Characterization and immunogenicity of the heat shock protein, hsp60 of Brucella abortus

Gor, Dennis O.

doi:10.31274/rtd-180813-10325

Cited by 1 publication

(1 citation statement)

References 89 publications

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“…Antibodies used for the Western blots were polyclonal rabbit antisera. Antisera for this study were developed in our laboratory and described in detail previously (10,16,27,29,62). The Western blot procedure used was based on that of Towbin et al (72).…”

Section: Methodsmentioning

confidence: 99%

Regulation of Brucella abortus Catalase

2000

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All aerobic organisms have mechanisms that protect against oxidative compounds. Catalase, peroxidase, superoxide dismutase, glutathione, and thioredoxin are widely distributed in many taxa and constitute elements of a nearly ubiquitous antioxidant metabolic strategy. Interestingly, the regulatory mechanisms that control these elements are rather different depending on the nature of the oxidative stress and the organism. Catalase is well documented to play an important role in protecting cells from oxidative stress. In particular, pathogenic bacteria seem to use this enzyme as a defensive tool against attack by the host. To investigate the significance of catalase in hostile environments, we made catalase deletion mutations in two different B. abortus strains and used two-dimensional gel analysis, survival tests, and adaptation experiments to explore the behavior and role of catalase under several oxidative stress conditions. These studies show that B. abortus strains that do not express catalase activity exhibit increased sensitivity to hydrogen peroxide. We also demonstrate that catalase expression is regulated in this species, and that preexposure to a sublethal concentration of hydrogen peroxide allows B. abortus to adapt so as to survive subsequent exposure to higher concentrations of hydrogen peroxide.

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