Characterization and expression of SpHsp60 in hemocytes after challenge to bacterial, osmotic and thermal stress from the mud crab Scylla paramamosain

Yang, Yanan; Ye, Haihui; Huang, Huiyang; Li, Shaojing; Zeng, Xianglan; Gong, Jie; Huang, Xiaoshuai

doi:10.1016/j.fsi.2013.07.029

Cited by 31 publications

(16 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Mitochondrial HSP60 is involved in maintaining the mitochondrial function and DNA stability42 and can protect mitochondria against temperature stress424344 as well as other proteotoxic insults such as exposure to toxic metals234546. Inability of LN clams to overexpress HSP60 during acute heat stress may contribute to the damage of their mitochondria and lead to a decrease in the aerobic capacity at elevated temperatures.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Acclimation to Different Thermal Regimes Affects Molecular Responses to Heat Stress in a Freshwater Clam Corbicula Fluminea

Falfushynska

Phan

Sokolova

2016

Sci Rep

View full text Add to dashboard Cite

Global climate change (GCC) can negatively affect freshwater ecosystems. However, the degree to which freshwater populations can acclimate to long-term warming and the underlying molecular mechanisms are not yet fully understood. We used the cooling water discharge (CWD) area of a power plant as a model for long-term warming. Survival and molecular stress responses (expression of molecular chaperones, antioxidants, bioenergetic and protein synthesis biomarkers) to experimental warming (20–41 °C, +1.5 °C per day) were assessed in invasive clams Corbicula fluminea from two pristine populations and a CWD population. CWD clams had considerably higher (by ~8–12 °C) lethal temperature thresholds than clams from the pristine areas. High thermal tolerance of CWD clams was associated with overexpression of heat shock proteins HSP70, HSP90 and HSP60 and activation of protein synthesis at 38 °C. Heat shock response was prioritized over the oxidative stress response resulting in accumulation of oxidative lesions and ubiquitinated proteins during heat stress in CWD clams. Future studies should determine whether the increase in thermal tolerance in CWD clams are due to genetic adaptation and/or phenotypic plasticity. Overall, our findings indicate that C. fluminea has potential to survive and increase its invasive range during warming such as expected during GCC.

show abstract

Section: Discussionmentioning

confidence: 99%

Long-Term Acclimation to Different Thermal Regimes Affects Molecular Responses to Heat Stress in a Freshwater Clam Corbicula Fluminea

Falfushynska

Phan

Sokolova

2016

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In order to determine whether SpEcR dsRNA treatment had a non-specific silencing effect or led to tissue lethality, the expression level of the housekeeping gene, SpGapdh, was synchronously detected (Das & Durica 2013, Yang et al 2013. It was found that the addition of SpEcR dsRNA did not have any significant effect on the expression of the SpGapdh gene in ovarian explants as compared with that of the Gfp-treated controls (Fig.…”

Section: Gene Silencing With Specr Dsrnamentioning

confidence: 99%

“…SSCT). The hybridized tissue sections were incubated with anti-DIG alkaline-phosphatase-conjugated antibody (Roche) and signals were visualized with the colorimetric substrates nitroblue tetrazolium/4-bromo-4-chloro-30-indolylphosphate (Yang et al 2013). The riboprobe template for SpEcR was generated by PCR from ovary cDNA using the specific primers TEcR F (1079-1100 bp), TEcR R (1387-1410 bp), and T7 (Table 1).…”

Section: Localization Of Specr In the Ovary By In Situ Hybridizationmentioning

confidence: 99%

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

Gong¹,

Ye²,

Xie³

et al. 2015

Journal of Endocrinology

Self Cite

View full text Add to dashboard Cite

In arthropods, it is known that ecdysteroids regulate molting, limb regeneration, and reproduction through activation of the ecdysone receptor (EcR). However, the ecdysteroid signaling pathway for promotion of ovarian development in crustaceans is still unclear. In this study, three cDNA isoforms of EcR were cloned from the mud crab Scylla paramamosain. qRT-PCR revealed that the SpEcR mRNA was abundant in the eyestalk, ovary and epidermis. During ovarian development, the SpEcR transcripts increased from stage I (undeveloped stage) and reached a peak at stage IV (late vitellogenic stage) before dropping to a lower level at stage V (mature stage). Meanwhile, levels of 20-hydroxyecdysone (20E) in the hemolymph, detected by HPLC-MS, displayed a similar pattern of increase with ovarian development. Results from in situ hybridization indicated that SpEcR mRNA was present in the follicular cells during vitellogenesis. Results from in vivo experiments revealed that 20E at 0.2 mg/g body weight significantly stimulated the expression of SpEcR and vitellogenin (SpVg) in female crabs during the early vitellogenic stage but not during the previtellogenic stage. This was confirmed by results from in vitro experiments which indicated that SpEcR and SpVg expression levels were significantly upregulated in early vitellogenic ovarian explants incubated with 5.0 mM 20E at 3 and 6 h but not in previtellogenic ovarian explants. Finally, results from in vitro gene silencing experiments indicated that the expression of SpEcR and SpVg in the ovary was significantly inhibited by SpEcR dsRNA. All these results together indicated that in S. paramamosain, 20E, and SpEcR, located in the follicular cells, play important roles in the promotion of ovarian development via regulating the expression of SpVg.

show abstract

“…The mud crab aquaculture industry is a valuable source of income for coastal communities in Southeast Asia (Yang et al, 2013b). However, with the rapid development of mud crab aquaculture, various diseases caused by bacteria, virus, and rickettsia-like organisms have frequently occurred and resulted in great economic losses.…”

Section: Introductionmentioning

confidence: 99%

Identification of three IFN-γ inducible lysosomal thiol reductase ( GILT )-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression

Huang

Duan

Huang

et al. 2015

Gene

View full text Add to dashboard Cite

Characterization and expression of SpHsp60 in hemocytes after challenge to bacterial, osmotic and thermal stress from the mud crab Scylla paramamosain

Cited by 31 publications

References 43 publications

Long-Term Acclimation to Different Thermal Regimes Affects Molecular Responses to Heat Stress in a Freshwater Clam Corbicula Fluminea

Long-Term Acclimation to Different Thermal Regimes Affects Molecular Responses to Heat Stress in a Freshwater Clam Corbicula Fluminea

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

Identification of three IFN-γ inducible lysosomal thiol reductase ( GILT )-like genes in mud crab Scylla paramamosain with distinct gene organizations and patterns of expression

Contact Info

Product

Resources

About