Characterization and expression analysis of the interferon regulatory factor (IRF) gene family in zig-zag eel (Mastacembelus armatus) against Aeromonas veronii infection

Han, Chong; Huang, Wen Wen; Peng, Suhan; Zhou, Jiangwei; Zhan, Huawei; Li, Wenjun; Ju, Gong; Li, Qiang

doi:10.1016/j.dci.2022.104622

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…In addition, studies in mammals have reported that Aeromonas is capable of causing ocular infection leading to inflammation and abscesses [60,61]. Notably, several antiviral-related genes (irf3, mda5, and rig-i) have been identified as key players in antimicrobial immune responses and the maintenance of microbial homeostasis [62][63][64]. Therefore, Aeromonas may contribute significantly to the proinflammatory response observed in trout OM at 4 DPI after IHNV infection.…”

Section: Discussionmentioning

confidence: 99%

Ocular mucosal homeostasis of teleost fish provides insight into the coevolution between microbiome and mucosal immunity

Kong,

Cheng,

Cao

et al. 2024

Microbiome

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Background The visual organ plays a crucial role in sensing environmental information. However, its mucosal surfaces are constantly exposed to selective pressures from aquatic or airborne pathogens and microbial communities. Although few studies have characterized the conjunctival-associated lymphoid tissue (CALT) in the ocular mucosa (OM) of birds and mammals, little is known regarding the evolutionary origins and functions of immune defense and microbiota homeostasis of the OM in the early vertebrates. Results Our study characterized the structure of the OM microbial ecosystem in rainbow trout (Oncorhynchus mykiss) and confirmed for the first time the presence of a diffuse mucosal-associated lymphoid tissue (MALT) in fish OM. Moreover, the microbial communities residing on the ocular mucosal surface contribute to shaping its immune environment. Interestingly, following IHNV infection, we observed robust immune responses, significant tissue damage, and microbial dysbiosis in the trout OM, particularly in the fornix conjunctiva (FC), which is characterized by the increase of pathobionts and a reduction of beneficial taxa in the relative abundance in OM. Critically, we identified a significant correlation between viral-induced immune responses and microbiome homeostasis in the OM, underscoring its key role in mucosal immunity and microbiota homeostasis. Conclusions Our findings suggest that immune defense and microbiota homeostasis in OM occurred concurrently in early vertebrate species, shedding light on the coevolution between microbiota and mucosal immunity.

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Section: Discussionmentioning

confidence: 99%

Ocular mucosal homeostasis of teleost fish provides insight into the coevolution between microbiome and mucosal immunity

Kong,

Cheng,

Cao

et al. 2024

Microbiome

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“…This protein is activated by TLRs during the immune response (Shan et al, 2018) and regulates gene expression in response to cytokines, stress, and bacterial and viral infections (Kim et al, 2014), and notably the IFN regulatory factors (IRFs, particularly IRF3, IRF4 and IRF8). The IRFs are key regulators of innate antiviral and antibacterial responses in vertebrates including fish (Clark et al, 2021; Han et al, 2023). The observed ontology enrichment clustering of these TFs reinforces the similarities between the conditions in our study (Figure 7, Supplementary Figure 2).…”

Section: Discussionmentioning

confidence: 99%

Multiomics uncovers the epigenomic and transcriptomic response to viral and bacterial stimulation in turbot

Aramburu,

Gómez-Pardo,

Rodríguez-Villamayor

et al. 2024

Preprint

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Uncovering the epigenomic regulation of immune responses is essential for a comprehensive understanding of host defence mechanisms but remains poorly described in farmed fish. Here, we report the first annotation of the innate immune regulatory response in the genome of turbot (Scophthalmus maximus), a farmed flatfish. We integrated RNA-Seq with ATAC-Seq and ChIP-Seq (histone marks H3K4me3, H3K27ac and H3K27me3) using samples from head kidney. Sampling was performed 24 hours post-stimulation with viral (poly I:C) and bacterial (inactivate Vibrio anguillarum) mimics in vivo and in vitro (primary leukocyte cultures). Among the 8,797 differentially expressed genes (DEGs), we observed enrichment of transcriptional activation pathways in response to Vibrio and immune response pathways - including interferon stimulated genes - for poly I:C. Meanwhile, metabolic and cell cycle were downregulated by both mimics. We identified notable differences in chromatin accessibility (20,617 in vitro, 59,892 in vivo) and H3K4me3 bound regions (11,454 in vitro, 10,275 in vivo) - i.e. marking active promoters - between stimulations and controls. Overlaps of DEGs with promoters showing differential accessibility or histone mark binding revealed a significant coupling of the transcriptome and chromatin state. DEGs with activation marks in their promoters were enriched for similar functions to the global DEG set, but not in all cases, suggesting key regulatory genes were in poised or bivalent states. Active promoters and putative enhancers were differentially enriched in transcription factor binding motifs, many of them common to viral and bacterial responses. Finally, an in-depth analysis of immune response changes in chromatin state surrounding key DEGs encoding transcription factors was performed. This comprehensive multi-omics investigation provides an improved understanding of the epigenomic basis for the turbot immune responses and provides novel functional genomic information that can be leveraged in selective breeding towards enhanced disease resistance.

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Characterization of Japanese flounder (Paralichthys olivaceus) STAT members: An immune-related gene family involved in Edwardsiella tarda and temperature stress

Chen

Han

Liu

et al. 2023

Fish & Shellfish Immunology

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Characterization and expression analysis of the interferon regulatory factor (IRF) gene family in zig-zag eel (Mastacembelus armatus) against Aeromonas veronii infection

Cited by 5 publications

References 55 publications

Ocular mucosal homeostasis of teleost fish provides insight into the coevolution between microbiome and mucosal immunity

Ocular mucosal homeostasis of teleost fish provides insight into the coevolution between microbiome and mucosal immunity

Multiomics uncovers the epigenomic and transcriptomic response to viral and bacterial stimulation in turbot

Characterization of Japanese flounder (Paralichthys olivaceus) STAT members: An immune-related gene family involved in Edwardsiella tarda and temperature stress

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