Characterization and Fine Mapping of a Necrotic Leaf Mutant in Maize (Zea mays L.)

Wang, Lijing; Han, Shuai; Zhong, Shiyi; Wei, Haizhong; Zhang, Yanjun; Zhao, Yan; Liu, Baoshen

doi:10.1016/j.jgg.2013.04.004

Cited by 14 publications

(15 citation statements)

References 44 publications

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“…The initiation of necrosis at the third-leaf stage, the spread of necrosis from the leaf tip to the entire leaf, and early necrotic lethality were similar to those of lethal necrotic mutants in maize nec4, nec5, nec7, and olive-necrotic-8147 [21] Figure 3 and Supplementary Table S3). These phenotypes have also been reported in a necrotic mutant of maize, nec-t [22]. nec-t has abnormal chloroplast development and lacks photosynthetic ability.…”

Section: Discussionsupporting

confidence: 52%

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Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (<i>Oryza sativa</i> L.)

Mbaraka¹,

Yamagata²,

Yoshimura³

et al. 2017

AJPS

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The spread of tissue necrosis leads to plant death. We isolated 18 lethal necrotic mutants induced in rice (Oryza sativa L.) by gamma-irradiation. The necrotic lethality among the 18 mutants was controlled by single recessive genes designated necrotic lethality1 (nec1) to nec18. These mutants display pale-green leaves from the third-leaf stage and leaf-tip necrosis, which spreads to the whole plant, killing it. Genetic mapping and histochemical analysis of the lethal necrotic mutants were conducted. At least four independent loci on chromosomes 2 and 4 controlled necrotic lethality. Therefore, the genetic causes of lethal necrosis vary among mutant stocks. Histochemical analysis at 12 days after sowing showed that H 2 O 2 accumulated in the necrotic parts of leaves, and that cell death occurred throughout the leaf. Mutants for early necrotic lethality (<24 days to lethality) were characterized by the rapid spread of H 2 O 2 accumulation throughout the third leaf. Mutants for late necrotic lethality (>35 days to lethality) were characterized by the incomplete spread of H 2 O 2 accumulation within the third leaf.

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Section: Discussionsupporting

confidence: 52%

“…at least eight recessive mutants (nec1 -nec7, nec-t; [21] [22] showed leaf discoloration, necrotic leaf tips, spots, or transverse bands before death. However, rice mutants showing necrotic lethality have not yet been isolated.…”

Section: Introductionmentioning

confidence: 99%

Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (<i>Oryza sativa</i> L.)

Mbaraka¹,

Yamagata²,

Yoshimura³

et al. 2017

AJPS

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“…A potential candidate gene underlying the detected QTL may be Nec*-t . Nec*-t is responsible for stress induced reduction of chloroplasts and chlorophyll [ 55 ] potentially explaining the observed effects of this chromosomal segment on vigor, senescence, EPP and grain yield.…”

Section: Discussionmentioning

confidence: 99%

Identification of QTL for Early Vigor and Stay-Green Conferring Tolerance to Drought in Two Connected Advanced Backcross Populations in Tropical Maize (Zea mays L.)

