Characterization and Identification of a Chymotryptic Hydrolysate of Alpha-Lactalbumin Stimulating Cholecystokinin Release in STC-1 Cells

Abstract

Alpha-lactalbumin hydrolysate is of significant interest, due to its potential application as a source of bioactive peptides in nutraceutical and pharmaceutical domains. This study was focused on the cholecystokinin (CCK) family compounds which are small peptides involved in the satiety control. The action of chymotryptic hydrolysate of alpha-lactalbumin on cholecystokinin release from intestinal endocrine STC-1 cells was investigated. We demonstrated for the first time that a chymotryptic hydrolysate of alpha-lactalbumin was able to highly stimulate CCK-releasing activity from STC-1 cells. The peptidic hydrolysate was characterized by LC/MS and MS/MS, thus highlighting the presence of 11 fractions containing 21 peptides, each potentially having the desired activity.

Bioactive peptides derived from natural proteins with respect to diversity of their receptors and physiological effects

“…It has been suggested that the receptor of GLF might be common to that of complement C1q [30]. Peptides stimulating the release of cholecystokinin have been reported to be released from -lactalbumin [31].…”

Identification of pyrroloindoline-containing cyclic hexapeptides in the metabolites of Streptomyces alboflavus 313 by HPLC-DAD-ESI-MS/MS

“…In recent years, this hybrid technique has also been used for the fast identification of natural peptides. [13][14][15][16][17] To investigate the chemical biodiversity of biologically active cyclic hexapeptides in the metabolites from microorganism, we reported here the identification of pyrroloindoline-containing cyclic hexapeptides in the fermentation broth of S. alboflavus 313 by HPLC-diode array detector (DAD)-ESI-MS/MS. All NW-series cyclic hexapeptides consist of N-methylalanine (or alanine), valine (or leucine), chlorinated (or non-chlorinated) tryptophan derivative (Trp) and three piperazic acids (Pas; or its derivatives), their structures are shown in Figure 2.…”