Characterization and expression analysis of adipokinetic hormone and its receptor in eusocial aphid Pseudoregma bambucicola

Jedličková, Veronika; Jedlička, Pavel; Lee, How‐Jing

doi:10.1016/j.ygcen.2015.09.032

Cited by 17 publications

(13 citation statements)

References 49 publications

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“…The analyzed insect AKH receptors form a separate cluster and are distinct from ACP, Crz and GnRH receptors ( Figure 1 ). This phylogeny is well in line with what other studies have described [ 11 , 35 , 42 , 43 ].…”

Section: Resultssupporting

confidence: 93%

Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors

Marchal

Schellens

Monjon

et al. 2018

IJMS

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Adipokinetic hormone (AKH) is a highly researched insect neuropeptide that induces the mobilization of carbohydrates and lipids from the fat body at times of high physical activity, such as flight and locomotion. As a naturally occurring ligand, AKH has undergone quite a number of amino acid changes throughout evolution, and in some insect species multiple AKHs are present. AKH acts by binding to a rhodopsin-like G protein-coupled receptor, which is related to the vertebrate gonadotropin-releasing hormone receptors. In the current study, we have cloned AKH receptors (AKHRs) from seven different species, covering a wide phylogenetic range of insect orders: the fruit fly, Drosophila melanogaster, and the yellow fever mosquito, Aedes aegypti (Diptera); the red flour beetle, Tribolium castaneum, and the large pine weevil, Hylobius abietis (Coleoptera); the honeybee, Apis mellifera (Hymenoptera); the pea aphid, Acyrthosiphon pisum (Hemiptera); and the desert locust, Schistocerca gregaria (Orthoptera). The agonistic activity of different insect AKHs, including the respective endogenous AKHs, at these receptors was tested with a bioluminescence-based assay in Chinese hamster ovary cells. All receptors were activated by their endogenous ligand in the nanomolar range. Based on our data, we can refute the previously formulated hypothesis that a functional AKH signaling system is absent in the beneficial species, Apis mellifera. Furthermore, our data also suggest that some of the investigated AKH receptors, such as the mosquito AKHR, are more selective for the endogenous (conspecific) ligand, while others, such as the locust AKHR, are more promiscuous and can be activated by AKHs from many other insects. This information will be of high importance when further analyzing the potential use of AKHRs as targets for developing novel pest control agents.

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Section: Resultssupporting

confidence: 93%

Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors

Marchal

Schellens

Monjon

et al. 2018

IJMS

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“…S5F). While Drosophila AKH has primarily been shown to affect lipid homeostasis in fat body through the AKH receptor, AKH can also directly control metabolism in the intestine [41, 42]. Therefore, these AKH-dependent effects on intestinal lipid homeostasis may be direct, or secondary to metabolic changes in the fat body.…”

Section: Resultsmentioning

confidence: 99%

Muscle Directs Diurnal Energy Homeostasis through a Myokine-Dependent Hormone Module in Drosophila

Zhao

Karpac

2017

Current Biology

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Summary Inter-tissue communication is critical to control organismal energy homeostasis in response to temporal changes in feeding and activity or external challenges. Muscle is emerging as a key mediator of this homeostatic control through consumption of lipids, carbohydrates, and amino acids, as well as governing systemic signaling networks. However, it remains less clear how energy substrate usage tissues, such as muscle, communicate with energy substrate storage tissues in order to adapt with diurnal changes in energy supply and demand. Using Drosophila, we show here that muscle plays a crucial physiological role in promoting systemic synthesis and accumulation of lipids in fat storage tissues, which subsequently impacts diurnal changes in circulating lipid levels. Our data reveal that the metabolic transcription factor Foxo governs expression of the cytokine Unpaired 2 (Upd2) in skeletal muscle, which acts as a myokine to control glucagon-like adipokinetic hormone (AKH) secretion from specialized neuroendocrine cells. Circulating AKH levels, in turn, regulate lipid homeostasis in fat body/adipose and the intestine. Our data also reveal that this novel myokine-dependent hormone module is critical to maintain diurnal rhythms in circulating lipids. This tissue cross-talk provides a putative mechanism that allows muscle to integrate autonomous energy demand with systemic energy storage and turnover. Together, these findings reveal a diurnal inter-tissue signaling network between muscle and fat-storage tissues that constitutes an ancestral mechanism governing systemic energy homeostasis.

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“…Moreover, the expression of AKH in the brain stays high over the course of the queen's life, and is higher than in workers and males. Studies on the regulation of AKH and AKHR gene expression in different phenotypes or in response to changes in the environment are limited to aphid models so far (Jedličková et al, 2015). The possible involvement of AKH/AKHR signaling in energy storage mobilization during diapause emerged with records of high sensitivity of diapausing P. apterus females to AKH application when compared to non-diapausing conspecifics (Socha and Kodrík, 1999).…”

Section: Resultsmentioning

confidence: 99%

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

Jedlička

Ernst

Votavová³

et al. 2016

Front. Physiol.

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Understanding the social evolution leading to insect eusociality requires, among other, a detailed insight into endocrine regulatory mechanisms that have been co-opted from solitary ancestors to play new roles in the complex life histories of eusocial species. Bumblebees represent well-suited models of a relatively primitive social organization standing on the mid-way to highly advanced eusociality and their queens undergo both, a solitary and a social phase, separated by winter diapause. In the present paper, we characterize the gene expression levels of major endocrine regulatory pathways across tissues, sexes, and life-stages of the buff-tailed bumblebee, Bombus terrestris, with special emphasis on critical stages of the queen's transition from solitary to social life. We focused on fundamental genes of three pathways: (1) Forkhead box protein O and insulin/insulin-like signaling, (2) Juvenile hormone (JH) signaling, and (3) Adipokinetic hormone signaling. Virgin queens were distinguished by higher expression of forkhead box protein O and downregulated insulin-like peptides and JH signaling, indicated by low expression of methyl farnesoate epoxidase (MFE) and transcription factor Krüppel homolog 1 (Kr-h1). Diapausing queens showed the expected downregulation of JH signaling in terms of low MFE and vitellogenin (Vg) expressions, but an unexpectedly high expression of Kr-h1. By contrast, reproducing queens revealed an upregulation of MFE and Vg together with insulin signaling. Surprisingly, the insulin growth factor 1 (IGF-1) turned out to be a queen-specific hormone. Workers exhibited an expression pattern of MFE and Vg similar to that of reproducing queens. Males were characterized by high Kr-h1 expression and low Vg level. The tissue comparison unveiled an unexpected resemblance between the fat body and hypopharyngeal glands across all investigated genes, sexes, and life stages.

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Characterization and expression analysis of adipokinetic hormone and its receptor in eusocial aphid Pseudoregma bambucicola

Cited by 17 publications

References 49 publications

Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors

Analysis of Peptide Ligand Specificity of Different Insect Adipokinetic Hormone Receptors

Muscle Directs Diurnal Energy Homeostasis through a Myokine-Dependent Hormone Module in Drosophila

Gene Expression Dynamics in Major Endocrine Regulatory Pathways along the Transition from Solitary to Social Life in a Bumblebee, Bombus terrestris

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