Characterization and expression of <i>MUSTN1</i> gene from different duck breeds

Zhi-xiu, Wang; Liang, Wenshuang; Li, Xiangxiang; Zhang, Jiatong; Xu, Qi; Chen, Guohong; Zhang, Hao; Chang, Guobin

doi:10.1080/10495398.2020.1828905

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Mustn1 is expressed in all musculoskeletal cell types that form bone, cartilage, tendon, and muscle (Lombardo et al, 2004). The exact function of Mustn1 remains unknown, however, its expression during skeletal muscle development in different species has been widely studied (Kim et al, 2009; Zheng et al, 2009; Liu et al, 2010; Xu et al, 2012; Gersch et al, 2012; Li et al, 2013; Xu et al, 2015; Kong et al, 2017; Camarata et al, 2016; Danzmann et al, 2016; Hadjiargyrou, 2018; Zhu et al, 2021; Wang et al, 2022). As a pan‐musculoskeletal cell marker, Mustn1 is actively expressed in areas of early cartilage, bone, and muscle formation, specifically in limb buds, brachial arches, vertebrae, and somites during embryogenesis (Lombardo et al, 2004; Liu et al, 2010; Gersch et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Mustn1 ablation in skeletal muscle results in increased glucose tolerance concomitant with upregulated GLUT expression in male mice

Kim,

Singh,

Lee

et al. 2023

Physiological Reports

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Section: Introductionmentioning

confidence: 99%

Mustn1 ablation in skeletal muscle results in increased glucose tolerance concomitant with upregulated GLUT expression in male mice

Kim,

Singh,

Lee

et al. 2023

Physiological Reports

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“…The expression of the MUSTN1 gene significantly increased from E21 to E27 ( P < 0.05), and the elevation persisted until postnatal day 7. The MUSTN1 gene plays a role in regulating skeletal muscle hypertrophy and myofusion, and it has been linked to the rapid development of the pectoral muscle in ducks [ 62 – 64 ]. The heightened expression of the MUSTN1 gene during the late embryonic phase suggests its crucial involvement in myofusion before hatching (D0) and the positive regulation of muscle growth during postnatal stages.…”

Section: Discussionmentioning

confidence: 99%

Integrating miRNA and full-length transcriptome profiling to elucidate the mechanism of muscle growth in Muscovy ducks reveals key roles for miR-301a-3p/ANKRD1

Huang,

Xiong,

Zhang

et al. 2024

BMC Genomics

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Background The popularity of Muscovy ducks is attributed not only to their conformation traits but also to their slightly higher content of breast and leg meat, as well as their stronger-tasting meat compared to that of typical domestic ducks. However, there is a lack of comprehensive systematic research on the development of breast muscle in Muscovy ducks. In addition, since the number of skeletal muscle myofibers is established during the embryonic period, this study conducted a full-length transcriptome sequencing and microRNA sequencing of the breast muscle. Muscovy ducks at four developmental stages, namely Embryonic Day 21 (E21), Embryonic Day 27 (E27), Hatching Day (D0), and Post-hatching Day 7 (D7), were used to isolate total RNA for analysis. Results A total of 68,161 genes and 472 mature microRNAs were identified. In order to uncover deeper insights into the regulation of mRNA by miRNAs, we conducted an integration of the differentially expressed miRNAs (known as DEMs) with the differentially expressed genes (referred to as DEGs) across various developmental stages. This integration allowed us to make predictions regarding the interactions between miRNAs and mRNA. Through this analysis, we identified a total of 274 DEGs that may serve as potential targets for the 68 DEMs. In the predicted miRNA‒mRNA interaction networks, let-7b, miR-133a-3p, miR-301a-3p, and miR-338-3p were the hub miRNAs. In addition, multiple DEMs also showed predicted target relationships with the DEGs associated with skeletal system development. These identified DEGs and DEMs as well as their predicted interaction networks involved in the regulation of energy homeostasis and muscle development were most likely to play critical roles in facilitating the embryo-to-hatchling transition. A candidate miRNA, miR-301a-3p, exhibited increased expression during the differentiation of satellite cells and was downregulated in the breast muscle tissues of Muscovy ducks at E21 compared to E27. A dual-luciferase reporter assay suggested that the ANKRD1 gene, which encodes a transcription factor, is a direct target of miR-301a-3p. Conclusions miR-301a-3p suppressed the posttranscriptional activity of ANKRD1, which is an activator of satellite cell proliferation, as determined with gain- and loss-of-function experiments. miR-301a-3p functions as an inducer of myogenesis by targeting the ANKRD1 gene in Muscovy ducks. These results provide novel insights into the early developmental process of black Muscovy breast muscles and will improve understanding of the underlying molecular mechanisms.

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“…Additional studies from our laboratory have illustrated that Mustn1 is primarily and selectively expressed within the musculoskeletal system [13,[16][17][18]. Also, an array of comprehensive studies across various species, such as zebrafish [19,20], trout [21], ducks [22][23][24], chickens [25][26][27][28], pigs [29], donkeys [30], minks [31], and humans [32,33], has demonstrated Mustn1 expression in various skeletal muscles, further implicating a role in this tissue. Mustn1 was also showed to be involved in the myogenic differentiation of C2C12 cells, commonly used as a model system for muscle development [17].…”

Section: Mustn1mentioning

confidence: 91%

Mustn1 in Skeletal Muscle: A Novel Regulator?

Kim,

Hadjiargyrou

2024

Genes

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Skeletal muscle is a complex organ essential for locomotion, posture, and metabolic health. This review explores our current knowledge of Mustn1, particularly in the development and function of skeletal muscle. Mustn1 expression originates from Pax7-positive satellite cells in skeletal muscle, peaks during around the third postnatal month, and is crucial for muscle fiber differentiation, fusion, growth, and regeneration. Clinically, Mustn1 expression is potentially linked to muscle-wasting conditions such as muscular dystrophies. Studies have illustrated that Mustn1 responds dynamically to injury and exercise. Notably, ablation of Mustn1 in skeletal muscle affects a broad spectrum of physiological aspects, including glucose metabolism, grip strength, gait, peak contractile strength, and myofiber composition. This review summarizes our current knowledge of Mustn1’s role in skeletal muscle and proposes future research directions, with a goal of elucidating the molecular function of this regulatory gene.

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Characterization and expression of MUSTN1 gene from different duck breeds

Cited by 5 publications

References 31 publications

Mustn1 ablation in skeletal muscle results in increased glucose tolerance concomitant with upregulated GLUT expression in male mice

Mustn1 ablation in skeletal muscle results in increased glucose tolerance concomitant with upregulated GLUT expression in male mice

Integrating miRNA and full-length transcriptome profiling to elucidate the mechanism of muscle growth in Muscovy ducks reveals key roles for miR-301a-3p/ANKRD1

Mustn1 in Skeletal Muscle: A Novel Regulator?

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