Co-16 are mapped to common bean Pv04 and this study also found association of SNPs on Pv04 with anthracnose resistance to races 3, 87, and 503 ( Geffroy et al, 1999 ; David et al, 2008 ; Coimbra-Gonçalves et al, 2016 ; Murube et al, 2019 ). The Co-3 4 locus was previously mapped on the 3.36-Mb genomic position but it was recently mapped to the region between 0.49 to 0.58 Mb on Pv04 ( Valentini et al, 2017 ) which indicates shift in the physical positions with better mapping tools ( Kelly and Bornowski, 2018 ).…”

“…Anthracnose resistance genes Co-13 and Co-17 are mapped to Pv03 ( Goncalves-Vidigal et al, 2009 ; Lacanallo and Gonçalves-Vidigal, 2015 ; Trabanco et al, 2015 ). The anthracnose resistance gene mapped to Pv04 are Co-3 and its alleles, Co-10 , Co-y , Co-z Co-15 , Co-16 , and Co-RVI ( Geffroy et al, 2008 ; Coelho et al, 2013 ; Goncalves-Vidigal et al, 2013 ; Sousa et al, 2015 ; Coimbra-Gonçalves et al, 2016 ). Anthracnose resistance genes Co-5 , Co-6 , and Co-v map to Pv07 ( Campa et al, 2009 ).…”

Integration of anthracnose resistance loci and RLK and NBS‐LRR‐encoding genes in the Phaseolus vulgaris L. genome

“…In addition, alleles close to the Co‐3 locus were found in the Andean cultivars MDRK, Kaboon, Widusa, A 252, and Xana (Campa et al., 2014 ; Campa, Giraldez, & Ferreira, 2011 ; Campa, Trabanco, & Ferreira, 2017; Rodríguez‐Suárez, Ferreira, Campa, Pañeda, & Giraldez, 2008; Rodríguez‐Suárez, Méndez‐Vigo, Pañeda, Ferreira, & Giraldez, 2007 ). Interestingly, other genes such as Co‐y , Co‐z , Co‐15 , and Co‐16 (Coimbra‐Gonçalves et al., 2016; Geffroy et al., 1999; Sousa et al., 2015) are also present in Pv04, resulting in a cluster of anthracnose resistance genes in Cluster 4.1 (Figure 3). Genome‐wide association studies also identified an association of ANT resistance for Races 2, 4, 7, 9, 81 and 109 in Pv04 (Perseguini et al., 2016; Vaz Bisneta et al., 2019; Wu et al., 2017; Zuiderveen et al., 2016).…”

Alternative markers linked to the Phg-2 angular leaf spot resistance locus in common bean using the Phaseolus genes marker database

“…The PhaseolusGenes marker database has become an essential tool for cataloging bean marker diversity so that the breeding community can utilize marker information from multiple sources to facilitate MAS (Gonçalves-Vidigal et al, 2011Reinprecht et al, 2013;Oblessuc et al, 2013Oblessuc et al, , 2015Aranda et al, 2014;Keller et al, 2015;Sousa et al, 2015;Coimbra-Gonçalves et al, 2016;Perseguini et al, 2016). Because genomic resequencing of breeding parents is today a more routine practice with the lowered cost of sequencing, the PhaseolusGenes database should evolve from a marker database into a sequence database, which includes not only the current markers but also the increasingly large number (> 2,000) of genotypes that have been sequenced, including the 16 parental genotypes of the ABC projects (T. Miller and P. Gepts, unpubl.…”