Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice

Tian, Xiaojie; Wang, Zhenyu; Li, Xiufeng; Lv, Tianxiao; Hua-zhao, Liu; Wang, Lizhi; Niu, Hongbin; Bu, Qingyun

doi:10.1186/s12284-015-0061-6

Cited by 141 publications

(123 citation statements)

References 21 publications

(50 reference statements)

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“…Moreover, OsPYL1 was down-regulated in OsDET1 RNAi plants, whereas OsPYL10 was upregulated (Table I). These results are consistent with previously reported OsPYL expression patterns after ABA treatment (Tian et al, 2015). Overall, these results indicate that the OsDET1 RNAi plants suffered from ABA stress, further supporting the notion that OsDET1 deficiency leads to ABA hypersensitivity.…”

Section: Transcriptome Analysis Of Osdet1 Rnai Plantssupporting

confidence: 92%

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

et al. 2016

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DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1. Here, we found that OsDET1 is essential for maintaining normal rice development. The repression of OsDET1 had detrimental effects on plant development, and leaded to contradictory phenotypes related to abscisic acid (ABA) in OsDET1 interference (RNAi) plants. We found that OsDET1 is involved in modulating ABA signaling in rice. OsDET1 RNAi plants exhibited an ABA hypersensitivity phenotype. Using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation assays, we determined that OsDET1 interacts physically with DAMAGED-SPECIFIC DNA-BINDING PROTEIN1 (OsDDB1) and CONSTITUTIVE PHOTOMORPHOGENIC10 (COP10); DET1-and DDB1-ASSOCIATED1 binds to the ABA receptors OsPYL5 and OsDDB1. We found that the degradation of OsPYL5 was delayed in OsDET1 RNAi plants. These findings suggest that OsDET1 deficiency disturbs the COP10-DET1-DDB1 complex, which is responsible for ABA receptor (OsPYL) degradation, eventually leading to ABA sensitivity in rice. Additionally, OsDET1 also modulated ABA biosynthesis, as ABA biosynthesis was inhibited in OsDET1 RNAi plants and promoted in OsDET1-overexpressing transgenic plants. In conclusion, our data suggest that OsDET1 plays an important role in maintaining normal development in rice and mediates the cross talk between ABA biosynthesis and ABA signaling pathways in rice.

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Section: Transcriptome Analysis Of Osdet1 Rnai Plantssupporting

confidence: 92%

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

et al. 2016

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“…Since the DRC reflected relative yield between drought stress and normal water condition, these six QTLs for DRC should be involved in the response to drought stress. Two known genes are close to the association intervals (Figure 3), i.e., OsPYL2 (Tian et al, 2015) near the lead SNP sf0207287189 and OsGA2ox9 (Lo et al, 2008) near the lead SNP sf0225263251.…”

Section: Resultsmentioning

confidence: 85%

“…These above results imply that OsGA2ox9 was likely affecting drought resistance via regulation of root growth in rice. OsPYL2 was reported to be an ABA receptor (Tian et al, 2015). ABA is involved in the adaptation of plants to abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study for Plant Height and Grain Yield in Rice under Contrasting Moisture Regimes

Fěng

Wei

et al. 2016

Front. Plant Sci.

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Drought is one of the vitally critical environmental stresses affecting both growth and yield potential in rice. Drought resistance is a complicated quantitative trait that is regulated by numerous small effect loci and hundreds of genes controlling various morphological and physiological responses to drought. For this study, 270 rice landraces and cultivars were analyzed for their drought resistance. This was done via determination of changes in plant height and grain yield under contrasting water regimes, followed by detailed identification of the underlying genetic architecture via genome-wide association study (GWAS). We controlled population structure by setting top two eigenvectors and combining kinship matrix for GWAS in this study. Eighteen, five, and six associated loci were identified for plant height, grain yield per plant, and drought resistant coefficient, respectively. Nine known functional genes were identified, including five for plant height (OsGA2ox3, OsGH3-2, sd-1, OsGNA1, and OsSAP11/OsDOG), two for grain yield per plant (OsCYP51G3 and OsRRMh) and two for drought resistant coefficient (OsPYL2 and OsGA2ox9), implying very reliable results. A previous study reported OsGNA1 to regulate root development, but this study reports additional controlling of both plant height and root length. Moreover, OsRLK5 is a new drought resistant candidate gene discovered in this study. OsRLK5 mutants showed faster water loss rates in detached leaves. This gene plays an important role in the positive regulation of yield-related traits under drought conditions. We furthermore discovered several new loci contributing to the three investigated traits (plant height, grain yield, and drought resistance). These associated loci and candidate genes significantly improve our knowledge of the genetic control of these traits in rice. In addition, many drought resistant cultivars screened in this study can be used as parental genotypes to improve drought resistance of rice by molecular breeding.

