Characterization and genomic analysis of a broad-spectrum lytic phage HZ2201 and its antibiofilm efficacy against Pseudomonas aeruginosa

Characterization of the novel broad-spectrum lytic phage Phage_Pae01 and its antibiofilm efficacy against Pseudomonas aeruginosa

“…After incubation at different temperatures for 1 h, phage Phage_Pae01 still maintained high activity at a temperature of up to 60°C, while phage phiPA1-3 had a survival rate of about 40% at a temperature of 50°C. The activity of phage HZ2201 also decreased with the increase of temperature ( Fei et al, 2023 ; Tsai et al, 2023 ). It can be seen that phage Phage_Pae01 can still maintain a high activity state at a higher temperature.…”

“…Tsai et al isolated the phage phiPA1-3 of P. aeruginosa , which exhibited a lysis rate of 20% ( Tsai et al, 2023 ). Fei et al isolated a broad-spectrum P. aeruginosa phage HZ2201 with a cleavage rate of 78.38% ( Fei et al, 2023 ). It shows that Phage_Pae01 has a wide cleavage spectrum, overcomes the problem of narrow host spectrum in phage therapy, and can be used as a candidate for phage therapy.…”

Clinical pharmacists’ involvement in carbapenem antibiotics management at Wenzhou Integrated Hospital

“…Therefore, hospitals should strictly manage carbapenem use. Clinical pharmacists play an essential role in the management of these drugs by participating in ward rounds, consultations, and critical care analyses[ 12 ]. They can leverage their extensive theoretical knowledge and practical experience to analyze each patient’s specific condition and organ function as well as the characteristics of antimicrobial drugs.…”