Characterization and fungicide sensitivity of Colletotrichum godetiae causing sweet cherry fruit anthracnose in Guizhou, China

Abstract

Sweet cherry is an important fruit crop with high economic and ornamental value in China. However, cherry fruit anthracnose, caused by Colletotrichum species, greatly impacts cherry yield and quality. Here, we surveyed cherry anthracnose in Guizhou, China from 2019–2020. Necrotic sweet cherry fruits were collected from different areas in Guizhou and examined. A total of 116 Colletotrichum strains were isolated from these symptomatic fruits. Based on the morphological characteristics of the isolates and phylogenetic analyses of concatenate internal transcribed spacer (ITS) region and ACT, CHS-1, GAPDH, TUB2, and HIS3 genes, the pathogen responsible for causing sweet cherry anthracnose was identified as Colletotrichum godetiae. Pathogenicity tests were conducted by inoculating healthy sweet cherry fruits with spore suspensions of the fungal pathogen, and Koch’s postulates were confirmed by pathogen re-isolation and identification. The Q-1 isolate showed different sensitivities to 13 fungicides, exhibiting seven different modes of action, and its EC50 values ranged from 0.04 to 91.26 μg ml−1. According to that, the sensitivity of 20 isolates from different samples to ten fungicides with better performance, were measured. The results showed that 6 of the 10 fungicides (difenoconazole, propiconazole, prochloraz-manganese, pyraclostrobin, trifloxystrobin-tebuconazole, and difenoconazole-azoxystrobin) all showed higher sensitive to the 20\u00B0C. godetiae isolates, and no resistance groups appeared. Its EC50 values ranged from 0.013 to 1.563 μg ml−1. In summary, this is the first report demonstrating that C. godetiae causes sweet cherry anthracnose and the results of this study provide insights into how sweet cherry anthracnose could be effectively controlled in China.

Effects of Pre-Harvest Spraying with Salicylic Acid (SA) and Sodium Nitroprusside (SNP) on Storage Quality and Pathogenic Fungal Species in ‘Manaohong’ Cherries

“…anthracnose. Furthermore, inhibitory effects on anthracnose have been observed with difenoconazole, triclosan, and tebuconazole [54]. The pathogenic fungus identified in this study aligns with these findings, despite the different sweet cherry varieties examined.…”

“…In various regions of Guizhou Province, research has been conducted to isolate and identify decayed sweet cherry fruits, which has revealed Colletotrichum godetiae as the fungus responsible for sweet cherry anthracnose. Furthermore, inhibitory effects on anthracnose have been observed with difenoconazole, triclosan, and tebuconazole [54]. The pathogenic fungus identified in this study aligns with these findings, despite the different sweet cherry varieties examined.…”

Identification of Colletotrichum aenigma as the new causal agent of leaf blight disease on Aucuba japonica Thunb., and screenings of effective fungicides for its sustainable management

“…Moreover, difenoconazole exhibited the highest control efficiency (47.75%), indicating potent antifungal activity against Colletotrichum spp. ( Zhang et al, 2020 ; Peng et al, 2022 ). In contrast, mancozeb showed no potent inhibitory activity against C. aenigma BY827, with EC 50 values of 202.1461 μg/mL.…”

Occurrence and Characterization of Sclerotinia sclerotiorum Causing Fruit Rot on Sweet Cherry in Southern China

“…Sub-optimal cultivation regions located in southern China, such as Hangzhou, have subtropical monsoon climates, for which a rainy spring is one of the climate characteristics. Therefore, the high humidity and suitable temperature in spring lead to high incidences of various fungal diseases in the orchards, such as leaf spot, gummosis, shoot blight, anthracnose, blossom blight, and fruit rot, which seriously restrict the sustainable development of the sweet cherry industry in these regions [ 2 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ].…”