Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

Ho, Margaret T.; Li, Michelle S. M.; McDowell, Tim; MacDonald, Jacqueline; Yuan, Zhongshun

doi:10.1186/s12896-020-00632-z

Cited by 22 publications

(11 citation statements)

References 69 publications

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“…Hence both bacteria isolates were identified as potential hydrocarbon degraders that can utilize components in diesel oil as its carbon source. The present results are agreed with the finding of Ho, Li, McDowell, MacDonald and Yuan (2020), who observed the growth of bacteria on the minimal medium supplemented with 0.2% diesel as the only carbon source to assess their diesel-degrading properties. Carbon source is essential for building cells and important in various metabolic processes for bacteria to grow and multiply.…”

Section: Screening Of Hydrocarbon Degrading Bacteriasupporting

confidence: 93%

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

Arifin

Razali

Vigneswari

2021

Squalen Bull. Marine Fisheries Postharvest Biotech.

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Marine contamination caused by anthropogenic activities has side effects and causes severe contamination to the environment. Polychaetes are benthic organisms that live in the sediment and can be a good indicator of sediment contamination by organic compounds. In this study, bacterial strains were isolated and identified from the gut of polychaete worm Marphysa moribidii and the potential of the bacteria was evaluated to degrade hydrocarbon compounds. The isolated bacteria were primary and secondary screened on Minimal Salt Media (MSM) agar supplemented with 1% v/v of diesel oil. Diesel degradation analysis was performed by inoculating potential bacterium into MSM broth with 1% v/v diesel oil and incubated at 37 oC for 20 days. Diesel degradation percentage was analyzed using the gravimetric method, while the bacteria cell densities were measured using the standard plate count method. Then, the selected isolates were identified based on their morphological characteristics and 16S rDNA sequences. As a result, two bacteria isolates coded as Isolate 6 and Isolate 8 were able to degrade diesel oil up to 52.29% and 39.24% after 20 days of incubation. The 16S rDNA sequence analysis revealed that it was identified as Bacillus sp. strain UMTFA1 (RB) and Staphylococcus kloosii strain UMTFA2 (RS). Our result showed that these strains have the potential in oil-degrading processes, which will provide new insight into bioremediation process and decrease environmental pollution in soil and water contaminated with hydrocarbons.

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Section: Screening Of Hydrocarbon Degrading Bacteriasupporting

confidence: 93%

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

Arifin

Razali

Vigneswari

2021

Squalen Bull. Marine Fisheries Postharvest Biotech.

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“…A. baumannii is abundant in nature, and can be acquired from water, soil and living organisms. Some strains of Acinetobacter are known to be associate in biodegradation of different number of pollutants such as Permethrin (Zhan et al 2018 ), phenanthrene, 3-chloroaniline (Duc 2016 ), diesel (Ho et al 2020 ), indole (Sadauskas et al 2017 ), malathion (Xie et al 2009 ), deltamethrin (Tang et al 2020 ), azo dye (Sreedharan et al 2021 ) and phenol (Liu et al 2020 ). Biodegradation of cellulose, chitin, pectin, fluoranthene, and phenanthrene by Actinetobacter from deep Sea Sediments were reported by (Chen et al 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone

Shaker

Nagy

Adly

et al. 2022

World J Microbiol Biotechnol

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Ciprofloxacin (CIP) and levofloxacin (LEV), widely used fluoroquinolone antibiotics, are often found in sewage from the sewage treatment plants and marine environment. In this study, CIP and LEV biodegrading bacterial consortia were obtained from industrial wastewater. Microorganisms in these consortia were identified as Acinetobacter baumannii (A. baumannii), Klebsiella pneumoniae (K. pneumoniae) and Elizabethkingia miricola (E. miricola). The impacts of the critical operating parameters on the elimination of CIP and LEV by bacterial consortia have been investigated and optimized to achieve the maximum levels of CIP and LEV biodegradation. Using liquid chromatography with tandem mass spectrometry (LC-MS-MS), possible degradation pathways for CIP and LEV were suggested by analyzing the intermediate degradation products. The role of the enzymes fluoroquinolone-acetylating aminoglycoside (6′-N-acetyltransferase) and cytochrome P450 (CYP450) in the breakdown of fluoroquinolones (FQs) was investigated as well. According to our findings, various biodegradation mechanisms have been suggested, including cleavage of piperazine ring, substitution of F atom, hydroxylation, decarboxylation, and acetylation, as the main biotransformation reactions. This study discovers the ability of non-reported bacterial strains to biodegrade both CIP and LEV as a sole carbon source, providing new insights into the biodegradation of CIP and LEV. Graphical abstract

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“…The Cd(II) concentration in the supernatant was investigated by ICP-MS (Agilent Technologies 7800 ICP-MS) analysis [ 28 ]. Quantitative PCR (qPCR) was used to quantify the ratio of DNA levels of the specific estG / xcpR genes to determine the ratios of ZM05 to ZM03 in the coculture system under Cd(II) stress [ 29 , 30 ]. The primers used in qPCR are listed in Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

Acinetobacter tandoii ZM06 Assists Glutamicibacter nicotianae ZM05 in Resisting Cadmium Pressure to Preserve Dipropyl Phthalate Biodegradation

Wang¹,

Shen²,

Wu³

et al. 2021

Microorganisms

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Dipropyl phthalate (DPrP) coexists with cadmium as cocontaminants in environmental media. A coculture system including the DPrP-degrading bacterium Glutamicibacter nicotianae ZM05 and the nondegrading bacterium Acinetobacter tandoii ZM06 was artificially established to degrade DPrP under Cd(II) stress. Strain ZM06 relieved the pressure of cadmium on strain ZM05 and accelerated DPrP degradation in the following three ways: first, strain ZM06 adsorbed Cd(II) on the cell surface (as observed by scanning electron microscopy) to decrease the concentration of Cd(II) in the coculture system; second, the downstream metabolites of ZM05 were utilized by strain ZM06 to reduce metabolite inhibition; and third, strain ZM06 supplied amino acids and fatty acids to strain ZM05 to relieve stress during DPrP degradation, which was demonstrated by comparative transcriptomic analysis. This study provides an elementary understanding of how microbial consortia improve the degradation efficiency of organic pollutants under heavy metals contamination.

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Characterization and genomic analysis of a diesel-degrading bacterium, Acinetobacter calcoaceticus CA16, isolated from Canadian soil

Cited by 22 publications

References 69 publications

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

Isolation and Identification of Hydrocarbon-Degrading Bacteria from Polychaete Marphysa moribidii

Acinetobacter baumannii, Klebsiella pneumoniae and Elizabethkingia miricola isolated from wastewater have biodegradable activity against fluoroquinolone

Acinetobacter tandoii ZM06 Assists Glutamicibacter nicotianae ZM05 in Resisting Cadmium Pressure to Preserve Dipropyl Phthalate Biodegradation

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