Characterization and Immunochemical Quantitation of Human Uromucoid

Quantitative immunoelectrophoretic assay for murine oncornavirus p30: noncovalent facilitation by sodium dodecyl sulfate

“…Several reports have been made of SDS causing nonspecific precipitation of normal serum proteins (especially 7S immunoglobulin) during agar gel diffusion and IEP of SDS solutions (5,11,19). In contrast, Crumpton and Parkhouse (8) reported that precipitation of antigen-antibody complexes was markedly inhibited by 0.5% SDS.…”

“…Investigators studying uromucoid have used the dissociating properties of SDS to facilitate a variety of immunological techniques (11,15). Because native uromucoid possesses a molecular weight of 7 x 106, the large glycoprotein molecule must first be dissociated into its 80,000-dalton subunits before analysis by agar gel immunodiffusion and IEP is possible (11). The investigators titrated the SDS and determined that 0.3% SDS was sufficient to maintain the uromucoid in its subunit state, thereby allowing RID and RIEP analyses to be performed.…”