Characterization and fine mapping of a lesion mimic mutant (Lm5) with enhanced stripe rust and powdery mildew resistance in bread wheat (Triticum aestivum L.)

Liu, Cong; Liu, Huan; Jian, Wang; Pan, Qi; Wang, Yue; Wu, Kunyan; Jia, Peiying; Mu, Yang; Tang, Huaping; Xu, Qiang; Jiang, Qiantao; Liu, Yaxi; Qi, Pengfei; Zhang, Xiaojun; Huang, Lin; Chen, Guoyue; Wang, Jirui; Wei, Yuming; Zheng, Youliang; Gou, Lulu; Yao, Qifu; Lan, Xiujin; Ma, Jian

doi:10.1007/s00122-021-03973-1

Cited by 9 publications

(12 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The homozygous and stable wheat lesion mimic mutant (MC21) was isolated from an EMS-induced Chuannong 16 (CN16) mutant bank [ 42 ]. A pair of NILs (NIL- Lm5 W and NIL- Lm5 M ) was constructed from the BC 1 F 4 population crossed by MC21 and CN16 in the 2020–2021 growing season (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The plants were individually sowed and harvested. Specifically, each line was planted in 1.5 m single rows with 0.3 m spacing between rows, with a sowing density of 15 seeds per row, i.e., plants within a row were spaced 0.1 m apart [ 42 ]. All field trials were irrigated and managed following the local standard practices [ 42 , 43 ].…”

Section: Methodsmentioning

confidence: 99%

“…Only a few lesion mimic genes have been identified and mapped in wheat, for instance, lm [ 38 ], lm1 [ 39 ], lm2 [ 39 ], lm3 [ 40 ], and Lm4 [ 41 ]. Recently, Lm5 was fine-mapped to a narrow region on the chromosome arm 2AL [ 42 ]. In this study, a pair of near-isogenic lines (NILs) with and without lesion spots formation was used in transcriptomic and metabolomics analysis to identify differentially expressed genes (DEGs), differentially accumulated metabolites (DAMs), and key pathways.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integration of transcriptomics, metabolomics, and hormone analysis revealed the formation of lesion spots inhibited by GA and CTK was related to cell death and disease resistance in bread wheat (Triticum aestivum L.)

Li,

Yan,

Liu

et al. 2024

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

Background Wheat is one of the important grain crops in the world. The formation of lesion spots related to cell death is involved in disease resistance, whereas the regulatory pathway of lesion spot production and resistance mechanism to pathogens in wheat is largely unknown. Results In this study, a pair of NILs (NIL-Lm5W and NIL-Lm5M) was constructed from the BC1F4 population by the wheat lesion mimic mutant MC21 and its wild genotype Chuannong 16. The formation of lesion spots in NIL-Lm5M significantly increased its resistance to stripe rust, and NIL-Lm5M showed superiour agronomic traits than NIL-Lm5W under stripe rust infection.Whereafter, the NILs were subjected to transcriptomic (stage N: no spots; stage S, only a few spots; and stage M, numerous spots), metabolomic (stage N and S), and hormone analysis (stage S), with samples taken from normal plants in the field. Transcriptomic analysis showed that the differentially expressed genes were enriched in plant-pathogen interaction, and defense-related genes were significantly upregulated following the formation of lesion spots. Metabolomic analysis showed that the differentially accumulated metabolites were enriched in energy metabolism, including amino acid metabolism, carbohydrate metabolism, and lipid metabolism. Correlation network diagrams of transcriptomic and metabolomic showed that they were both enriched in energy metabolism. Additionally, the contents of gibberellin A7, cis-Zeatin, and abscisic acid were decreased in leaves upon lesion spot formation, whereas the lesion spots in NIL-Lm5M leaves were restrained by spaying GA and cytokinin (CTK, trans-zeatin) in the field. Conclusion The formation of lesion spots can result in cell death and enhance strip rust resistance by protein degradation pathway and defense-related genes overexpression in wheat. Besides, the formation of lesion spots was significantly affected by GA and CTK. Altogether, these results may contribute to the understanding of lesion spot formation in wheat and laid a foundation for regulating the resistance mechanism to stripe rust.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Integration of transcriptomics, metabolomics, and hormone analysis revealed the formation of lesion spots inhibited by GA and CTK was related to cell death and disease resistance in bread wheat (Triticum aestivum L.)

