Characterization and mapping of a putative laccase-like multicopper oxidase gene in the barley (Hordeum vulgare L.)

Tomková, Lenka; Kučera, L.; Vaculová, Kateřina; Milotová, J.

doi:10.1016/j.plantsci.2011.11.003

Cited by 3 publications

(7 citation statements)

References 57 publications

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“…We also observed high normalized levels of HvLac 1 transcript in the malt culms, corresponding to the finding that the HvLac1 gene is root specific . In malt culms, the detected level of HvLac 1 transcript was as high as the maximum values after 96 h of malting in the varieties Sebastian and Streif.…”

Section: Resultssupporting

confidence: 79%

“…The highest level of HvLac1 gene expression in all of the analysed barley varieties was observed at the end of steeping or on the first day of germination (96 h) (Figs 1 and 2). Although HvLac1 gene sequence variability was found between barley varieties, 28 any differences in the normalized transcript level during malting were not observed. During the steeping stage, water and air rest periods were used, which can simulate stress conditions.…”

Section: Rt-qpcrmentioning

confidence: 79%

“…We also observed high normalized levels of HvLac1 transcript in the malt culms, corresponding to the finding that the HvLac1 gene is root specific. 28 In malt culms, the detected level of HvLac1 transcript was as high as the maximum values after 96 h of malting in the varieties Sebastian and Streif. In the varieties recommended for the production of beer with the PGI 'Česke pivo' (Czech beer), the normalized transcript values were lower than were maximum transcript values after 72 h of malting (Figs 1 and 2).…”

Section: Rt-qpcrmentioning

confidence: 89%

“…A set of four barley varieties from the Research Institute of Brewing and Malting were selected: Bojos and Blaník with the ‘Haruna Nijo’ ( HN ) allele, and Sebastian and Streif with the ‘Morex’ (M) allele determining barley laccase . The barley varieties Bojos and Blaník are recommended for the production of beer with PGI ‘Česke pivo’ (Czech beer).…”

Section: Methodsmentioning

confidence: 99%

“…In brewing, laccase is used to prevent the formation of off‐flavour compounds, such as trans ‐2‐nonenal, and adding laccase may remove any excess oxygen, and at the same time the laccase will remove some of the polyphenols . Recently, new information about barley laccases has become accessible, but data about the activity of laccase genes in barley have not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

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Changes in polyphenol compounds and barley laccase expression during the malting process

et al. 2015

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Section: Resultssupporting

confidence: 79%

Section: Rt-qpcrmentioning

confidence: 79%

Section: Rt-qpcrmentioning

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Changes in polyphenol compounds and barley laccase expression during the malting process

et al. 2015

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Exploring the loblolly pine (Pinus taeda L.) genome by BAC sequencing and Cot analysis

Perera

Magbanua

Thummasuwan

et al. 2018

Gene

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Loblolly pine (LP; Pinus taeda L.) is an economically and ecologically important tree in the southeastern U.S. To advance understanding of the loblolly pine (LP; Pinus taeda L.) genome, we sequenced and analyzed 100 BAC clones and performed a Cot analysis. The Cot analysis indicates that the genome is composed of 57, 24, and 10% highly-repetitive, moderately-repetitive, and single/low-copy sequences, respectively (the remaining 9% of the genome is a combination of fold back and damaged DNA). Although single/low-copy DNA only accounts for 10% of the LP genome, the amount of single/low-copy DNA in LP is still 14 times the size of the Arabidopsis genome. Since gene numbers in LP are similar to those in Arabidopsis, much of the single/low-copy DNA of LP would appear to be composed of DNA that is both gene- and repeat-poor. Macroarrays prepared from a LP bacterial artificial chromosome (BAC) library were hybridized with probes designed from cell wall synthesis/wood development cDNAs, and 50 of the "targeted" clones were selected for further analysis. An additional 25 clones were selected because they contained few repeats, while 25 more clones were selected at random. The 100 BAC clones were Sanger sequenced and assembled. Of the targeted BACs, 80% contained all or part of the cDNA used to target them. One targeted BAC was found to contain fungal DNA and was eliminated from further analysis. Combinations of similarity-based and ab initio gene prediction approaches were utilized to identify and characterize potential coding regions in the 99 BACs containing LP DNA. From this analysis, we identified 154 gene models (GMs) representing both putative protein-coding genes and likely pseudogenes. Ten of the GMs (all of which were specifically targeted) had enough support to be classified as intact genes. Interestingly, the 154 GMs had statistically indistinguishable (α = 0.05) distributions in the targeted and random BAC clones (15.18 and 12.61 GM/Mb, respectively), whereas the low-repeat BACs contained significantly fewer GMs (7.08 GM/Mb). However, when GM length was considered, the targeted BACs had a significantly greater percentage of their length in GMs (3.26%) when compared to random (1.63%) and low-repeat (0.62%) BACs. The results of our study provide insight into LP evolution and inform ongoing efforts to produce a reference genome sequence for LP, while characterization of genes involved in cell wall production highlights carbon metabolism pathways that can be leveraged for increasing wood production.

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QTL for induced resistance against leaf rust in barley

Matros

Schikora²,

Ordon³

et al. 2023

Front. Plant Sci.

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Leaf rust caused by Puccinia hordei is one of the major diseases of barley (Hordeum vulgare L.) leading to yield losses up to 60%. Even though, resistance genes Rph1 to Rph28 are known, most of these are already overcome. In this context, priming may promote enhanced resistance to P. hordei. Several bacterial communities such as the soil bacterium Ensifer (syn. Sinorhizobium) meliloti are reported to induce resistance by priming. During quorum sensing in populations of gram negative bacteria, they produce N-acyl homoserine-lactones (AHL), which induce resistance in plants in a species- and genotype-specific manner. Therefore, the present study aims to detect genotypic differences in the response of barley to AHL, followed by the identification of genomic regions involved in priming efficiency of barley. A diverse set of 198 spring barley accessions was treated with a repaired E. meliloti natural mutant strain expR+ch producing a substantial amount of AHL and a transformed E. meliloti strain carrying the lactonase gene attM from Agrobacterium tumefaciens. For P. hordei resistance the diseased leaf area and the infection type were scored 12 dpi (days post-inoculation), and the corresponding relative infection and priming efficiency were calculated. Results revealed significant effects (p<0.001) of the bacterial treatment indicating a positive effect of priming on resistance to P. hordei. In a genome‐wide association study (GWAS), based on the observed phenotypic differences and 493,846 filtered SNPs derived from the Illumina 9k iSelect chip, genotyping by sequencing (GBS), and exome capture data, 11 quantitative trait loci (QTL) were identified with a hot spot on the short arm of the barley chromosome 6H, associated to improved resistance to P. hordei after priming with E. meliloti expR+ch. Genes in these QTL regions represent promising candidates for future research on the mechanisms of plant-microbe interactions.

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Characterization and mapping of a putative laccase-like multicopper oxidase gene in the barley (Hordeum vulgare L.)

Cited by 3 publications

References 57 publications

Changes in polyphenol compounds and barley laccase expression during the malting process

Changes in polyphenol compounds and barley laccase expression during the malting process

Exploring the loblolly pine (Pinus taeda L.) genome by BAC sequencing and Cot analysis

QTL for induced resistance against leaf rust in barley

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