Characterization and expression analysis of AcSERK2, a somatic embryogenesis and stress resistance related gene in pineapple

Ma, Jun; He, Yehua; Hu, Zhongyi; Xu, Weihong; Xia, Jingxian; Guo, Chunyan; Lin, Shunquan; Cao, Li; Chen, Chengjie; Cheng-hou, WU; Zhang, Junli

doi:10.1016/j.gene.2012.03.013

Cited by 32 publications

(25 citation statements)

References 44 publications

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“…Consequently, the restriction of PeSERK expression to the early developmental stages of SE and to proembryogenic zones suggests that PeSERK is involved in the acquisition of competence but not in later stages of SE. This pattern was also observed in Ananas comosus, for which AcSERK showed a weak expression signal during later stages of SE (Ma et al 2012). The same pattern was reported for the SE systems of Dactylis glomerata (Schmidt et al 1997), Cocos nucifera (Pérez-Nuñez et al 2009) and Cyclamen persicum (Savona et al 2012).…”

Section: Discussionsupporting

confidence: 76%

“…Throughout this process, SERK expression is not restricted to one particular cell type but occurs in morphologically distinct single cells and small cell clusters. This expression continues through proembryogenic mass formation until the embryos are at the globular stage (Schmidt et al 1997;Ma et al 2012;Ma et al 2014). Furthermore, somatic embryos of different species arise from morphologically distinct cell types that express SERK genes.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, somatic embryos of different species arise from morphologically distinct cell types that express SERK genes. For example, enlarged and vacuolated cells that are derived from the procambium and express SERK were embryogenically competent in carrot (Schmidt et al 1997), but in pineapple, the proliferating embryogeniccompetent cells were small, isodiametric, non-vacuolated and cytoplasm rich and expressed AcSERK2 and AcSERK3 (Ma et al 2012;Ma et al 2014).…”

Section: Introductionmentioning

confidence: 99%

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Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

et al. 2015

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The integration of cellular and molecular data is essential for understanding the mechanisms involved in the acquisition of competence by plant somatic cells and the cytological changes that underlie this process. In the present study, we investigated the dynamics and fate of Passiflora edulis Sims cotyledon explants that were committed to somatic embryogenesis by characterizing the associated ultrastructural events and analysing the expression of a putative P. edulis ortholog of the Somatic Embryogenesis Receptor-like Kinase (SERK) gene. Embryogenic calli were obtained from zygotic embryo explants cultured on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Callus formation was initiated by the division of cells derived from the protodermal and subprotodermal cells on the abaxial side of the cotyledons. The isodiametric protodermal cells of the cotyledon explants adopted a columnar shape and became meristematic at the onset of PeSERK expression, which was not initially detected in explant cells. Therefore, we propose that these changes represent the first observable steps towards the acquisition of a competent state within this regeneration system. PeSERK expression was limited to the early stages of somatic embryogenesis; the expression of this gene was confined to proembryogenic zones and was absent in the embryos after the globular stage. Our data also demonstrated that the dynamics of the mobilization of reserve compounds correlated with the differentiation of the embryogenic callus.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

et al. 2015

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“…Somatic embryogenesis is a highly efficient means of propagating pineapples and can be induced in this species by exposure to 2,4-dichlorophenoxyacetic acid (2,4-D), with the frequency of somatic embryo induction reaching 95%; the number of somatic embryos per callus (3×3×3 mm) after 40 days (d) of culture is ∼48 (He et al , 2007). The origin of SE in pineapple is unicellular (He et al , 2010; Ma et al , 2012a,b). Somatic embryogenesis thus provides a good way to genetically transform pineapple.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro

et al. 2014

Genet. Mol. Biol.

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Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20–80 fold more than in anther but similar to that of non-embryogenic callus, which was 20–50 fold that of anther. AcSERK3 expression in root was 80 fold higher than in anther and the highest amongst all organs tested. These results indicate that AcSERK3 plays an important role in callus proliferation and root development. His-tagged AcSERK3 protein was successfully expressed and the luminescence of His6-AcSERK3 protein was only ∼5% of that of inactivated AcSERK3 protein and reaction buffer without protein, and 11.3% of that of an extract of host Escherichia coli pET-30a. This finding confirmed that the AcSERK3 fusion protein had autophosphorylation activity.

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“…The increase of alkaloids may be due to perceived stress signals, created by NaCl (at low levels) activates signal transduction pathways by promoting transcriptional activation of cascade genes like SOS1, somatic embryogenesis receptorlike kinase (SERK) whose expression are up-regulated in embryogenenic calli (Hu et al 2005;Zhang et al 2010;Elmaghrabi et al 2013). Molecular mechanisms reveal that the coordinated expression of genes (stress-related-, somatic embryogenesis related-and biosynthetic genes), often at its maximum in stress (NaCl amended) conditions, emerges as a major regulating mechanism for over accumulation of secondary metabolites (Nimchuk et al 2003;Dutta et al 2007;Ma et al 2012;Elmaghrabi et al 2013). The molecular role of these encoded proteins though not known fully NaCl-mediated enhancement would be a good approach in secondary metabolite synthesis.…”

Section: Discussionmentioning

confidence: 99%

NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities

Fatima

Mujib

Tonk

2015

Plant Cell Tiss Organ Cult

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An improved synthesis of vinblastine and vincristine was observed in Catharanthus roseus L. (G). Don by using NaCl as an elicitor. Various in vitro grown embryogenic tissues were cultivated under salinity stress for enhanced synthesis of alkaloids. Different levels of salt [control (0 mM), NT1 (25 mM), NT2 (50 mM), NT3 (75 mM), NT4 (100 mM), and NT5 (125 mM)] were amended in MS and callus biomass growth (fresh-and dryweight) and biochemical attributes at various embryogenic stages were studied. Maximum callus biomass reduction was observed in 125 mM NaCl amended medium. Antioxidant enzymes i.e. superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase and glutathione reductase activities were assayed as in vitro grown tissues were elicitated with NaCl, causing cellular stress. The antioxidant enzymes activity increased linearly with increasing NaCl level in medium, 4.97 EU min -1 mg -1 SOD and 3.14 EU min -1 mg -1 CAT, both being maximum in proliferating embryos at NT5. Quantitative estimation and comparative yield of alkaloids were made in response to NaCl stress in different cultivated tissues by using HPTLC method. Vinblastine content was observed to be maximum in regenerated leaves (14.17 mg/g dry wt) on 25 mM NaCl amended medium, followed by in vitro raised shoots. Similarly, better accumulation of vincristine (5.12 mg/g dry wt) was also noted in NaCl amended medium especially at low level (NT1). The data presented indicate that the synthesis of Catharanthus alkaloids was growth specific and was influenced by NaCl levels.

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Characterization and expression analysis of AcSERK2, a somatic embryogenesis and stress resistance related gene in pineapple

Cited by 32 publications

References 44 publications

Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

Cellular and molecular changes associated with competence acquisition during passion fruit somatic embryogenesis: ultrastructural characterization and analysis of SERK gene expression

Characterization of the third SERK gene in pineapple (Ananas comosus) and analysis of its expression and autophosphorylation activity in vitro

NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities

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