Li,

Yan,

Liu

et al. 2024

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiment was conducted to detect the expression levels of key genes involved in the wheat starch synthesis pathway, including GBSSI , GBSSII , SBEI, SBEII, SSI, SSII, SSIIIa, SSIV, TaAGPL1, TaAGPS1a, TaBEI, TaBEIIa, TaBEIIb , and TaBEIII . The experimental method for qRT-PCR was referenced from previous reports ( Li et al., 2022 ). Actin was selected as the reference gene ( Wang et al., 2014 ), and the relative quantification formula (2 −ΔΔ Ct) ± standard error of the mean (SEM) was used to evaluate quantitative variation further.…”

Section: Methodsmentioning

confidence: 99%

Identification of candidate gene for the defective kernel phenotype using bulked segregant RNA and exome capture sequencing methods in wheat

et al. 2023

Self Cite

View full text Add to dashboard Cite

Wheat is a significant source of protein and starch worldwide. The defective kernel (Dek) mutant AK-3537, displaying a large hollow area in the endosperm and shrunken grain, was obtained through ethyl methane sulfonate (EMS) treatment of the wheat cultivar Aikang 58 (AK58). The mode of inheritance of the AK-3537 grain Dek phenotype was determined to be recessive with a specific statistical significance level. We used bulked segregant RNA-seq (BSR-seq), BSA-based exome capture sequencing (BSE-seq), and the ΔSNP-index algorithm to identify candidate regions for the grain Dek phenotype. Two major candidate regions, DCR1 (Dek candidate region 1) and DCR2, were identified on chromosome 7A between 279.98 and 287.93 Mb and 565.34 and 568.59 Mb, respectively. Based on transcriptome analysis and previous reports, we designed KASP genotyping assays based on SNP variations in the candidate regions and speculated that the candidate gene is TraesCS7A03G0625900 (HMGS-7A), which encodes a 3-hydroxy-3-methylglutaryl-CoA synthase. One SNP variation located at position 1,049 in the coding sequence (G>A) causes an amino acid change from Gly to Asp. The research suggests that functional changes in HMGS-7A may affect the expression of key enzyme genes involved in wheat starch syntheses, such as GBSSII and SSIIIa.

show abstract

“…tritici ) resistance in wheat ( Triticum aestivum L.) (Wang et al., 2016), Lm5 enhances resistance to powdery mildew and stripe rust ( Puccinia striiformis f.sp. tritici ) in wheat (Li et al., 2021), and Rp1‐D21 confers resistance to rust ( Puccinia sorghi ) in maize (Smith et al., 2010). In wheat, the complementary genes Necrosis 1 ( Ne1 ) and Necrosis 2 ( Ne2 ) cause hybrid necrosis (Chu et al., 2006; Tsunewaki, 1970), a type of hybrid postzygotic incompatibility associated with necrosis on the leaves of F 1 seedlings, stunted growth and sometimes lethality caused by deleterious epistatic interaction with a range of symptoms connected to multiple alleles at both loci.…”

Section: Introductionmentioning

confidence: 99%

Cloning the barley nec3 disease lesion mimic mutant using complementation by sequencing

et al. 2022

View full text Add to dashboard Cite

Disease lesion mimic (DLM) or necrotic mutants display necrotic lesions in the absence of pathogen infections. They can show improved resistance to some pathogens and their molecular dissection can contribute to revealing components of plant defense pathways. Although forward-genetics strategies to find genes causal to mutant phenotypes are available in crops, these strategies require the production of experimental cross populations, mutagenesis, or gene editing and are time-and resource-consuming or may have to deal with regulated plant materials. In this study, we described a collection of 34 DLM mutants in barley (Hordeum vulgare L.) and applied a novel method called complementation by sequencing (CBS), which enables the identification of the gene responsible for a mutant phenotype given the availability of two or more chemically mutagenized individuals showing the same phenotype.Complementation by sequencing relies on the feasibility to obtain all induced mutations present in chemical mutants and on the low probability that different individuals share the same mutated genes. By CBS, we identified a cytochrome P450 CYP71P1 gene as responsible for orange blotch DLM mutants, including the historical barley nec3 locus. By comparative phylogenetic analysis we showed that CYP71P1 gene family emerged early in angiosperm evolution but has been recurrently lost in some lineages including Arabidopsis thaliana (L.) Heynh. Complementation by sequencing is a straightforward cost-effective approach to clone genes controlling phenotypes in a chemically mutagenized collection. The TILLMore (TM) collection will be instrumental for understanding the molecular basis of DLM phenotypes and to contribute knowledge about mechanisms of host-pathogen interaction.

show abstract

Characterization and fine mapping of a lesion mimic mutant (Lm5) with enhanced stripe rust and powdery mildew resistance in bread wheat (Triticum aestivum L.)

Cited by 9 publications

References 73 publications

Integration of transcriptomics, metabolomics, and hormone analysis revealed the formation of lesion spots inhibited by GA and CTK was related to cell death and disease resistance in bread wheat (Triticum aestivum L.)

Integration of transcriptomics, metabolomics, and hormone analysis revealed the formation of lesion spots inhibited by GA and CTK was related to cell death and disease resistance in bread wheat (Triticum aestivum L.)

Identification of candidate gene for the defective kernel phenotype using bulked segregant RNA and exome capture sequencing methods in wheat

Cloning the barley nec3 disease lesion mimic mutant using complementation by sequencing

Contact Info

Product

Resources

About