Characterization and localization of CD38 in the vertebrate eye

Khoo, Keng Meng; Chang, Chan Fong

doi:10.1016/s0006-8993(98)01347-x

Cited by 43 publications

(19 citation statements)

References 45 publications

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“…Our results indicate that CD38 expression in the tiger salamander retina is restricted to Mü ller cells; the labeling pattern we observed confirms and extends results showing CD38 expression in unidentified cells of the rat retina (Khoo and Chang, 1999). As in other cell types expressing this protein, CD38 was found only on the plasma membrane.…”

Section: Potential Ca 2؉ Release Mechanismssupporting

confidence: 89%

CD38 expression and NAD⁺‐induced intracellular Ca²⁺ mobilization in isolated retinal Müller cells

Esguerra

Miller²

2002

Glia

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Müller cells of the vertebrate retina are prominent radial glia that provide essential support to sustain homeostasis of the tissue, including redistribution of external potassium, uptake and metabolism of neurotransmitters, and secretion of factors that stabilize the retina. Meeting this diversity of functional supports requires that Müller cells express numerous receptors, transporters, enzymes, and tissue factors. In this study, we provide evidence that adds to the dimensions of Müller cell function by demonstrating a unique relationship between external NAD(+) and the mobilization of internal calcium, expressed in the form of calcium waves. The cellular mechanism that supports internal mobilization of calcium appears to depend on a complex multifunctional ectoenzyme, CD38, which converts NAD(+) into the intracellular Ca(2+)-mobilizing second-messenger cyclic ADP-ribose (cADPR) and could function as a detector for extracellular NAD(+), thus providing a novel signal detection system for evaluating the extracellular environment. Our results are consistent with a model of intracellular Ca(2+) mobilization in which membrane-bound CD38 binds extracellular NAD(+) and triggers intracellular Ca(2+) waves either by direct conversion of NAD(+) to cADPR or by activating intracellular cADPR synthesis. Our preliminary results indicate that the Ca(2+) waves induced by external NAD(+) propagate through an internal pathway that depends on the activation of ryanodine receptors, which appear to be distributed throughout the Müller cell cytosol. Because NAD(+) is likely to be enhanced when cells are stress or damaged, CD38 could enable Müller cells to detect NAD(+) under these circumstances and respond appropriately. Alternatively, NAD(+) could also represent a novel extracellular, paracrine function that mediates signaling between glial cells and/or other cellular elements of the retina.

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Section: Potential Ca 2؉ Release Mechanismssupporting

confidence: 89%

CD38 expression and NAD⁺‐induced intracellular Ca²⁺ mobilization in isolated retinal Müller cells

Esguerra

Miller²

2002

Glia

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“…Many genes that were not readily apparent by visual inspection were also identified through clustering. These genes included, for example, a cell surface molecule (CD38 antigen) that has been shown to be present in both the pigmented and non-pigmented ciliary body (Khoo and Chang, 1999) and many uncharacterized RIKEN cDNAs (Supp. Fig.…”

Section: Analysis Of Transcripts Expressed In the Mouse Peripheral Mamentioning

confidence: 99%

Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Trimarchi

Cho

Cepko

2009

Developmental Dynamics

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Specification of the peripheral optic cup by Wnt signaling is critical for formation of the ciliary body/iris. Identification of marker genes for this region during development provides a starting point for functional analyses. During transcriptional profiling of single cells from the developing eye, two cells were identified that expressed genes not found in most other single cell profiles. In situ hybridizations demonstrated that many of these genes were expressed in the peripheral optic cup in both early mouse and chicken development, and in the ciliary body/iris at subsequent developmental stages. These analyses indicate that the two cells probably originated from the developing ciliary body/iris. Changes in expression of these genes were assayed in embryonic chicken retinas when canonical Wnt signaling was ectopically activated by CA-␤-catenin. Twelve ciliary body/iris genes were identified as upregulated following induction, suggesting they are excellent candidates for downstream effectors of Wnt signaling in the optic cup. Developmental

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“…Both phospholipase C (Vivekanandan and Lou, 1989) and ADP-ribosyl cyclase (Khoo and Chang, 1999) are expressed in lens. Moreover, lens phospholipase C is activated by growth factors that initiate calcium waves, such as EGF and PDGF (Vivekanandan and Lou, 1989).…”

Section: Calcium In Lens Epithelial Cell Migrationmentioning

confidence: 99%

Regulation of cell adhesion and migration in lens development

Zelenka¹

2004

Int. J. Dev. Biol.

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Cell movements during lens development and differentiation involve dynamic regulation of cell-matrix and cell-cell adhesion. How these processes are regulated depends on the particular array of matrix components and adhesion proteins that are expressed, as well as the signaling pathways that affect them. This review examines what is known about adhesion proteins and their regulation in the lens in light of recent findings about the mechanism of cell migration. The characteristic shape and organization of the lens depends on highly regulated cell movements during development and differentiation. Epithelial cells at the equator migrate posteriorly, bringing them into contact with factors in the vitreous humor and initiating differentiation. Elongation of the differentiating fiber cells is coupled with directed migration, posteriorly along the capsule and anteriorly along the fiber cell-epithelial interface, to generate a symmetrically organized fiber cell mass with aligned suture planes. To make these movements, cells systematically create and dissolve cell-cell and cell-matrix adhesions, form connections between these adhesions and the cytoskeleton, and generate contractile force. Since errors in cell migration may lead to aberrant lens shape or misplacement of the lens sutures, precise regulation of each step is essential for the optical quality of the lens. Recent advances in cellular developmental biology have begun to shed light on the molecular mechanisms underlying cell movements and the changes in adhesion that make them possible. This review will summarize those findings and relate them to relevant studies of the lens to provide an outline of the cellular events that lead to lens morphogenesis.

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Characterization and localization of CD38 in the vertebrate eye

Cited by 43 publications

References 45 publications

CD38 expression and NAD⁺‐induced intracellular Ca²⁺ mobilization in isolated retinal Müller cells

CD38 expression and NAD⁺‐induced intracellular Ca²⁺ mobilization in isolated retinal Müller cells

Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Regulation of cell adhesion and migration in lens development

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Characterization and localization of CD38 in the vertebrate eye

Cited by 43 publications

References 45 publications

CD38 expression and NAD+‐induced intracellular Ca2+ mobilization in isolated retinal Müller cells

CD38 expression and NAD+‐induced intracellular Ca2+ mobilization in isolated retinal Müller cells

Identification of genes expressed preferentially in the developing peripheral margin of the optic cup

Regulation of cell adhesion and migration in lens development

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CD38 expression and NAD⁺‐induced intracellular Ca²⁺ mobilization in isolated retinal Müller cells

CD38 expression and NAD⁺‐induced intracellular Ca²⁺ mobilization in isolated retinal Müller cells