Characterization and localization of cocaine- and amphetamine-regulated transcript (CART) binding sites

Keller, Patrick; Compan, Valérie; Bockaert, Joël; Giacobino, Jean-Paul; Charnay, Yves; Bouras, Constantin; Assimacopoulos-Jeannet, F.

doi:10.1016/j.peptides.2005.10.016

Cited by 31 publications

(18 citation statements)

References 33 publications

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“…An inhibition of L-type voltage-gated calcium channels by CART peptide has been reported in cultured rat hippocampal neurons [ 40 ]. Specific CART peptide receptor binding has been identified in AtT20 cells [ 36 ], PC12 cells [ 27 ], HepG2 cells, hypothalamic dissociated cells [ 20 ], and in primary cultures of NAcc neurons [ 19 ]. Binding studies indicate that the CART receptor is a GPCR, which utilizes a pertussus toxin sensitive Gi/o coupling.…”

Section: Discussionmentioning

confidence: 99%

“…Rats were then switched to a progressive ratio (PR) schedule of reinforcement. Under this schedule, response requirements for each successive presentation of a reinforcer increased progressively in the following series: 1, 2, 4,6,9,12,15,20,25,32,40,50,62,77,95,118,145,178,219,268,328,402,492,603,737,901. Break point (BP) was determined in the cocaine experiment as the last ration achieved before 1 hr had lapsed without reinforcement with a maximum session length of 5 hr.…”

Section: Cocaine and Food Self-administration Training And Testingmentioning

confidence: 99%

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Injection of CART (cocaine- and amphetamine-regulated transcript) peptide into the nucleus accumbens reduces cocaine self-administration in rats

Jaworski

Hansen

Kuhar

et al. 2008

Behavioural Brain Research

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Cocaine-and amphetamine-regulated transcript (CART) peptides appear to modulate various effects of psychostimulant drugs. Injections of CART peptide into the nucleus accumbens (NAcc) inhibit locomotion produced by systemic injections of the psychostimulants cocaine and amphetamine. Intra-NAcc injections of CART peptide also inhibit locomotion produced by microinfusions of dopamine into the NAcc, suggesting that the effects of CART peptides may be due to an interaction with the dopaminergic system in the NAcc. We sought to determine if this inhibitory effect of CART peptide generalizes to other measures of dopaminergic function such as reward/reinforcement by testing the effect of bilateral intra-NAcc CART infusions (0, 0.25, 1.0 and 2.5 µg per side) on cocaine and food self-administration. One group of rats self-administered cocaine (0.75 mg/kg per 140 µl IV infusion) on a progressive ratio schedule. A separate group received 45 mg food pellets on the same progressive ratio schedule. Bilateral intra-NAcc injections of CART peptide dose-dependently decreased the number of cocaine infusions, the breakpoint of cocaine self-administration, and the total number of bar presses on the cocaine-associated lever. There were no effects of CART injections on the break point for food reward. Thus, we conclude that injections of CART into the NAcc appear to functionally antagonize a major site of action for cocaine self-administration in rats.

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Section: Discussionmentioning

confidence: 99%

Section: Cocaine and Food Self-administration Training And Testingmentioning

confidence: 99%

Injection of CART (cocaine- and amphetamine-regulated transcript) peptide into the nucleus accumbens reduces cocaine self-administration in rats

Jaworski

Hansen

Kuhar

et al. 2008

Behavioural Brain Research

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show abstract

“…Specific binding sites have recently been reported for CART in brain and cell lines (34,35), although no receptor or binding partner has yet been identified. There are multiple actions of CART that can potentially explain its neuroprotective effect, including inhibition of calcium signaling (36), CREB phosphorylation (37), and activation of the ERK͞MAPK pathway (38).…”

Section: Discussionmentioning

confidence: 99%

Role of cocaine- and amphetamine-regulated transcript in estradiol-mediated neuroprotection

