Characterization and functional activity of human Rh monoclonal antibodies

Kumpel, Belinda M.; Jackson, Dave

doi:10.1016/s1246-7820(96)80061-9

Cited by 6 publications

(2 citation statements)

References 5 publications

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“…At 14 out of the 15 positions corresponding to these residues, the macaque The low reactivity of RF with the human IgG 1 anti-D MoAb we used here is certainly not explained by an insufficient coating of RBCs. First, the anti-D was used at saturating concentrations and the density of its antigenic target (the human RhD antigen) was identical (40 000 sites per RBC [37]) to that of the target of the macaque monoclonal Cyn2-4D5, which is specific for the Rh17 epitope of the RhCcEe-related polypeptide [38]. Second, the density of the coating obtained with the human IgG 1 anti-D was twice that obtained with the macaque Cyn2-4D5 MoAb, as confirmed by flow cytometry (see Table 1).…”

Section: Discussionmentioning

confidence: 99%

Antibodies against Macaque Monoclonal Immunoglobulin G in Rheumatoid Arthritis

Fortenfant¹,

Garrette-Gaston²,

Oksman³

et al. 1999

Scand J Immunol

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The presence of rheumatoid factors (RF) in the serum of rheumatoid arthritis (RA) patients is commonly evidenced by agglutination tests: the Waaler-Rose assay, based on the use of human red blood cells (RBCs) coated with rabbit anti-RBC antibodies, and the latex test, which uses latex particles coated with denatured human immunoglobulin G (IgG). The aim of the present study was to characterize the RF able to agglutinate human RBCs coated with macaque antihuman RBC IgG antibodies secreted from macaque-mouse heterohybridomas (two from rhesus monkey and one from crab-eating macaque). Human RBCs coated with macaque monoclonal antibodies (MacMoAbs) were used for agglutination tests and these were carried out in parallel with standard tests (Waaler-Rose and latex agglutination tests) on sera from 82 RA patients, 86 patients with other forms of inflammatory chronic arthritis and 47 healthy human subjects. MacMoAb-coated RBCs identified RF in the sera of 66% patients with RA. By contrast, the frequency of positive sera in other inflammatory diseases was 5% and all 47 healthy controls were negative. Antimacaque IgG antibodies were found to be more specific for RF than standard tests, in the sera of patients with RA.

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Section: Discussionmentioning

confidence: 99%

Antibodies against Macaque Monoclonal Immunoglobulin G in Rheumatoid Arthritis

Fortenfant¹,

Garrette-Gaston²,

Oksman³

et al. 1999

Scand J Immunol

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“…IgG monoclonal anti-D antibodies, with known serological specificity, have been evaluated in various in vitro systems to test how effective the mAbs are at interacting with immune system effector cells. The variation of monoclonal anti-D in these functional tests has also been studied in the international monoclonal workshops [19,27,28]. Each assay tests efficacy at binding to different Fc receptors.…”

Section: Functional Activitymentioning

confidence: 99%

Monoclonal anti‐D for immunoprophylaxis

Scott¹

2001

Vox Sanguinis

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Routine antenatal prophylaxis with anti-D has become accepted as desirable, but concerns have been expressed about the adequacies of supply and safety of polyclonal anti-D. Human monoclonal anti-D has been produced using Epstein-Barr virus (EBV)-transformed peripheral B cells, sometimes coupled with fusions to myeloma cell lines. More recently, molecular biology techniques have been used to produce human monoclonal anti-D in a variety of different ways. Many monoclonal antibodies (mAbs) have been characterized for fine specificity and in vitro functional activity in International Workshops. Two mAbs have been shown to cause red cell clearance and immunosuppression in male volunteers. Considerations for the future development of monoclonal anti-D for prophylactic use are reviewed.

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On the mechanism of tolerance to the Rh D antigen mediated by passive anti-D (Rh D prophylaxis)

Kumpel

2002

Immunology Letters

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Characterization and functional activity of human Rh monoclonal antibodies

Cited by 6 publications

References 5 publications

Antibodies against Macaque Monoclonal Immunoglobulin G in Rheumatoid Arthritis

Antibodies against Macaque Monoclonal Immunoglobulin G in Rheumatoid Arthritis

Monoclonal anti‐D for immunoprophylaxis

On the mechanism of tolerance to the Rh D antigen mediated by passive anti-D (Rh D prophylaxis)

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