Characterization and genomic study of “phiKMV-Like” phage PAXYB1 infecting Pseudomonas aeruginosa

Abstract

Bacteriophage PAXYB1 was recently isolated from wastewater samples. This phage was chosen based on its lytic properties against clinical isolates of Pseudomonas aeruginosa (P. aeruginosa). In the present study, characterized PAXYB1, clarified its morphological and lytic properties, and analyzed its complete genome sequence. Based on the morphology of PAXYB1, it is a Podoviridae. The linear GC-rich (62.29%) double-stranded DNA genome of PAXYB1 is 43,337 bp including direct terminal repeats (DTRs) of 468 bp. It contains 60 open reading frames (ORFs) that are all encoded within the same strand. We also showed that PAXYB1 is a virulent phage and a new member of the phiKMV-like phages genus. Twenty-eight out of sixty predicted gene products (gps) showed significant homology to proteins of known function, which were confirmed by analyzing the structural proteome. Altogether, our work identified a novel lytic bacteriophage that lyses P. aeruginosa PAO1 and efficiently infects and kills several clinical isolates of P. aeruginosa. This phage has potential for development as a biological disinfectant to control P. aeruginosa infections.

The Bacteriophage Pf-10—A Component of the Biopesticide “Multiphage” Used to Control Agricultural Crop Diseases Caused by Pseudomonas syringae

“…The adsorption constant of phage Pf-10 calculated using the equation described by Kropinski [45] is (3.59 ± 1.42) × 10 −9 mL/min. The adsorption rate of phage Pf-10 was similar to that of other reported Pseudomonas-infecting phages of the Autographiviridae family, namely: Pseudomonas phage Andromeda (1.48 × 10 −9 mL/min) [63] and Pseudomonas phage PAXYB1 (3.70 × 10 −9 mL/min) [64].…”

Shotgun Metagenomics evaluation of soil fertilization effect on the rhizosphere viral community of maize plants

“…PhiKMV-like viruses are lytic phages that infect Pseudomonas aeruginosa. They are members of the family Podoviridae with short noncontractile tails and double stranded DNA (Yu et al 2017). Another important Podoviridae viruses are T7like viruses.…”

Molecular Characteristics of Novel Phage vB_ShiP-A7 Infecting Multidrug-Resistant Shigella flexneri and Escherichia coli, and Its Bactericidal Effect in vitro and in vivo

“…Phage vB_ShiP-A7 was purified following the protocol of Yu et al (2017). Briefly, phage vB_ShiP-A7 was added to the earlylog-phase liquid culture of S. flexneri A7.…”

“…Five test tubes containing 10 9 phage particles were immersed in different temperature water baths for approximately 1 h (4, 25, 37, 45, and 50 • C). Thereafter, the phage titers of all the samples were determined using the double-layer agar method (Yu et al, 2017).Three independent repeated experiments were performed. The average value was used to generate the figure, and the standard error of the mean (SEM) was marked.…”