Characterization and gonadal expression of FOXL2 relative to Cyp19a genes in spotted scat Scatophagus argus

Liu, Huifen; Mu, Xingjiang; Liu, Gui; Su, Maoliang; Li, Hong; Guang, Zhang; Liu, Zhenhao; Zhang, Junbin

doi:10.1016/j.gene.2014.12.060

Cited by 35 publications

(19 citation statements)

References 41 publications

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“…It is worth noting that foxl2/3 mRNA and Foxl2/3 proteins are con-stantly expressed in the developing and adult testis reinforcing a possible role of foxl2/3 in this tissue [Liu et al, 2015a] ( table 1 ).…”

Section: Teleost Fish Speciesmentioning

confidence: 88%

Foxl2 and Its Relatives Are Evolutionary Conserved Players in Gonadal Sex Differentiation

Bertho

Pasquier

Pan

et al. 2016

Sex Dev

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Foxl2 is a member of the large family of Forkhead Box (Fox) domain transcription factors. It emerged during the last 15 years as a key player in ovarian differentiation and oogenesis in vertebrates and especially mammals. This review focuses on Foxl2 genes in light of recent findings on their evolution, expression, and implication in sex differentiation in animals in general. Homologs of Foxl2 and its paralog Foxl3 are found in all metazoans, but their gene evolution is complex, with multiple gains and losses following successive whole genome duplication events in vertebrates. This review aims to decipher the evolutionary forces that drove Foxl2/3 gene specialization through sub- and neo-functionalization during evolution. Expression data in metazoans suggests that Foxl2/3 progressively acquired a role in both somatic and germ cell gonad differentiation and that a certain degree of sub-functionalization occurred after its duplication in vertebrates. This generated a scenario where Foxl2 is predominantly expressed in ovarian somatic cells and Foxl3 in male germ cells. To support this hypothesis, we provide original results showing that in the pea aphid (insects) foxl2/3 is predominantly expressed in sexual females and showing that in bovine ovaries FOXL2 is specifically expressed in granulosa cells. Overall, current results suggest that Foxl2 and Foxl3 are evolutionarily conserved players involved in somatic and germinal differentiation of gonadal sex.

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Section: Teleost Fish Speciesmentioning

confidence: 88%

Foxl2 and Its Relatives Are Evolutionary Conserved Players in Gonadal Sex Differentiation

Bertho

Pasquier

Pan

et al. 2016

Sex Dev

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“…Several studies in mammals and fish have found that Foxl2 was expressed at its highest levels in the ovary and played a crucial role in the regulation of the early development of the female gonad (Oshima et al ; Amine et al ; Jiang et al ; Sandrine et al ; Ashida et al ; Berta et al ). Only limited studies reported that Foxl2 mRNA was expressed in testis (Wang et al ; Oshima et al ; Liu et al ). Mn‐Foxl2 mRNA was expressed in all detected tissues in M. nipponense , implying an important biological function.…”

Section: Discussionmentioning

confidence: 99%

“…In Rana rugose , Foxl2 mRNA expression remained at a high level at the ovarian reproductive cycle (Oshima et al ). In Scatophagus argus , Foxl2 mRNA expression decreased as gametogenesis proceeded and reached the lowest level at the mature stage in both testis and ovary (Liu et al ). The results in this study were consistent with that in S. argus , indicating that Mn‐Foxl2 plays essential roles in stimulating early gonad development.…”

Section: Discussionmentioning

confidence: 99%

“…In situ hybridization of Foxl2 has been performed in some aquaculture species. The expression of Foxl2 was mainly localized in granulosa cells and somatic cells surrounding oocytes in the ovary (Wang et al ; Oshima et al ; Ashida et al ; Liu et al ), while strong expression of Foxl2 gene was observed in the interstitial cells of testes, including tubules and Leydig cells (Liu et al ). In this study, strong signals were observed in the nucleolus, indicating that Foxl2 promotes the nucleolus development in M. nipponense ovary.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Cloning, Expression, and In Situ Hybridization Analysis of Forkhead Box Protein L2 during Development in Macrobrachium nipponense

