Characterization and in vitro activities of cell-free antigens from Histoplasma capsulatum-loaded biodegradable microspheres

Santos, Daniela Soares; Nicolete, Roberto; Souza, Patrícia Jorge Soalheiro de; Bitencourt, Cláudia da Silva; Santos, Ramon Rodrigues dos; Bonato, Vânia Luiza Deperon; Silva, Célio Lopes; Faccioli, Lúcia Helena

doi:10.1016/j.ejps.2009.10.003

Cited by 13 publications

(6 citation statements)

References 32 publications

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“…Maximal aspirator flow was used to compensate the low yield caused by high spray flow. PLGA particles were frequently spheres prepared by the double emulsion-solvent evaporation method (21,37). In contrast, particles prepared by primary emulsion/spray-drying method showed irregular surface morphology, in agreement with previous report (19,38).…”

Section: Discussionsupporting

confidence: 75%

PLGA Microparticles in Respirable Sizes Enhance an In Vitro T Cell Response to Recombinant Mycobacterium Tuberculosis Antigen TB10.4-Ag85B

Shi

Hickey

2009

Pharm Res

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Section: Discussionsupporting

confidence: 75%

PLGA Microparticles in Respirable Sizes Enhance an In Vitro T Cell Response to Recombinant Mycobacterium Tuberculosis Antigen TB10.4-Ag85B

Shi

Hickey

2009

Pharm Res

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“…Other proteins, such as fibroblast growth factor 9 (FGF9) and vaccination antigens, have also been proven to retain their biological activity in PEA-based carriers (dos Santos et al, 2009;Said et al, 2014). As further proof of CXCL1 activity after release, we observed significant in vivo evidence of microsphere released CXCL1.…”

Section: Discussionsupporting

confidence: 53%

CXCL1 microspheres: a novel tool to stimulate arteriogenesis

Caolo

Vries

Zupancich³

et al. 2015

Drug Delivery

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Context: After arterial occlusion, diametrical growth of pre-existing natural bypasses around the obstruction, i.e. arteriogenesis, is the body's main coping mechanism. We have shown before that continuous infusion of chemokine (C-X-C motif) ligand 1 (CXCL1) promotes arteriogenesis in a rodent hind limb ischemia model. Objective: For clinical translation of these positive results, we developed a new administration strategy of local and sustained delivery. Here, we investigate the therapeutic potential of CXCL1 in a drug delivery system based on microspheres. Materials and methods: We generated poly(ester amide) (PEA) microspheres loaded with CXCL1 and evaluated them in vitro for cellular toxicity and chemokine release characteristics. In vivo, murine femoral arteries were ligated and CXCL1 was administered either intra-arterially via osmopump or intramuscularly encapsulated in biodegradable microspheres. Perfusion recovery was measured with Laser-Doppler. Results: The developed microspheres were not cytotoxic and displayed a sustained chemokine release up to 28 d in vitro. The amount of released CXCL1 was 100-fold higher than levels in native ligated hind limb. Also, the CXCL1-loaded microspheres significantly enhanced perfusion recovery at day 7 after ligation compared with both saline and non-loaded conditions (55.4 ± 5.0% CXCL1-loaded microspheres versus 43.1 ± 4.5% non-loaded microspheres; n ¼ 8-9; p50.05). On day 21 after ligation, the CXCL1-loaded microspheres performed even better than continuous CXCL1 administration (102.1 ± 4.4% CXCL1-loaded microspheres versus 85.7 ± 4.8% CXCL1 osmopump; n ¼ 9; p50.05). Conclusion: Our results demonstrate a proof of concept that sustained, local delivery of CXCL1 encapsulated in PEA microspheres provides a new tool to stimulate arteriogenesis in vivo.

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“…Microparticles were prepared using an oil-in-water (O/W) emulsion solvent extraction-evaporation process as previously described [15,16]. In brief, 10 ml of methylene dichloride containing 30 mg of PLGA were homogenized.…”

Section: Micro and Nanoparticles Preparationmentioning

confidence: 99%

The uptake of PLGA micro or nanoparticles by macrophages provokes distinct in vitro inflammatory response

Nicolete

Santos

Faccioli

2011

International Immunopharmacology

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125

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Biodegradable micro/nanoparticles generated from PLGA have recently attracted attention due to their clinically proven biocompatibility, especially for immunization purposes. These polymeric particulate delivery systems are able to present antigens and activate both humoral and cellular responses. Many studies have discussed the ideal size of these particles in contributing to the generation of the different types of immune response. However, these studies do not demonstrate the effect of micro or nanoparticles, without any encapsulated bioactive, on phagocytic cells after the uptake process. In this context, the aim of this study was to analyze the in vitro inflammatory behavior of J774 murine macrophages after particles' uptake, since nano/microparticles per se can differently activate phagocytic cells, using or not appropriate receptors, inducing distinct inflammatory responses. An o/w emulsion solvent extraction-evaporation method was chosen to prepare the particles. We determined their diameters, zeta potential and morphology. Fluorescent particles' uptake by J774 murine "macrophage-like" cells was also analyzed. To evaluate the in vitro inflammatory profile of these cells after micro or nanoparticles' uptake, we conducted NF-κB translocation assay by confocal microscopy and also determined the pro-inflammatory cytokines production provoked by the particles.

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Characterization and in vitro activities of cell-free antigens from Histoplasma capsulatum-loaded biodegradable microspheres

Cited by 13 publications

References 32 publications

PLGA Microparticles in Respirable Sizes Enhance an In Vitro T Cell Response to Recombinant Mycobacterium Tuberculosis Antigen TB10.4-Ag85B

PLGA Microparticles in Respirable Sizes Enhance an In Vitro T Cell Response to Recombinant Mycobacterium Tuberculosis Antigen TB10.4-Ag85B

CXCL1 microspheres: a novel tool to stimulate arteriogenesis

The uptake of PLGA micro or nanoparticles by macrophages provokes distinct in vitro inflammatory response

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