Characterization and expression analysis of PbEXP genes in the epidermis of pear (Pyrus bretschneideri Rehd.)

Heng, Wei; Yang, Jinyu; Hou, Zhaoqi; Li, Fēi; Jia, Bing; Liu, Pu; Ye, Zhenfeng; Zhu, Li

doi:10.1007/s10725-017-0315-y

Cited by 10 publications

(3 citation statements)

References 33 publications

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“…Strikingly, AP2-EREBP and MYB super-families were the largest in ST118-L-Spe and ST-SS-L-inter, while the WRKY super-family was the largest in the SS30-L-Spe. Members of these identified TFs have been reported to be associated with salt stress responses [8, 17, 57, 58]. Subsequently, the 413 TFs were searched against the Stress Responsive Transcription Factor Database (STIDB) in Arabidopsis [58] for salt-responsive genes.…”

Section: Resultsmentioning

confidence: 99%

“…Transcription factors (TFs) involved in the regulation of salt-response could be activated by multiple signal transduction pathways in plants, such as the ABA-mediated signal [1, 5, 6]. Previous studies reported that the members of TF family genes were differentially expressed in response to elevated external salinity [7], including the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/EREBP) [8], basic leucine zipper (bZIP) [6, 9], NAC [10, 11], basic helix–loop–helix (bHLH) [12], MYB [13–15] and WRKY [16, 17] gene families. In turn, these TFs could amplify the signals for gene regulation and promote the protective mechanisms in plants.…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome analysis reveals K+ transporter gene contributing to salt tolerance in eggplant

Gao

Zhou

et al. 2019

BMC Plant Biol

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BackgroundSoil salinization is one of the most crucial abiotic stresses that limit the growth and production of eggplant. The existing researches in eggplant were mostly focused on salt-induced morphological, biochemical and physiological changes, with only limited works centered on salt-response genes in eggplant at the transcriptomic level.ResultsOur preliminary work found that Zhusiqie (No.118) is salt-tolerant and Hongqie (No.30) is salt-sensitive. Consequently, they were re-named as ST118 and SS30, respectively. ST118 showed less damaged on growth and higher K+/Na+ ratios in leaves than SS30. Comparative-transcriptome analysis was used as a powerful approach to understand the salt-response mechanisms in the leaves and roots of SS30 and ST118. And it revealed that genotype-specific and organ-specific manners exist in eggplant in response to salt stress. Strikingly, the genotype-specific differentially expressed genes (DEGs) in ST118 were considered crucial to its higher salt-tolerance, because the expression patterns of common DEGs in the leaves/roots of the two eggplant genotypes were almost the same. Among them, some transcription factors have been reported to be in response to elevated external salinity, including the members of C2C2-CO-like, WRKY, MYB and NAC family. In addition, the AKT1, KAT1 and SOS1 were up-regulated only in the leaves of ST118. Furthermore, the complementation assays demonstrated that the salt-tolerances of both yeast and Arabidopsis akt1 mutants were enhanced by heterologous expression of SmAKT1.ConclusionThe comparative-transcriptome analysis indicated that the salt-tolerance can be increased by higher transcript level of some genotype-specific genes. This work revealed that eggplants seem to be more inclined to absorb K+ rather than to exclude Na+ under salt stress conditions because seven K+ transporters were significantly up-regulated, while only one Na+ transporter was similarly regulated. Finally, the complementation assays of SmAKT1, which is genotype-specific up-regulated in ST118, suggest that the other TFs and K+ transport genes were worthy of future investigation for their functions in salinity tolerance.Electronic supplementary materialThe online version of this article (10.1186/s12870-019-1663-8) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals K+ transporter gene contributing to salt tolerance in eggplant

Gao

Zhou

et al. 2019

BMC Plant Biol

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“…The expression of PbEXPB3b and PbEXP4a enhanced during russeting stage in Dangshansuli (Pyrus bretschneideri Rehd.) (Heng et al ., 2017). The DEPs of expansin provide more direct evidence of the loosening of the exocarp in russet pears.…”

Section: Stress Response and Water Channel And Transmembrane Transporter Activitymentioning

confidence: 99%

Network of multi-omics reveals the mechanism of russeting under rainwater in pears

Shi

Wang

Zhang

et al. 2020

Preprint

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Russeting occurs via the epidermal suberin accumulation model in the phenol-ester protection system in pears, especially under excessive rainfall. To clarify the role of rain in the russeting mechanism, we applied multi-omics (transcriptome, metabolome, and proteome) integrated analysis from three colored pear cultivars grown under two rainfall conditions, including a no rain control (C) and a rain-abundant (R) treatment. Broad alterations occurred in 29 overlapping differentially expressed genes (DEGs) involved in the response to stress and secondary metabolite biosynthesis. Three overlapping differentially expressed proteins (DEPs) and seven differentially expressed metabolites (DEMs) were identified among the three comparison groups.Cross-comparison of mRNA and protein data revealed co-expression of DEGs and DEPs. Suberin and phenylpropane were markedly enhanced, while cutin biosynthesis and fatty acid elongation were depressed in russet pears under R. In russet pears, the triacontane associated with wax composition was abruptly missing. Moreover, The PpyHHT1 (103966555) gene and encoded protein HHT1 (694406379), which acts as a 'bridge' between phenol-and ester-polymerization, had significantly upregulated expression. Collectively, we provide a comprehensive model of the molecular russeting mechanism, which provides powerful insight into russeting and shows the plasticity of plant defenses to cope with the harsh natural environment.

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Functional Diversity of Plant Growth-Promoting Rhizobacteria: Recent Progress and Future Prospects

Altaf

Khan

Ahmad

2019

Microbial Interventions in Agriculture and Environment

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Characterization and expression analysis of PbEXP genes in the epidermis of pear (Pyrus bretschneideri Rehd.)

Cited by 10 publications

References 33 publications

Comparative transcriptome analysis reveals K+ transporter gene contributing to salt tolerance in eggplant

Comparative transcriptome analysis reveals K+ transporter gene contributing to salt tolerance in eggplant

Network of multi-omics reveals the mechanism of russeting under rainwater in pears

Functional Diversity of Plant Growth-Promoting Rhizobacteria: Recent Progress and Future Prospects

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