Characterization and expression of prohibitin during the mexican bean weevil (Zabrotes subfasciatus, Boheman, 1833) larvae development

Villegas-Coronado, Diana; Guzmán-Partida, Ana María; Aispuro‐Hernández, Emmanuel; Vázquez–Moreno, Luz; Huerta‐Ocampo, José Ángel; Sarabia-Sainz, Jose A.; Teran-Saavedra, Nayelli Guadalupe; Minjarez‐Osorio, Christian; Castro‐Longoria, Reina; Maldonado, Amir; Lagarda-Díaz, Irlanda

doi:10.1016/j.cbpb.2022.110770

Cited by 1 publication

(2 citation statements)

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“…[16] Furthermore, the expression of proteins recognized by PF2 varies according to the life cycle stage of the insect. [17] Interestingly, the sialylation levels and profiles of siaoglycoconjugates on the surface of and within THP-1 monocytes vary during differentiation into macrophages and subsequent polarization. [18] Considering that acute myeloid leukemia is characterized by alterations in the monocyte lineage and development, this study aims to explore the antitumor potential of PF2 by evaluating its capacity to recognize human acute leukemia cell and exert an antiproliferative effect and to advance the understanding of its mechanism of action using the monocytic THP-1 cell lines as a model in vitro.…”

Section: Introductionmentioning

confidence: 99%

“…The PF2 lectin, isolated from the seeds of the desert wild legume Olneya tesota (desert ironwood), is toxic to the Zabrotes subfasciatus beetle and displays recognition specificity for triantennary tetrasialylated glycans [16] . Furthermore, the expression of proteins recognized by PF2 varies according to the life cycle stage of the insect [17] . Interestingly, the sialylation levels and profiles of siaoglycoconjugates on the surface of and within THP‐1 monocytes vary during differentiation into macrophages and subsequent polarization [18] …”

Section: Introductionmentioning

confidence: 99%

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Antiproliferative Potential of Olneya tesota PF2 Lectin in Human Acute Monocytic Leukemia Cells

Villegas-Coronado

Soto-Guzmán

Martínez-Soto

et al. 2023

Chemistry & Biodiversity

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Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity.

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Section: Introductionmentioning

confidence: 99%