Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida

Zhong, Qi-Wu; Zhou, Tingwei; Qiu, Wenhui; Wang, Yakun; Xu, Qiao-Li; Ke, Songze; Wang, Sijia; Jin, Weihua; Chen, Jianwei; Zhang, Huawei; Wei, Bin; Wei, Bin

doi:10.1016/j.foodchem.2020.128148

Cited by 62 publications

(22 citation statements)

References 40 publications

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“…Our results showed that the supplement of the high-dose NNP-2 polysaccharides (200 µg/mL) with inulin stimulated an increase in the gene expression of the IRS1/PI3K/Akt pathway in HepG2 cells compared to the observations in the inulin group. A previous study demonstrated that some polysaccharides, such as Sargassum fusiforme polysaccharide [ 52 ] and Morchella esculenta FMP-1 polysaccharide [ 53 ], can affect the IRS1/PI3K/AKT signaling pathway, while sulfated polysaccharides derived from brown seaweed Undaria pinnatifida have no effect on this pathway [ 54 ].…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Anh

Yang

2021

Polymers

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Polysaccharides are key bioactive compounds in lotus plumule tea, but their anti-diabetes activities remain unclear. The purpose of this study was to investigate the prebiotic activities of a novel polysaccharide fraction from the Nelumbo nucifera lotus plumule, and to examine its regulation of glucose metabolism in insulin-resistant HepG2 cells. The N. nucifera polysaccharide (NNP) was purified after discoloration, hot water extraction, ethanol precipitation, and DEAE-cellulose chromatography to obtain purified polysaccharide fractions (NNP-2). Fourier transform infrared spectroscopy was used to analyze the main structural characteristics and functional group of NNP-2. Physicochemical characterization indicated that NNP-2 had a molecular weight of 110.47 kDa and consisted of xylose, glucose, fructose, galactose, and fucose in a molar ratio of 33.4:25.7:22.0:10.5:8.1. The prebiotic activity of NNP-2 was demonstrated in vitro using Lactobacillus and Bifidobacterium. Furthermore, NNP-2 showed bioactivity against α-glucosidase (IC50 = 97.32 µg/mL). High glucose-induced insulin-resistant HepG2 cells were used to study the effect of NNP-2 on glucose consumption, and the molecular mechanism of the insulin transduction pathway was studied using RT-qPCR. NNP-2 could improve insulin resistance by modulating the IRS1/PI3K/Akt pathway in insulin-resistant HepG2 cells. Our data demonstrated that the Nelumbo nucifera polysaccharides are potential sources for nutraceuticals, and we propose functional food developments from the bioactive polysaccharides of N. nucifera for the management of diabetes.

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Section: Discussionmentioning

confidence: 99%

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Anh

Yang

2021

Polymers

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“…The report pointed out that seaweed polysaccharide had the effect to regulate glucose and lipid metabolism, and show the physiological activities of improving the symptoms of diabetes and lipid metabolism ( Zhong et al, 2021 ). Certainly, researchers confirmed the effect of GLP on glucose and lipid metabolism.…”

Section: Physiological Activities Of Glpmentioning

confidence: 99%

Insights on preparation, structure and activities of Gracilaria lemaneiformis polysaccharide

Long

Liu

et al. 2021

Food Chemistry: X

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“…Repairing islet cells to increase insulin secretion and increasing glycogen synthesis to increase glucose utilization are beneficial to reducing blood glucose content. The MSPs from U. pinnatifida have a strong α-glucosidase inhibitory activity, which can improve glucose intake, reduce islet cell damage, and promote liver glycogen synthesis [48].…”

Section: Regulating Glucose Metabolismmentioning

confidence: 99%

Marine Sulfated Polysaccharides: Preventive and Therapeutic Effects on Metabolic Syndrome: A Review

et al. 2021

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Metabolic syndrome is the pathological basis of cardiovascular and cerebrovascular diseases and type 2 diabetes. With the prevalence of modern lifestyles, the incidence of metabolic syndrome has risen rapidly. In recent years, marine sulfate polysaccharides (MSPs) have shown positive effects in the prevention and treatment of metabolic syndrome, and they mainly come from seaweeds and marine animals. MSPs are rich in sulfate and have stronger biological activity compared with terrestrial polysaccharides. MSPs can alleviate metabolic syndrome by regulating glucose metabolism and lipid metabolism. In addition, MSPs prevent and treat metabolic syndrome by interacting with gut microbiota. MSPs can be degraded by gut microbes to produce metabolites such as short chain fatty acids (SCFAs) and free sulfate and affect the composition of gut microbiota. The difference between MSPs and other polysaccharides lies in the sulfation pattern and sulfate content, therefore, which is very important for anti-metabolic syndrome activity of MSPs. This review summarizes the latest findings on effects of MSPs on metabolic syndrome, mechanisms of MSPs in treatment/prevention of metabolic syndrome, interactions between MSPs and gut microbiota, and the role of sulfate group and sulfation pattern in MSPs activity. However, more clinical trials are needed to confirm the potential preventive and therapeutic effects on human body. It may be a better choice to develop new functional foods containing MSPs for dietary intervention in metabolic syndrome.

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Characterization and hypoglycemic effects of sulfated polysaccharides derived from brown seaweed Undaria pinnatifida

Cited by 62 publications

References 40 publications

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Polysaccharide Derived from Nelumbo nucifera Lotus Plumule Shows Potential Prebiotic Activity and Ameliorates Insulin Resistance in HepG2 Cells

Insights on preparation, structure and activities of Gracilaria lemaneiformis polysaccharide

Marine Sulfated Polysaccharides: Preventive and Therapeutic Effects on Metabolic Syndrome: A Review

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