Characterization and functional analysis of a plastidial FAD6 gene and its promoter in the mesocarp of oil palm (Elaeis guineensis)

Zhu, Shousong; Zhu, Zhiyong; Wang, Herong; Wang, Lei; Cheng, Li; Yang, Yuan; Zheng, Yusheng; Li, Dongdong

doi:10.1016/j.scienta.2018.05.042

Cited by 12 publications

(5 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In fact, improved promoters are always in demand because there is no 'perfect' promoter for a variety of cultural situations [42]. Although several seed-specific promoters of various oil crops are identified [12,[43][44][45][46], the promoter that precisely regulates ricinoleic acid has not yet been reported. In our study, based on the promoter deletion strategies, we discovered that the GUS accumulation varied in tissues under the control of different deleted fragments of the RcFAH12 promoter.…”

Section: Rcfah12 Promoter Can Be Used For Genetic Engineeringmentioning

confidence: 99%

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Wang

et al. 2022

Separations

View full text Add to dashboard Cite

Castor (Ricinus communis L.) seed oil is the commercial source of ricinoleate, a valuable raw material used in many industries. Oleoyl-12-hydroxylase (RcFAH12) is a key enzyme in the biosynthesis of ricinoleate, accumulating nearly 90% of the triacylglycerol in castor seeds. Little is known about the transcriptional regulation of RcFAH12. We used rapid amplification of cDNA 5′ ends (5′RACE) to locate the transcription start site (TSS) of RcFAH12, and the sequence of a 2605 bp region, −2506~+99, surrounding the TSS was cloned. We then investigated these regions to promote β-glucuronidase (GUS) expression in transgenic Arabidopsis by the progressive 5′ and 3′ deletions strategies. The GUS staining showed that the GUS accumulation varied in tissues under the control of different deleted fragments of RcFAH12. In addition, the GUS expression driven by the RcFAH12 promoter markedly accumulated in transgenic seeds, which indicated that RcFAH12 might play an important role in the biosynthesis of ricinoleic acid. This study will lay a potential foundation for developing a tissue-specific promoter in oil-seed crops.

show abstract

Section: Rcfah12 Promoter Can Be Used For Genetic Engineeringmentioning

confidence: 99%

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Wang

et al. 2022

Separations

View full text Add to dashboard Cite

show abstract

“…Fatty acids are the major constituent of plant cell membrane. Fatty acid desaturase (FAD) is a critical enzyme in plant lipid metabolism, the FAD gene family mediates the ratio of saturated to unsaturated fatty acid resulting in affecting the plasma membrane fluidity and stability under stress ( Zhu et al, 2018 ). Thus, the FAD gene family plays an essential role in stress response besides plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification and characterization of FAD family genes in barley

Cao,

Du,

et al. 2024

PeerJ

View full text Add to dashboard Cite

Fatty acid desaturases (FADs) play pivotal roles in determining plant stress tolerance. Barley is the most salt-tolerant cereal crop. In this study, we performed genome-wide identification and characterization analysis of the FAD gene family in barley (Hordeum vulgare). A total of 24 HvFADs were identified and divided into four subfamilies based on their amino acid sequence similarity. HvFADs unevenly distributed on six of seven barley chromosomes, and three clusters of HvFADs mainly occurred on the chromosome 2, 3 and 6. Segmental duplication events were found to be a main cause for the HvFAD gene family expansion. The same HvFAD subfamily showed the relatively consistent exon-intron composition and conserved motifs of HvFADs. Cis-element analysis in HvFAD promoters indicated that the expression of HvFADs may be subject to complex regulation, especially stress-responsive elements that may involve in saline-alkaline stress response. Combined transcriptomic data with quantitative experiments, at least five HvFADs highly expressed in roots under salt or alkali treatment, suggesting they may participate in saline or alkaline tolerance in barley. This study provides novel and valuable insights for underlying salt/alkali-tolerant mechanisms in barley.

show abstract

“…FAs include saturated, monounsaturated, and polyunsaturated fatty acids. Fatty acid desaturases (FADs) are key enzymes in the biosynthesis of lipid metabolism, and they can insert a double bond at a specific location during their involvement in fatty acid metabolism, which is also a process that converts saturated fatty acids into unsaturated fatty acids [1]. Based on their solubility, FADs can be classified into soluble desaturases and membrane-bound desaturases [2].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Identification and Analysis of Fatty Acid Desaturase Gene Expression at Different Temperatures in Tausonia pullulans 6A7

Gong,

Cong,

Liu

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Tausonia pullulans 6A7 is a low-temperature yeast strain that can produce lipases. Yeast, which is made up of chassis cells, is an important part of synthetic biology, and the use of the lipase-producing properties of T. pullulans 6A7 for the production of fatty acids provides a new pathway for targeted synthesis in yeast cell factories. In this study, we performed RNA-seq on lipase-producing T. pullulans 6A7 at different temperatures (15 °C, 20 °C, 20 °C without corn oil, and 25 °C). Therefore, a total of 8455 differentially expressed genes were screened, and 16 of them were FAD candidate genes. A Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of group A (15 °C) vs. group D (25 °C) showed that the pathways of fatty acid biosynthesis (map00061) and the biosynthesis of unsaturated fatty acids (map01040) were significantly enriched. In the proposed temporal analysis of differentially expressed genes among the four temperature modulations, we found differentially expressed genes in nine clusters that had the same expression trends; these genes may be jointly involved in multiple biological processes in T. pullulans 6A7. In addition, we found 16 FAD candidate genes involved in fatty acid biosynthesis, and the expression of these genes had similar expression in the transcriptome trends with the different temperature treatments. These findings will help in future in-depth studies of the function and molecular mechanisms of these important FAD genes involved in fatty acid metabolism in yeast, and they could also be conducive to the establishment of a cellular factory for targeted fatty acid production by using yeast.

show abstract

Characterization and functional analysis of a plastidial FAD6 gene and its promoter in the mesocarp of oil palm (Elaeis guineensis)

Cited by 12 publications

References 28 publications

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Genome-wide identification and characterization of FAD family genes in barley

Transcriptome Identification and Analysis of Fatty Acid Desaturase Gene Expression at Different Temperatures in Tausonia pullulans 6A7

Contact Info

Product

Resources

About