et al. 2016

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We aimed to identify quantitative trait loci (QTL) for secondary traits related to grain yield (GY) in two BC1F2:3 backcross populations (LPSpop and DTPpop) under well-watered (4 environments; WW) and drought stressed (6; DS) conditions to facilitate breeding efforts towards drought tolerant maize. GY reached 5.6 and 5.8 t/ha under WW in the LPSpop and the DTPpop, respectively. Under DS, grain yield was reduced by 65% (LPSpop) to 59% (DTPpop) relative to WW. GY was strongly associated with the normalized vegetative index (NDVI; r ranging from 0.61 to 0.96) across environmental conditions and with an early flowering under drought stressed conditions (r ranging from -0.18 to -0.25) indicative of the importance of early vigor and drought escape for GY. Out of the 105 detected QTL, 53 were overdominant indicative of strong heterosis. For 14 out of 18 detected vigor QTL, as well as for eight flowering time QTL the trait increasing allele was derived from CML491. Collocations of early vigor QTL with QTL for stay green (bin 2.02, WW, LPSpop; 2.07, DS, DTPpop), the number of ears per plant (bins 2.02, 2.05, WW, LPSpop; 5.02, DS, LPSpop) and GY (bin 2.07, WW, DTPpop; 5.04, WW, LPSpop), reinforce the importance of the observed correlations. LOD scores for early vigor QTL in these bins ranged from 2.2 to 11.25 explaining 4.6 (additivity: +0.28) to 19.9% (additivity: +0.49) of the observed phenotypic variance. A strong flowering QTL was detected in bin 2.06 across populations and environmental conditions explaining 26–31.3% of the observed phenotypic variation (LOD: 13–17; additivity: 0.1–0.6d). Improving drought tolerance while at the same time maintaining yield potential could be achieved by combining alleles conferring early vigor from the recurrent parent with alleles advancing flowering from the donor. Additionally bin 8.06 (DTPpop) harbored a QTL for GY under WW (additivity: 0.27 t/ha) and DS (additivity: 0.58 t/ha). R2 ranged from 0 (DTPpop, WW) to 26.54% (LPSpop, DS) for NDVI, 18.6 (LPSpop, WW) to 42.45% (LPSpop, DS) for anthesis and from 0 (DTPpop, DS) to 24.83% (LPSpop, WW) for GY. Lines out-yielding the best check by 32.5% (DTPpop, WW) to 60% (DTPpop, DS) for all population-by-irrigation treatment combination (except LPSpop, WW) identified are immediately available for the use by breeders.

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“…The Alexander′s staining solution 34 was used to test the viability of the pollen grains in the permissive conditions. For TEM 35 , anthers were dissected and fixed in fresh 0.1 M PIPES buffer (pH 6.8). A 2% osmium tetroxide stain was applied with brief washes, followed by dehydration in an acetone gradient.…”

Section: Methodsmentioning

confidence: 99%

Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize

et al. 2020

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Small RNAs play important roles during plant development by regulating transcript levels of target mRNAs, maintaining genome integrity, and reinforcing DNA methylation. Dicer-like 5 (Dcl5) is proposed to be responsible for precise slicing in many monocots to generate diverse 24-nt phased, secondary small interfering RNAs (phasiRNAs), which are exceptionally abundant in meiotic anthers of diverse flowering plants. The importance and functions of these phasiRNAs remain unclear. Here, we characterized several mutants of dcl5, including alleles generated by the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system and a transposon-disrupted allele. We report that dcl5 mutants have few or no 24-nt phasiRNAs, develop short anthers with defective tapetal cells, and exhibit temperaturesensitive male fertility. We propose that DCL5 and 24-nt phasiRNAs are critical for fertility under growth regimes for optimal yield.

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Characterization and Fine Mapping of a Necrotic Leaf Mutant in Maize (Zea mays L.)

Cited by 14 publications

References 44 publications

Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (<i>Oryza sativa</i> L.)

Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (<i>Oryza sativa</i> L.)

Identification of QTL for Early Vigor and Stay-Green Conferring Tolerance to Drought in Two Connected Advanced Backcross Populations in Tropical Maize (Zea mays L.)

Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize

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Characterization and Fine Mapping of a Necrotic Leaf Mutant in Maize (Zea mays L.)

Cited by 14 publications

References 44 publications

Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (&lt;i&gt;Oryza sativa&lt;/i&gt; L.)

Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (&lt;i&gt;Oryza sativa&lt;/i&gt; L.)

Identification of QTL for Early Vigor and Stay-Green Conferring Tolerance to Drought in Two Connected Advanced Backcross Populations in Tropical Maize (Zea mays L.)

Dicer-like 5 deficiency confers temperature-sensitive male sterility in maize

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Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (<i>Oryza sativa</i> L.)

Genetic Mapping and Characterization of Lethal Necrotic Mutants in Rice (<i>Oryza sativa</i> L.)