Characterization and functional analysis of GhWRKY42, a group IId WRKY gene, in upland cotton (Gossypium hirsutum L.)

Gu, Lijiao; Wei, Hengling; Wang, Hantao; Su, Junji; Yu, Shuang

doi:10.1186/s12863-018-0653-4

Cited by 33 publications

(26 citation statements)

References 59 publications

(54 reference statements)

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“…The WRKY TF is known as the largest superfamilies which participate in the regulation of proteins in plants [29]. In the past several years, growing evidence has shown that members of the WRKY gene family mainly participate in stress responses, plant growth and development and leaf senescence [30].…”

Section: Discussionmentioning

confidence: 99%

Overexpression of transcription factor SlWRKY28 improved the tolerance of Populus davidiana × P. bolleana to alkaline salt stress

et al. 2020

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Background WRKY transcription factors (TFs) have been suggested to play crucial roles in the response to biotic and abiotic stresses. This study is the first to report the alkaline salt regulation of the WRKY gene. Results In this study, we cloned a WRKY gene (SlWRKY28) from the Salix linearistipularis and then transferred to the Populus davidiana × P. bolleana for expression. Sequence analysis on the transcriptome of Salix linearistipular showed the significant up-regulation of WRKY gene expression in response to salt-alkali stress in seedlings. Our data showed that SlWRKY28 localized to the nucleus. Furthermore, the expression of the SlWRKY28 from female plants increased with saline-alkali stress according to the northern blot analysis results. The results of 3,3′-Diaminobenzidine (DAB) staining showed that hydrogen peroxide (H2O2) concentration was lower under stress, but ascorbate peroxidase (APX) enzyme activity was significantly higher in the overexpressed plants than that in non-transgenic (NT) plants. Conclusions We found out the SlWRKY28 induced regulation of the enzyme gene in the reactive oxygen species (ROS) scavenging pathway is a potential mechanism for transgenic lines to improve their resistance to alkaline salt. This study shows theoretical and practical significance in determining SlWRKY28 transcription factors involved in the regulation of alkaline salt tolerance.

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Section: Discussionmentioning

confidence: 99%

Overexpression of transcription factor SlWRKY28 improved the tolerance of Populus davidiana × P. bolleana to alkaline salt stress

et al. 2020

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“…The Gh_A11G1801 gene is corresponding to GhWRKY42 according to Dou et al ( 2014 ), and transgenic plants of its promoter, Gh_A11G1801p::GUS ( ProGhWRKY42 ::GUS), were obtained previously (Gu et al, 2018a ). To investigate promoter-induced GUS expression under stress conditions, transgenic Arabidopsis plants with foreign promoters were used for different treatments.…”

Section: Methodsmentioning

confidence: 99%

Identification, Expression, and Functional Analysis of the Group IId WRKY Subfamily in Upland Cotton (Gossypium hirsutum L.)

Wang

Wei

et al. 2018

Front. Plant Sci.

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WRKY transcription factors have diverse functions in regulating stress response, leaf senescence, and plant growth and development. However, knowledge of the group IId WRKY subfamily in cotton is largely absent. This study identified 34 group IId WRKY genes in the Gossypium hirsutum genome, and their genomic loci were investigated. Members clustered together in the phylogenetic tree had similar motif compositions and gene structural features, revealing similarity and conservation within group IId WRKY genes. During the evolutionary process, 14 duplicated genes appeared to undergo purification selection. Public RNA-seq data were used to examine the expression patterns of group IId WRKY genes in various tissues and under drought and salt stress conditions. Ten highly expressed genes were identified, and the ten candidate genes revealed distinct expression patterns under drought and salt treatments by qRT-PCR analysis. Among them, Gh_A11G1801 was used for functional characterization. GUS activity was differentially induced by various stresses in Gh_A11G1801p::GUS transgenic Arabidopsis plants. The virus-induced gene silencing (VIGS) of Gh_A11G1801 resulted in drought sensitivity in cotton plants, which was accompanied by elevated malondialdehyde (MDA) content and reduced catalase (CAT) content. Taken together, these findings obtained in this study provide valuable resources for further studying group IId WRKY genes in cotton. Our results also enrich the gene resources for the genetic improvements of cotton varieties that are suitable for growth in stressful conditions.

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“…Ancient gene transfer events were considered as the origin of these non-plant WRKYs based on distribution pattern analysis [11]. Numerous WRKY TFs have been experimentally revealed in various plant species, including (Arabidopsis thaliana) [12], sugarcane (Saccharum spontaneum) [13], barley (Hordeum vulgare) [14], rice (Oryza sativa) [15], physic nut (Jatropha curcas) [16], maize (Zea mays) [17], wheat (Triticum aestivum) [18], cotton (Gossypium hirsutum) [19] and so on (Table 1).…”

Section: Classification and Diversification Of Wrky Genes Familymentioning

confidence: 99%

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

Javed

Shabbir

Ali

et al. 2020

Plants

148

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Increasing vulnerability of crops to a wide range of abiotic and biotic stresses can have a marked influence on the growth and yield of major crops, especially sugarcane (Saccharum spp.). In response to various stresses, plants have evolved a variety of complex defense systems of signal perception and transduction networks. Transcription factors (TFs) that are activated by different pathways of signal transduction and can directly or indirectly combine with cis-acting elements to modulate the transcription efficiency of target genes, which play key regulators for crop genetic improvement. Over the past decade, significant progresses have been made in deciphering the role of plant TFs as key regulators of environmental responses in particular important cereal crops; however, a limited amount of studies have focused on sugarcane. This review summarizes the potential functions of major TF families, such as WRKY, NAC, MYB and AP2/ERF, in regulating gene expression in the response of plants to abiotic and biotic stresses, which provides important clues for the engineering of stress-tolerant cultivars in sugarcane.

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Characterization and functional analysis of GhWRKY42, a group IId WRKY gene, in upland cotton (Gossypium hirsutum L.)

Cited by 33 publications

References 59 publications

Overexpression of transcription factor SlWRKY28 improved the tolerance of Populus davidiana × P. bolleana to alkaline salt stress

Overexpression of transcription factor SlWRKY28 improved the tolerance of Populus davidiana × P. bolleana to alkaline salt stress

Identification, Expression, and Functional Analysis of the Group IId WRKY Subfamily in Upland Cotton (Gossypium hirsutum L.)

Transcription Factors in Plant Stress Responses: Challenges and Potential for Sugarcane Improvement

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