Characterization and Identification of Isolated Microorganism from Agricultural Soil for Degradation of Cellulose Based Waste

Abdullah, Nur Syazrin Amalina; Manaf, Shareena Fairuz Abdul; Aziz, Nur Shahidah Ab; Hamzah, Fazlena; Idris, Norazlina

doi:10.4028/www.scientific.net/amr.1113.230

Cited by 2 publications

(2 citation statements)

References 7 publications

(10 reference statements)

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“…has the ability to reduce oxidative damages by radicals. Furthermore, another study revealed that the extract of Micrococcus lutues containing hexahydro- (2) and pyrrolo[1,2a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)- (3) exhibited promising cytotoxic effect on HCT15 with (Abdullah et al, 2015 ). Thus, it was suggested that both of the identified pyrrolopyrazine could have contributed the antioxidant and anticancer activities observed in MUM256 extract.…”

Section: Resultsmentioning

confidence: 99%

Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil

et al. 2015

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A Streptomyces strain, MUM256 was isolated from Tanjung Lumpur mangrove soil in Malaysia. Characterization of the strain showed that it has properties consistent with those of the members of the genus Streptomyces. In order to explore the potential bioactivities, extract of the fermented broth culture of MUM256 was prepared with organic solvent extraction method. DPPH and SOD activity were utilized to examine the antioxidant capacity and the results have revealed the potency of MUM256 in superoxide anion scavenging activity in dose-dependent manner. The cytotoxicity of MUM256 extract was determined using cell viability assay against 8 different panels of human cancer cell lines. Among all the tested cancer cells, HCT116 was the most sensitive toward the extract treatment. At the highest concentration of tested extract, the result showed 2.3-, 2.0-, and 1.8-folds higher inhibitory effect against HCT116, HT29, and Caco-2 respectively when compared to normal cell line. This result has demonstrated that MUM256 extract was selectively cytotoxic toward colon cancer cell lines. In order to determine the constituents responsible for its bioactivities, the extract was then subjected to chemical analysis using GC-MS. The analysis resulted in the identification of chemical constituents including phenolic and pyrrolopyrazine compounds which may responsible for antioxidant and anticancer activities observed. Based on the findings of this study, the presence of bioactive constituents in MUM256 extract could be a potential source for the development of antioxidative and chemopreventive agents.

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Section: Resultsmentioning

confidence: 99%

Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil

et al. 2015

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“…Microscopic examination of the slides was done with a microscope using the oil immersion (×100) lens. Biochemical tests such as indole, hydrogen sulphide, catalase, citrate, methyl red, and sugar fermentation tests were also performed according to established procedures [31,32].…”

Section: Identification Of Isolatesmentioning

confidence: 99%

Occurrence of Antibiotics and Antibiotic-Resistant Bacteria in Landfill Sites in Kumasi, Ghana

Borquaye

Ekuadzi

Darko

et al. 2019

Journal of Chemistry

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The incidence of antimicrobial resistance among microbial communities is a major threat to global health care and security. Landfills, which are reservoirs for many pharmaceuticals, provide a conducive habitat for antimicrobial-resistant microbes and resistant gene transfer and are therefore a major contributor to the phenomenon of antimicrobial resistance. Hence, this study determined the levels of three widely used antibiotics, metronidazole, penicillin, and amoxicillin, and the occurrence of antimicrobial resistance amongst microbes in soil and leachate samples from active and abandoned landfill sites in Kumasi, Ghana. Soil samples were collected from one active and four abandoned landfills, while leachate specimen was collected only from the active landfill. Sonication and solid-phase extraction (SPE) were used for sample preparation, followed by analysis via an HPLC-PDA method. Isolation and characterization of bacteria were done using standard bacteriological techniques. Antibiotic susceptibility testing was determined following the European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. Antibiotics were detected at very high concentrations in the specimen collected from both active and abandoned landfill sites. For leachate samples obtained from Dompoase, penicillin was present at the highest concentration (67.42 ± 5.35 μg/mL, p<0.05) followed by metronidazole (18.25 ± 7.92 μg/mL) and amoxicillin (10.96 ± 6.93 μg/mL). In general, the levels of antibiotics in soil samples were similar at both active and abandoned landfill sites. Nonetheless, as with leachates, penicillin levels were much higher (p<0.05) than levels of amoxicillin and metronidazole within any particular site. When screened against some antibiotics, Enterobacteriaceae and some Bacillus and Listeria species isolated from the soil and leachate samples proved to be resistant. The high levels of antibiotics coupled with the presence of resistant microbes at these landfills sites call for immediate measures to halt the disposal of pharmaceuticals in the environment so as to avert any possible public health setback.

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Characterization and Identification of Isolated Microorganism from Agricultural Soil for Degradation of Cellulose Based Waste

Cited by 2 publications

References 7 publications

Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil

Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil

Occurrence of Antibiotics and Antibiotic-Resistant Bacteria in Landfill Sites in Kumasi, Ghana

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