Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome

Rodríguez-Ortega, Manuel J.; Norais, Nathalie; Bensi, Giuliano; Liberatori, Sabrina; Capo, Sabrina; Mora, Marirosa; Scarselli, María; Doro, Francesco; Ferrari, Germano; Garaguso, Ignazio; Maggi, Tiziana; Neumann, Anita; Covre, Alessia; Telford, John L.; Grandi, Guido

doi:10.1038/nbt1179

Cited by 389 publications

(445 citation statements)

References 48 publications

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“…Conjecture exists in the scientific literature regarding the biological relevance and certitude of surface expression of anchorless proteins in GAS [5,30]. To validate our findings and to test protective vaccine efficacy of the selected anchorless surface proteins, full-length M1 protein, ADI, TF and FBA were adjuvanted with Complete Freund's Adjuvant (CFA) and used to subcutaneously immunize BALB/c mice and the survival recorded following lethal intraperitoneal M1 GAS challenge.…”

Section: Anchorless Proteins Elicit Protective Immunitymentioning

confidence: 82%

Conserved anchorless surface proteins as group A streptococcal vaccine candidates

et al. 2012

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Section: Anchorless Proteins Elicit Protective Immunitymentioning

confidence: 82%

Conserved anchorless surface proteins as group A streptococcal vaccine candidates

et al. 2012

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“…In relation to this, with the aim of determining the protein profile of the B. longum envelope, and as a first approach to undertaking deeper functional studies, we analysed different subcellular fractions. The application of gel-based and gel-free technologies, combined with high-throughput techniques, allowed us to identify 218 proteins; about 70 % of them were predicted to be, or were previously described as being, in the cell envelope of Gram-positive bacteria (Antikainen et al, 2007;Candela et al, 2007;Eymann et al, 2004;Granato et al, 2004;Jang & Hanash 2003;Kelly et al, 2005b;Nandakumar et al, 2005;Rivera-Amill et al, 2001;Rodríguez-Ortega et al, 2006;Schaumburg et al, 2004;Severin et al, 2007;Silveira et al, 2004;Tjalsma et al, 2008;Wolff et al, 2007). Furthermore, 48 of them are predicted to be integral membrane proteins (contain hypothetical transmembrane segments) and 30 of them have different extracytoplasmic sorting signals.…”

Section: Discussionmentioning

confidence: 99%

The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment

et al. 2009

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Host-bacteria interactions are often mediated via surface-associated proteins. The identification of these proteins is an important goal of bacterial proteomics. To address how bile can influence the cell-envelope proteome of Bifidobacterium longum biotype longum NCIMB 8809, we analysed its membrane protein fraction using stable isotope labelling of amino acids in cell culture (SILAC). We were able to identify 141 proteins in the membrane fraction, including a large percentage of the theoretical transporters of this species. Moreover, the envelope-associated soluble fraction was analysed using different subfractionation techniques and differential in-gel fluorescence electrophoresis (DIGE). This approach identified 128 different proteins. Some of them were well-known cell wall proteins, but others were highly conserved cytoplasmic proteins probably displaying a 'moonlighting' function. We were able to identify 11 proteins in the membrane fraction and 6 proteins in the envelope-associated soluble fraction whose concentration varied in the presence of bile. Bile promoted changes in the levels of proteins with important biological functions, such as some ribosomal proteins and enolase. Also, oligopeptide-binding proteins were accumulated on the cell surface, which was reflected in a different tripeptide transport rate in the cells grown with bile. The data reported here will provide the first cell-envelope proteome map for B. longum, and may contribute to understanding the bile tolerance of these bacteria

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“…Therefore, the development of efficacious vaccines against obligate and facultative intracellular pathogens depends largely upon the ability to identify antigen formulations that induce effective B-and T-cell responses (1)(2)(3). Although both genomic and proteomic strategies have been applied successfully to discover B-cell-stimulating vaccines (4)(5)(6)(7), the identification of antigens eliciting effector T cells generally is considered less amenable to high-throughput approaches.…”

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confidence: 99%

Approach to discover T- and B-cell antigens of intracellular pathogens applied to the design of Chlamydia trachomatis vaccines

Finco

Frigimelica

Buricchi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Natural immunity against obligate and/or facultative intracellular pathogens is usually mediated by both humoral and cellular immunity. The identification of those antigens stimulating both arms of the immune system is instrumental for vaccine discovery. Although high-throughput technologies have been applied for the discovery of antibody-inducing antigens, few examples of their application for T-cell antigens have been reported. We describe how the compilation of the immunome, here defined as the pool of immunogenic antigens inducing T- and B-cell responses in vivo, can lead to vaccine candidates against Chlamydia trachomatis . We selected 120 C. trachomatis proteins and assessed their immunogenicity using two parallel high-throughput approaches. Protein arrays were generated and screened with sera from C. trachomatis -infected patients to identify antibody-inducing antigens. Splenocytes from C. trachomatis -infected mice were stimulated with 79 proteins, and the frequency of antigen-specific CD4 + /IFN-γ + T cells was analyzed by flow cytometry. We identified 21 antibody-inducing antigens, 16 CD4 + /IFN-γ + –inducing antigens, and five antigens eliciting both types of responses. Assessment of their protective activity in a mouse model of Chlamydia muridarum lung infection led to the identification of seven antigens conferring partial protection when administered with LTK63/CpG adjuvant. Protection was largely the result of cellular immunity as assessed by CD4 + T-cell depletion. The seven antigens provided robust additive protection when combined in four-antigen combinations. This study paves the way for the development of an effective anti- Chlamydia vaccine and provides a general approach for the discovery of vaccines against other intracellular pathogens.

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Characterization and identification of vaccine candidate proteins through analysis of the group A Streptococcus surface proteome

Cited by 389 publications

References 48 publications

Conserved anchorless surface proteins as group A streptococcal vaccine candidates

Conserved anchorless surface proteins as group A streptococcal vaccine candidates

The cell-envelope proteome of Bifidobacterium longum in an in vitro bile environment

Approach to discover T- and B-cell antigens of intracellular pathogens applied to the design of Chlamydia trachomatis vaccines

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