Jin

Jiang

et al. 2018

J World Aquaculture Soc

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Forkhead box protein L2 (Foxl2) has dramatic effects on early differentiation and development of the female gonad in mammals and fish through the repression of SOX9 expression. In this study, we aimed to isolate a full‐length cDNA sequence encoding Foxl2 from the Macrobrachium nipponense (Mn‐Foxl2) and investigated its gene function. The full‐length cDNA sequence of Mn‐Foxl2 was 2097 bp with an open reading frame of 1740 bp, encoding 579 amino acids. Phylogenetic analysis revealed considerable evolutionary divergence between Mn‐Foxl2 and Foxl2 from fish. Mn‐Foxl2 may be involved in gonadal sex differentiation, based on its dramatically high expression during the sex‐differentiation sensitive period. Tissue distributions indicated that Mn‐Foxl2 mRNA expression was higher in the testis than that in the ovary and higher in males than in females in the same tissues, implying that Foxl2 may promote both male and female sexual development in M. nipponense. In situ hybridization analysis and quantitative polymerase chain reaction analysis in the gonad during the reproductive cycle further confirmed the important roles of Foxl2 in early gonad development in M. nipponense. This study advances our understanding of the role of Foxl2 in M. nipponense and provides the basis for further studies of Foxl2 in crustaceans.

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“…However, in mice Foxl2 expression was also observed transiently and at very low levels in the XY gonad, although the protein was not detected [Auguste et al, 2011]. Since these first reports, the role of FOXL2 as a master regulator of ovarian differentiation has been abundantly described in various vertebrate species, such as fish [Baron et al, 2004;Nakamoto et al, 2006;Liu et al, 2007;Yamaguchi et al, 2007;Amberg et al, 2010;Hu et al, 2014;Liu H et al, 2015;Wang et al, 2015], amphibians [Oshima et al, 2008;Okada et al, 2009], reptiles [Rhen et al, 2007;Alam et al, 2008;Moore et al, 2010;Shoemaker-Daly et al, 2010], and birds [Govoroun et al, 2004]. More recently, FOXL2 ovarian expression was also reported in invertebrate species, such as crustaceans [Ma et al, 2012;Meng et al, 2015] and molluscs [Dheilly et al, 2012;Liu et al, 2012;Zhang et al, 2014;Shi et al, 2015].…”

Section: Identification Of Foxl2 As An Ovarian Factormentioning

confidence: 99%

Involvement of FOXL2 and RSPO1 in Ovarian Determination, Development, and Maintenance in Mammals

Pannetier

Chassot

Chaboissier

et al. 2016

Sex Dev

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In mammals, sex determination is a process through which the gonad is committed to differentiate into a testis or an ovary. This process relies on a delicate balance between genetic pathways that promote one fate and inhibit the other. Once the gonad is committed to the female pathway, ovarian differentiation begins and, depending on the species, is completed during gestation or shortly after birth. During this step, granulosa cell precursors, steroidogenic cells, and primordial germ cells start to express female-specific markers in a sex-dimorphic manner. The germ cells then arrest at prophase I of meiosis and, together with somatic cells, assemble into functional structures. This organization gives the ovary its definitive morphology and functionality during folliculogenesis. Until now, 2 main genetic cascades have been shown to be involved in female sex differentiation. The first is driven by FOXL2, a transcription factor that also plays a crucial role in folliculogenesis and ovarian fate maintenance in adults. The other operates through the WNT/CTNNB1 canonical pathway and is regulated primarily by R-spondin1. Here, we discuss the roles of FOXL2 and RSPO1/WNT/ CTNNB1 during ovarian development and homeostasis in different models, such as humans, goats, and rodents.

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Characterization and gonadal expression of FOXL2 relative to Cyp19a genes in spotted scat Scatophagus argus

Cited by 35 publications

References 41 publications

Foxl2 and Its Relatives Are Evolutionary Conserved Players in Gonadal Sex Differentiation

Foxl2 and Its Relatives Are Evolutionary Conserved Players in Gonadal Sex Differentiation

Molecular Cloning, Expression, and In Situ Hybridization Analysis of Forkhead Box Protein L2 during Development in Macrobrachium nipponense

Involvement of FOXL2 and RSPO1 in Ovarian Determination, Development, and Maintenance in Mammals

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