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“…Abscisic acid receptors cluster into three subfamilies (Ma et al ., ; Park et al ., ; Gonzalez‐Guzman et al ., ; Tian et al ., ; Gordon et al ., ; Lim and Lee, ) and share highly conserved elements among plant species, i.e. ABA binding residues, and gate and latch loops (Gonzalez‐Guzman et al ., ).…”

Section: Resultsmentioning

confidence: 98%

“…The discovery of ABA receptors in the eudicot model, Arabidopsis thaliana, opened the door for extensive ABA signaling research (Ma et al, 2009;Park et al, 2009). Subsequently, ABA receptors have been identified and characterized in various crops, such as tomato (Solanum lycopersicum), rice (Oryza sativa) and wheat (Triticum aestivum; Gonzalez-Guzman et al, 2014;Tian et al, 2015;Gordon et al, 2016). In addition, chemical screening of ABA agonists using A. thaliana receptors resulted in identification of small molecules that activate ABA signaling pathways across plant species (Okamoto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Non‐redundant functions of the dimeric ABA receptor BdPYL1 in the grass Brachypodium

et al. 2017

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Abiotic stresses have severe detrimental effects on agricultural productivity worldwide. Abscisic acid (ABA) levels rise in response to abiotic stresses, and play a role in coordinating physiological responses. ABA elicits its effects by binding a family of soluble receptors, increasing affinity of the receptors to type 2C phosphatases (PP2Cs) leading to phosphatase inhibition. In the current study, we conducted a comprehensive analysis of the ABA signaling pathway in the cereal model grass Brachypodium distachyon. The Brachypodium genome encodes a family of 10 functionally conserved ABA receptors. The 10th in the series, BdPYL10, encodes a defective receptor and is likely a pseudogene. Combinatorial protein interaction assay further validated computational analysis, which grouped Brachypodium ABA receptors into three subfamilies, similarly to Arabidopsis classification. Brachypodium subfamily III receptors inhibited PP2C activity in vitro and complemented Arabidopsis quadruple (pyr1/pyl1/pyl2/pyl4) mutant. BdPYL1 T-DNA mutant exhibited clear ABA hyposensitivity phenotypes during seedling establishment and in mature plants. Single receptor predominance is in agreement with high transcriptional abundance of only a small Brachypodium ABA receptors subset, harboring the higher marginal significance of BdPYL1. Our findings suggest that unlike the highly redundant ABA core signaling components of Arabidopsis, Brachypodium encompasses a more compact and specialized ABA receptor apparatus. This organization may contribute to plant adaptations to ecological niches. These results lay the groundwork for targeting the prominent ABA receptors for stress perception in grasses, and reveal functional differences and commonalities between monocots and eudicots.

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Characterization and Functional Analysis of Pyrabactin Resistance-Like Abscisic Acid Receptor Family in Rice

Cited by 141 publications

References 21 publications

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

The De-Etiolated 1 Homolog of Arabidopsis Modulates the ABA Signaling Pathway and ABA Biosynthesis in Rice

Genome-Wide Association Study for Plant Height and Grain Yield in Rice under Contrasting Moisture Regimes

Non‐redundant functions of the dimeric ABA receptor BdPYL1 in the grass Brachypodium

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