Characterization and Identification of Differentially Expressed Genes Involved in Thermal Adaptation of the Hong Kong Oyster Crassostrea hongkongensis by Digital Gene Expression Profiling

Li, Jun; Zhang, Yuehuan; Mao, Fan; Tong, Ying; Liu, Ying; Zhang, Yang; Zhou, Yu

doi:10.3389/fmars.2017.00112

Cited by 24 publications

(11 citation statements)

References 64 publications

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“…HSP70 plays several essential roles in cellular protein metabolism [110,111] while HSP40 facilitates cellular recovery from adverse effects of damaged or misfolded proteins (proteotoxic stress). Changes in HSPs, in addition to up/down regulation of HSP40, HSP70 and HSP90 have typically been reported in response to thermal stress in bivalves [95,112,113] and other environmental contaminants such as B[a]P [33]. In general, the consistent enrichment of genes involved in the endoplasmic-reticulum associated protein degradation (ERAD) pathway suggest that aqueous fullerene exposure targets the cellular pathway involved in targeting misfolding proteins for ubiquitination (post-translational modification) and subsequent degradation by proteasomes (protein degrading complex, breaks peptide bonds).…”

Section: Resultsmentioning

confidence: 99%

Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels

et al. 2019

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This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by Gas Chromatography–Mass Spectrometry (GC–MS), Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) and Inductively Coupled Plasma Mass Spectrometer (ICP–MS). Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the interactive effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were downregulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.

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Section: Resultsmentioning

confidence: 99%

Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels

et al. 2019

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“…It is interesting to note the overlap between organismal response to fullerene exposure and that of organismal response to thermal stress. Observed enrichment pathways in the current study viz protein processing in endoplasmic reticulum, apoptosis, ubiquitin mediated proteolysis, endocytosis, spliceosome, and MAPK signalling pathway have been observed as differentially enriched in oysters as a response to thermal stress[114].…”

mentioning

confidence: 53%

“…HSP70 plays several essential roles in cellular protein metabolism [112,113] while HSP40 facilitates cellular recovery from adverse effects of damaged or misfolded proteins (proteotoxic stress). Changes in HSPs, in addition to up/down regulation of HSP40, HSP70 and HSP90 have typically been reported in response to thermal stress in bivalves [98,114,115]…”

Section: Protein Expression Profilesmentioning

confidence: 99%

Antagonistic Interactions between Benzo[a]pyrene and C<sub>60</sub> in Toxicological Response of Marine Mussels

Barranger¹,

Langan²,

Sharma³

et al. 2019

Preprint

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This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.

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“…S8). These terms were associated with high pH exposure in the Antarctic pteropod (Johnson & Hofmann, 2016) and thermal response in the genus Crassostrea (Zhang et al, 2012, Li et al, 2017b). In M. galloprovincialis from the Mediterranean Sea (GalM) metal ion binding, purine ribonucleoside triphosphate binding, lipid transport and oxidative phosphorylation were enriched (Fig.…”

Section: Discussionmentioning

confidence: 99%

Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes

Malachowicz¹,

Wenne²

2019

PeerJ

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In molluscs, the shell secreted by mantle tissue during the biomineralization process is the first barrier against predators and mechanical damage. Changing environmental conditions, such as ocean acidification, influence shell strength and thus protection of the soft body within. Mussels are marine bivalves with important commercial and ecological value worldwide. Despite this importance, the proteins involved in the biomineralization and pigmentation processes in Mytilus spp. remain unclear, as does taxonomy of Mytilus taxa, though there have been many molecular studies. To further understanding in these areas, this study aimed to characterize and compare mantle transcriptomes of four mussel taxa using next generation sequencing. Mussels representing four taxa, were collected from several localities and RNA from mantle tissue was extracted. RNA sequences obtained were assembled, annotated and potential molecular markers, including simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) were identified. Candidate contigs putatively related to biomineralization and pigmentation processes were then selected and several transcripts were chosen for phylogenetic analyses from the Bivalvia class. Transcriptome comparisons between Mytilus taxa, including gene ontology (GO) enrichment analysis and orthologues identification were performed. Of assembled contigs, 46.57%, 37.28% and 17.53% were annotated using NCBI NR, GO and Kyoto Encyclopedia of Genes and Genomes databases, respectively. Potential SSRs (483) and SNPs (1,497) were identified. Results presented a total of 1,292 contigs putatively involved in biomineralization and melanogenesis. Phylogenetic analyses of α-carbonic anhydrase, chitinase and tyrosinase revealed complex evolutionary history and diversity of these genes, which may be a result of duplication events or adaptation to different environments in mussels and other bivalves. Enrichment analyses revealed GO terms associated with pH and thermal response in Mytilus edulis from the North Sea and M. galloprovincialis from the Mediterranean Sea. The phylogenetic analysis within the genus Mytilus revealed M. californianus and M. coruscus to be genetically more distant from the other taxa: M. trossulus, M. edulis, M. chilensis and M. galloprovincialis. This work represents the first mantle transcriptome comparison between Mytilus taxa and provides contigs putatively involved in biomineralization.

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Characterization and Identification of Differentially Expressed Genes Involved in Thermal Adaptation of the Hong Kong Oyster Crassostrea hongkongensis by Digital Gene Expression Profiling

Cited by 24 publications

References 64 publications

Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels

Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels

Antagonistic Interactions between Benzo[a]pyrene and C<sub>60</sub> in Toxicological Response of Marine Mussels

Mantle transcriptome sequencing of Mytilus spp. and identification of putative biomineralization genes

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