Characterization and mapping of a spotted-leaf genotype, spl Y181 that confers blast susceptibility in rice

Sun, Huimin; Mao, Jiejing; Lan, Bo; Zhang, Chunjiao; Zhao, Chenchen; Pan, Gang; Pan, Xiaohua

doi:10.1007/s10658-014-0476-8

Cited by 6 publications

(2 citation statements)

References 37 publications

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“…It was discovered that there were great differences in the shape and color of diseaselike spots in different mutants. Spl36 [16] and spl(Y181) [17] showed irregular shapes, while spl24 [18,19] had regular shapes. The disease-like spots of SPL3 [20] and spl16 [21] were black, and those of spl18 [22] and lil1 [23] were reddish brown.…”

Section: Introductionmentioning

confidence: 96%

Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice

Li,

Xu,

Shui

et al. 2023

Agriculture

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This study was conducted on the lesion-mimic mutant lm5, which was produced by mutagenesis of WYJ21 (WT) using ethyl methane sulfonate (EMS). The mutant lm5 was short in the seedling stage and displayed yellowish-brown disease-like spots on leaves that were yellowish-brown when the plant was at the tillering stage. The disease-like spots gradually grew larger as the plant grew until it reached maturity. Compared to WT, lm5 had considerably reduced the plant height, ear panicle length, tiller number, and 1000-grain weight. A single recessive gene was found to be in control of lm5, according to a genetic study. It was physically located 245 kb apart between the RM21160 and RM180 markers on chromosome 7. Using RiceData and other websites, analyze and sequence potential gene candidates. Exon 7 of LOC_Os07g10390 (OsLM5) was identified to have a mutation that changed the 1560 base from G to A, changing the 788 amino acids from Arg to Lys. The OsLM5 gene was found to be a new allele of the SPL5 gene, encoding the protein shear factor SF3b3. Studies showed that OsLM5 was localized in the nucleus, and OsLM5 was significantly expressed in leaves. Reactive oxygen species (ROS) accumulation occurred in the leaves and roots of mutant lm5, and qPCR results showed abnormal expression of genes related to chloroplast development as well as significantly increased expression of genes related to aging and disease course. The OsLM5 gene may have a significant impact on the regulation of apoptosis in rice cells.

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Section: Introductionmentioning

confidence: 96%

Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice

Li,

Xu,

Shui

et al. 2023

Agriculture

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“…The hypersensitive response (HR) is one of the most common and effective defense responses to pathogen attack (Chen et al 2013). HR is regarded as the most efficient defense mechanism because of its rapid physiological changes that arrest the multiplication or expansion of the pathogens in the host cell to prevent their further spread to adjacent cells (Sun et al 2014). Research has found that HR-like symptoms can also be activated in the absence of a pathogen attack, resulting in lesion-like appearances (Huang et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Research Article Genome-Wide Analysis of a Radiation-Induced Lesion Mimic Mutant in Rice

Zhenga¹,

Guoa²,

Wua³

et al. 2017

Genet. Mol. Res.

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Lesion mimic mutations (LMMs) are very useful genetic tools in plants, however, it is difficult to uncover genome-wide mutations using traditional technology, the development of whole genome sequencing (WGS) provides a means by which to identify genome-wide LMMs. In this study, WGS was performed to reveal the molecular mechanisms behind LMMs in rice. An LMM JA001 was derived from indica rice IR64 by treating with 60Coγ-radiation. Solexa sequencing technology was applied to uncover the mutations. In the JA001 genome, 7.93% of the genome sequences were altered in the JA001 genome, among which were 351323 single nucleotide polymorphisms and 32,648 bp indel variations in 19,937 genes. Gene ontology clustering revealed that genes that are associated with binding, kinase activity, the oxoglutarate dehydrogenase complex, the beta-galactosidase complex, protein phosphorylation, and cell-pollen recognition had a high mutation rate. It was also predicted that five mutated genes were involved in the porphyrin and chlorophyll metabolism, Huimin Sun, et al. 2 Genetics and Molecular Research 16 (4): gmr16039812 carotenoid biosynthesis, and plant-pathogen interaction pathways. Genome-wide analysis of mutations provides new insights into the molecular mechanisms of LMMs mutagenesis

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Transcriptome analysis of Dlm mutants reveals the potential formation mechanism of lesion mimic in wheat

Shi

Zheng

et al. 2016

Eur J Plant Pathol

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Characterization and mapping of a spotted-leaf genotype, spl Y181 that confers blast susceptibility in rice

Cited by 6 publications

References 37 publications

Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice

Phenotypic Analysis and Gene Cloning of a New Allelic Mutant of SPL5 in Rice

Research Article Genome-Wide Analysis of a Radiation-Induced Lesion Mimic Mutant in Rice

Transcriptome analysis of Dlm mutants reveals the potential formation mechanism of lesion mimic in wheat

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