Zhang²,

Klaus³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

107

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Estrogen reduces brain injury after experimental cerebral ischemia in part through a genomic mechanism of action. Using DNA microarrays, we analyzed the genomic response of the brain to estradiol, and we identified a transcript, cocaine-and amphetamine-regulated transcript (CART), that is highly induced in the cerebral cortex by estradiol under ischemic conditions. Using in vitro and in vivo models of neural injury, we confirmed and characterized CART mRNA and protein up-regulation by estradiol in surviving neurons, and we demonstrated that i.v. administration of a rat CART peptide is protective against ischemic brain injury in vivo. We further demonstrated binding of cAMP response element (CRE)-binding protein to a CART promoter CRE site in ischemic brain and rapid activation by CART of ERK in primary cultured cortical neurons. The findings suggest that CART is an important player in estrogen-mediated neuroprotection and a potential therapeutic agent for stroke and other neurodegenerative diseases.ischemia ͉ stroke ͉ estrogen

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“…Binding of a CART-GFP fusion protein to HepG2 cells and dissociated hypothalamic cells has been reported previously [20]. Specific saturable binding of CART peptides to AtT20 cells [21], PC12 cells [22], and primary cultures of cells from rat nucleus accumbens [23] have been demonstrated, and CART binding is reduced in the presence of GTP, but not ATP, analogues [23].…”

Section: Introductionmentioning

confidence: 68%

Regulation of Granulosa Cell Cocaine and Amphetamine Regulated Transcript (CART) Binding and Effect of CART Signaling Inhibitor on Granulosa Cell Estradiol Production During Dominant Follicle Selection in Cattle1

Folger

Jimenez-Krassel

Ireland

et al. 2013

Biology of Reproduction

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We previously established a potential role for cocaine and amphetamine regulated transcript (CARTPT) in dominant follicle selection in cattle. CARTPT expression is elevated in subordinate versus dominant follicles, and treatment with the mature form of the CARTPT peptide (CART) decreases follicle-stimulating hormone (FSH)-stimulated granulosa cell estradiol production in vitro and follicular fluid estradiol and granulosa cell CYP19A1 mRNA in vivo. However, mechanisms that regulate granulosa cell CART responsiveness are not understood. In this study, we investigated hormonal regulation of granulosa cell CART-binding sites in vitro and temporal regulation of granulosa cell CART-binding sites in bovine follicles collected at specific stages of a follicular wave. We also determined the effect of inhibition of CART receptor signaling in vivo on estradiol production in future subordinate follicles. Granulosa cell CART binding in vitro was increased by FSH, and this induction was blocked by estrogen receptor antagonist treatment. In follicles collected in vivo at specific stages of a follicular wave, granulosa cell CART binding in the F2 (second largest), future subordinate follicle increased during dominant follicle selection. Injection into the F2 follicle (at onset of diameter deviation) of an inhibitor of the o/i subclass of G proteins (previously shown to block CART actions in vitro) resulted in increased follicular fluid estradiol concentrations in vivo. Collectively, results demonstrate hormonal regulation of granulosa cell CART binding in vitro and temporal regulation of CART binding in subordinate follicles during dominant follicle selection. Results also suggest that CART signaling may help suppress estradiol-producing capacity of the F2 (subordinate) follicle during this time period.

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Characterization and localization of cocaine- and amphetamine-regulated transcript (CART) binding sites

Cited by 31 publications

References 33 publications

Injection of CART (cocaine- and amphetamine-regulated transcript) peptide into the nucleus accumbens reduces cocaine self-administration in rats

Injection of CART (cocaine- and amphetamine-regulated transcript) peptide into the nucleus accumbens reduces cocaine self-administration in rats

Role of cocaine- and amphetamine-regulated transcript in estradiol-mediated neuroprotection

Regulation of Granulosa Cell Cocaine and Amphetamine Regulated Transcript (CART) Binding and Effect of CART Signaling Inhibitor on Granulosa Cell Estradiol Production During Dominant Follicle Selection in Cattle1

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