Characterization and Genomic Analyses of Pseudomonas aeruginosa Podovirus TC6: Establishment of Genus Pa11virus

Tang, Chaofei; Deng, Chuanjiang; Zhang, Yi; Xiao, Cong; Wang, Jing; Rao, Xiancai; Hu, Fuquan; Lu, Shuguang

doi:10.3389/fmicb.2018.02561

Cited by 12 publications

(13 citation statements)

References 63 publications

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“…Based on a BLAST analysis against the NCBI standard nucleotide database, a total of 177 genes were predicted ( Table S1 ), and nine genes encoding tRNAs (tRNA-Val, tRNA-Gln, tRNA-Arg, tRNA-Glu, tRNA-Ile, tRNA-Pro, tRNA-Leu, tRNA-Cys, and tRNA-Gly) were detected. A BLASTp analysis revealed that 166 proteins had homologs in other phages or bacteria; however, most of these proteins were assigned as hypothetical proteins, and no homologs of phage integrases, excisionases, repressors, or transposases were predicted which suggested that phage PN09 is a lytic phage [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Characterization of a Lytic Bacteriophage against Pseudomonas syringae pv. actinidiae and Its Endolysin

Wang

Deng

et al. 2021

Viruses

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Pseudomonas syringae pv. actinidiae (Psa) is a phytopathogen that causes canker in kiwifruit. Few conventional control methods are effective against this bacterium. Therefore, alternative approaches, such as phage therapy are warranted. In this study, a lytic bacteriophage (PN09) of Psa was isolated from surface water collected from a river in Hangzhou, China in 2019. Morphologically, PN09 was classified into the Myoviridae family, and could lyse all 29 Psa biovar 3 strains. The optimal temperature and pH ranges for PN09 activity were determined as 25 to 35 °C and 6.0 to 9.0, respectively. The complete genome of PN09 was found to be composed of a linear 99,229 bp double-stranded DNA genome with a GC content of 48.16%. The PN09 endolysin (LysPN09) was expressed in vitro and characterized. LysPN09 was predicted to belong to the Muraidase superfamily domain and showed lytic activity against the outer-membrane-permeabilized Psa strains. The lytic activity of LysPN09 was optimal over temperature and pH ranges of 25 to 40 °C and 6.0 to 8.0, respectively. When recombinant endolysin LysPN09 was combined with EDTA, Psa strains were effectively damaged. All these characteristics demonstrate that the phage PN09 and its endolysin, LysPN09, are potential candidates for biocontrol of Psa in the kiwifruit industry.

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Section: Resultsmentioning

confidence: 99%

Characterization of a Lytic Bacteriophage against Pseudomonas syringae pv. actinidiae and Its Endolysin

Wang

Deng

et al. 2021

Viruses

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“…The P. aeruginosa phages TC6, O4, and IME180 showed significant relatedness and are very phylogenetically close to PA11. Therefore, these were classified as a new genus of phages infecting P. aeruginosa named Pa11virus ( Tang et al, 2018 ). Similarly, classifying PPAT and PPAY as Pa11virus contributed to the diversity and classification of Podoviridae .…”

Section: Discussionmentioning

confidence: 99%

“…The effect of this self-produced toxic protein must be considered while using such phases for biological therapy ( Watts, 2017 ). Pseudomonas phages GP100 and TC6 also contain hemagglutinin proteins ( Tang et al, 2018 ). This study isolated and identified two new virulent phage strains, which increases our understanding of the biodiversity of phages that can be used as antibacterial agents.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of two homolog phages infecting Pseudomonas aeruginosa

Niu

Xiaobo²,

Shang³

et al. 2022

Front. Microbiol.

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Bacteriophages (phages) are capable of infecting specific bacteria, and therefore can be used as a biological control agent to control bacteria-induced animal, plant, and human diseases. In this study, two homolog phages (named PPAY and PPAT) that infect Pseudomonas aeruginosa PAO1 were isolated and characterized. The results of the phage plaque assay showed that PPAT plaques were transparent dots, while the PPAY plaques were translucent dots with a halo. Transmission electron microscopy results showed that PPAT (65 nm) and PPAY (60 nm) strains are similar in size and have an icosahedral head and a short tail. Therefore, these belong to the short-tailed phage family Podoviridae. One-step growth curves revealed the latent period of 20 min and burst time of 30 min for PPAT and PPAY. The burst size of PPAT (953 PFUs/infected cell) was higher than that of PPAY (457 PFUs/infected cell). Also, the adsorption rate constant of PPAT (5.97 × 10−7 ml/min) was higher than that of PPAY (1.32 × 10−7 ml/min) at 5 min. Whole-genome sequencing of phages was carried out using the Illumina HiSeq platform. The genomes of PPAT and PPAY have 54,888 and 50,154 bp, respectively. Only 17 of the 352 predicted ORFs of PPAT could be matched to homologous genes of known function. Likewise, among the 351 predicted ORFs of PPAY, only 18 ORFs could be matched to genes of established functions. Homology and evolutionary analysis indicated that PPAT and PPAY are closely related to PA11. The presence of tail fiber proteins in PPAY but not in PPAT may have contributed to the halo effect of its plaque spots. In all, PPAT and PPAY, newly discovered P. aeruginosa phages, showed growth inhibitory effects on bacteria and can be used for research and clinical purposes.

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“…The isolated vB_KpnP_Klyazma phage has a morphology and genome structure typical of the Zobellviridae family [ 37 , 38 , 39 ]. Prior to being distinguished as the Zobellviridae family, such phages were grouped into the T7-like supergroup due to the virion morphology and homology of structural proteins [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of the First Zobellviridae Family Bacteriophage Infecting Klebsiella pneumoniae

Gorodnichev

Kornienko

Malakhova

et al. 2023

IJMS

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In order to address the upcoming crisis in the treatment of Klebsiella pneumoniae infections, caused by an increasing proportion of resistant isolates, new approaches to antimicrobial therapy must be developed. One approach would be to use (bacterio)phages and/or phage derivatives for therapy. In this study, we present a description of the first K. pneumoniae phage from the Zobellviridae family. The vB_KpnP_Klyazma podovirus, which forms translucent halos around the plaques, was isolated from river water. The phage genome is composed of 82 open reading frames, which are divided into two clusters located on opposite strands. Phylogenetic analysis revealed that the phage belongs to the Zobellviridae family, although its identity with the closest member of this family was not higher than 5%. The bacteriophage demonstrated lytic activity against all (n = 11) K. pneumoniae strains with the KL20 capsule type, but only the host strain was lysed effectively. The receptor-binding protein of the phage was identified as a polysaccharide depolymerase with a pectate lyase domain. The recombinant depolymerase protein showed concentration-dependent activity against all strains with the KL20 capsule type. The ability of a recombinant depolymerase to cleave bacterial capsular polysaccharides regardless of a phage’s ability to successfully infect a particular strain holds promise for the possibility of using depolymerases in antimicrobial therapy, even though they only make bacteria sensitive to environmental factors, rather than killing them directly.

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Characterization and Genomic Analyses of Pseudomonas aeruginosa Podovirus TC6: Establishment of Genus Pa11virus

Cited by 12 publications

References 63 publications

Characterization of a Lytic Bacteriophage against Pseudomonas syringae pv. actinidiae and Its Endolysin

Characterization of a Lytic Bacteriophage against Pseudomonas syringae pv. actinidiae and Its Endolysin

Isolation and characterization of two homolog phages infecting Pseudomonas aeruginosa

Isolation and Characterization of the First Zobellviridae Family Bacteriophage Infecting Klebsiella pneumoniae

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