Characterization and expression analysis of a goose-type lysozyme from the rock bream Oplegnathus fasciatus, and antimicrobial activity of its recombinant protein

Identification of a myeloperoxidase-like ortholog from rock bream (Oplegnathus fasciatus), deciphering its transcriptional responses to induced pathogen stress

“…The immune challenge experiments were carried out as previously described [22], and at least 3 animals were killed for tissue collection from each challenge group at each time point. Thereafter, gill tissues and spleen tissues were collected as described in section 2.2.…”

Genomic characterization, phylogeny and gene regulation of g-type lysozyme in sole (Solea senegalensis)

“…Several studies have demonstrated that mRNA levels are up-regulated after challenge with Gram-negative [5,10,13,14,18,19] and Gram-positive [19] bacteria. About PAMPs, the lipopolysaccharide (LPS), a major component of the cell wall of gram-negative bacteria, can also upregulate expression of g-type lysozyme [14,18,19]. Recently, it has been described that polyinosinic:polycytidylic acid (Poly(I:C)), a dsRNA that mimic viral infections, can also act as an inducer of gtype lysozyme gene expression [19].…”

“…About PAMPs, the lipopolysaccharide (LPS), a major component of the cell wall of gram-negative bacteria, can also upregulate expression of g-type lysozyme [14,18,19]. Recently, it has been described that polyinosinic:polycytidylic acid (Poly(I:C)), a dsRNA that mimic viral infections, can also act as an inducer of gtype lysozyme gene expression [19]. However, little information is available about the effects of other PAMPs.…”

Molecular characterization and expression pattern of c-type and g-type lysozyme isoforms in starry flounder Platichthys stellate infected with Streptococcus parauberis

“…In the alignment, Glu 72 and Asp 96 (starry flounder numbering) are conserved in all species, whereas the Asp 85 residue is present in all teleostean sequences except common carp Cyprinus carpio [5,8,[33][34][35]. Unlike its c-type counterpart, starry flounder g-type lysozyme has no potential cysteine residues capable of disulfide bridging, which is congruent with most other piscine g-type orthologues [4,5,7,8,[33][34][35][36]. G-type lysozyme from this species may function in an intracellular environment as evidenced by the absence of signal peptide, which is also in agreement with other teleostean orthologues.…”