Characterization and Genomic Analysis of Novel Vibrio parahaemolyticus Phage vB_VpaP_DE10

Abstract

In the present study, a novel lytic Vibrio parahaemolyticus phage, vB_VpaP_DE10, was isolated from sewage samples collected in Guangzhou city, China. Transmission electron microscopy revealed that phage vB_VpaP_DE10 has an icosahedral head (52.4 ± 2.5 nm) and a short non-contracted tail (21.9 ± 1.0 nm). Phage vB_VpaP_DE10 lysed approximately 31% (8/26) of the antibiotic-resistant V. parahaemolyticus strains tested. A one-step growth curve showed that phage vB_VpaP_DE10 has a relatively long latency time of 25 min and a burst size of ~19 PFU per cell. The genome of phage vB_VpaP_DE10 is a 42,871-bp-long dsDNA molecule with a G + C content of 49.19% and is predicted to contain 46 open reading frames, 26 of which are predicted to be related to functions such as phage structure, packaging, host lysis, and DNA metabolism. Sequence comparisons suggested that vB_VpaP_DE10 is a member of the genus Maculvirus within the family Autographiviridae. Morphological and genomic analysis indicated that vB_VpaP_DE10 is a novel V. parahaemolyticus phage.

Characterization and Preliminary Application of a Novel Lytic Vibrio parahaemolyticus Bacteriophage vB_VpaP_SJSY21

“…The infection efficiency of phage SJSY21 is remarkably high, with an optimal infection complex of only 0.01. Phage SJSY21 demonstrates a substantial lysis capacity, reaching 508, surpassing the V. parahaemolyticus bacteriophage vB_VpaP_DE10 reported by Ye et al [16]. Additionally, phage SJSY21 does not interact with several common probiotics, suggesting its potential for pathogen control by making mixtures with those probiotics.…”

“…The database used for sequence alignment was the NCBI NR database. Diamond blast was used to annotate protein-coding genes with eggNOG (http://eggnog6.embl.de/), and KAAS annotation (https://www.genome.jp/tools/kaas/) and InterPro software (http: //www.ebi.ac.uk/interpro/) were used to complete KEGG annotation and GO annotation analysis [15][16][17][18].…”

Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family

“…The latent period of phage XM1 was about 40 min, and its burst size was approximately 264 PFU/cell (Figure 2A). Previously, phage vB_VPAP_DE10 infecting V. parahaemolyticus has been shown to have a latent period of approximately 0-25 min, with a burst size of 19 PFU/cell [32]. Phages F23s2 and H256D1 showed a latent period of 0-20 min and 0-5 min, respectively, with a burst size of 12 PFU/cell and 131 PFU/cell, respectively [33].…”

Bacteriophage and non-pathogenic Vibrio to control diseases in shrimp aquaculture

“…Added the supernatant, containing the bacteriophage, to Vibrio parahaemolyticus cultures in 5 ml of TSBS [ 47 ]. The mixture was incubated at 28 °C for 8–12 h, during which the bacteriophages in the supernatant infected and reproduced within the Vibrio parahaemolyticus bacteria.…”