Characterization and Identification of Single Nucleotide Polymorphism Within the IGF‐1R Gene Associated with Growth Traits of Odontobutis potamophila

Abstract

IGF-1R plays a critical role in many physiological activities, such as development, growth, metabolism, and reproduction. Identification of genetic markers in the IGF-1R gene significantly associated with important growth traits of Odontobutis potamophila may accelerate genetic improvement. In this study, we characterized the cDNA sequence of the IGF-1R gene of O. potamophila, and our data revealed that IGF-1R contained an open reading frame of 1650 bp, encoding a polypeptide of 549 amino acids (aa). Quantitative real-time polymerase chain reaction demonstrated that IGF-1R was ubiquitously expressed in all tested tissues, and the most predominant expression of IGF-1R was detected in gonad. Moreover, IGF-1R was expressed at different developmental stages from embryo to larva, and its highest expression level was found at the morula stage. In addition, we identified three single nucleotide polymorphisms (SNPs; 1777T>C, 1704G>A, and 1208G>A) within IGF-1R locus and found that one SNP (1208G>A) was significantly associated with important growth traits of O. potamophila. Results showed that the overall length, body length, trunk width, caudal peduncle length, body weight, and body width in fish with AG genotype were significantly greater compared with those with GG genotype (P < 0.05). However, the other two SNPs (1777T>C and 1704G>A) did not show significant associations with any growth traits. Therefore, SNPs within the IGF-1R locus could be used as a selection criterion to improve growth traits of O. potamophila.

Progress in Aquaculture Genetics and Breeding in China

“…Another endemic Chinese species that has attracted more recent attention is the freshwater sleeper, Odontobutis potamophila . H. Zhang, Zhang et al (), H. Zhang, Zhao et al (), and X. Zhang et al () identified and characterized single nucleotide polymorphisms (SNPs) of insulin‐like growth factor 1 receptor (IGF‐1R) genes in O. potamophila . They found that a SNP site in the intron of IGF‐1R was associated with growth performance and suggested that this SNP site could be used in marker‐assisted selection (MAS) (H. Zhang, Zhang et al ; H. Zhang, Zhao et al ; X. Zhang et al ).…”

“…H. Zhang, Zhang et al (), H. Zhang, Zhao et al (), and X. Zhang et al () identified and characterized single nucleotide polymorphisms (SNPs) of insulin‐like growth factor 1 receptor (IGF‐1R) genes in O. potamophila . They found that a SNP site in the intron of IGF‐1R was associated with growth performance and suggested that this SNP site could be used in marker‐assisted selection (MAS) (H. Zhang, Zhang et al ; H. Zhang, Zhao et al ; X. Zhang et al ). Likewise, microsatellite loci were examined for potential use in MAS in pearl oyster, Pinctada fucata martensii , which is an important commercial bivalve used for the production of marine pearls in China and Japan (Zhan et al ).…”

“…H. Zhang, Zhang et al (), H. Zhang, Zhao et al (), and X. Zhang et al () reported a strategy for mass production of mitotic gynogenetic doubled haploids (DHs) in the Japanese flounder, P. olivaceus . They compared the results of mitotic gynogenetic DHs induced from four females of different genetic backgrounds, including a wild‐type female, a meiotic gynogenetic female, a heterozygous mitotic gynogenetic female, and a heterozygous clonal female.…”

“…The heterozygous clonal female group had the highest hatching rate, lowest abnormality rate, and highest homozygosity rate. They hypothesized that high homozygosity indicated fewer deleterious recessive genes, and therefore, the mortality of the DH progeny from homozygous deleterious mutations might decline, resulting in high survival rate (H. Zhang, Zhang et al ; H. Zhang, Zhao et al ; X. Zhang et al ). This strategy of successful mass production of DHs could help in research and breeding programs in the Japanese flounder.…”

“…Other papers in this special issue are focused on the characterization of important genes potentially affecting aquaculture performance in various organisms (Jin et al ; Ping et al ; Shan et al ; H. Zhang, Zhang et al ; H. Zhang, Zhao et al ; X. Zhang et al ). Shan et al () cloned the crustacean cardioactive peptide (CCAP) gene from eyestalks of the oriental river prawn, Macrobrachium nipponense , and studied its expression profile under hypoxic conditions.…”