Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Kausar, Saima; Qian, Cen; Abbas, Muhammad Nadeem; Zhu, Bao Jian; Liu, Ya; Wang, Lei; Guo, Wenlei; Sun, Yu; Liu, Chao Liang

doi:10.14411/eje.2017.055

Cited by 11 publications

(2 citation statements)

References 44 publications

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“…The IMD and Toll signaling pathways and their related molecules are the primary components of the innate immune system, which regulate immune effectors, e.g., the production of antimicrobial proteins (Min et al, 2017;Kausar et al, 2017Kausar et al, , 2018. After the microbial invasion, Toll receptors detect microbial pathogens' components, thereby transfer signals to the D r a f t downstream molecules (Uematsu and Akira, 2006;Medzhitov et al, 2007;Kausar et al, 2019).…”

Section: Clarkiimentioning

confidence: 99%

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Huang

et al. 2021

Genome

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Aspartic proteinases are one of the four families of proteinase enzymes that are widely present in living organisms. They are involved in various physiological events, such as protein degradation, development, and host defense. However, the characterization and functional roles of aspartic proteinases remain to be elucidated in crustaceans. Here, we characterized a fragment of cathepsin D-like cDNA from red swamp crayfish, Procambarus clarkii (Pc-cathepsin D-like). The open reading frame of the Pc-cathepsin D-like gene contained 1152 bp, encoding a protein of 383 amino acid residues. We also evaluated the immunological role of the Pc-cathepsin D-like gene in vivo. Spatial distribution analysis revealed that the Pc-cathepsin D-like mRNA was high in the hepatopancreas, followed by the gut, gills, and hemocytes of P. clarkii. The expression levels of the Pc-cathepsin D-like gene increased following challenge with viral (polyinosinic: polycytidylic acid) and bacterial (lipopolysaccharides, peptidoglycan) PAMPs compared with PBS injection. The suppression of the Pc-cathepsin D-like gene by RNA interference significantly increased the expression of immune-associated genes. These results showed that the Pc-cathepsin D-like gene has an essential biological role in innate immune responses because it regulates the expression of immune-associated genes.

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Section: Clarkiimentioning

confidence: 99%

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Huang

et al. 2021

Genome

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“…To explore the role of Hh in insects in this work, the silkworm was chosen as a research model because this organism is agriculturally important for silk production and has been used extensively in pathological and genetic studies (Tanaka et al ., ; Kausar et al ., ). The midgut is one of the most important organs in the silkworm.…”

Section: Introductionmentioning

confidence: 97%

Hedgehog promotes cell proliferation in the midgut of silkworm, Bombyx mori

et al. 2019

Self Cite

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The Hedgehog (Hh) signaling pathway is one of the major regulators of embryonic development and tissue homeostasis in multicellular organisms. However, the role of this pathway in the silkworm, especially in the silkworm midgut, remains poorly understood. Here, we report that Bombyx mori Hedgehog (BmHh) is expressed in most tissues of silkworm larvae and that its functions are well-conserved throughout evolution. We further demonstrate that the messenger RNA of four Hh signaling components, BmHh ligand, BmPtch receptor, signal transducer BmSmo and transcription factor BmCi, are all upregulated following Escherichia coli or Bacillus thuringiensis infection, indicating the activation of the Hh pathway. Simultaneously, midgut cell proliferation is strongly promoted. Conversely, the repression of Hh signal transduction with double-stranded RNA or cyclopamine inhibits the expression of BmHh and BmCi and reduces cell proliferation. Overall, these findings provide new insights into the Hh signaling pathway in the silkworm, B. mori.

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Effects of supplementary astaxanthin in egg custard on antioxidant capacity and non-specific immunity during larvae metamorphosis of giant freshwater prawn (Macrobrachium rosenbergii)

Zhang

et al. 2023

Aquacult Int

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Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Cited by 11 publications

References 44 publications

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Molecular characterization and functional analysis of the lysosomal cathepsin D-like gene in red swamp crayfish, Procambarus clarkii

Hedgehog promotes cell proliferation in the midgut of silkworm, Bombyx mori

Effects of supplementary astaxanthin in egg custard on antioxidant capacity and non-specific immunity during larvae metamorphosis of giant freshwater prawn (Macrobrachium rosenbergii)

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