Characterization and Evaluation The Biological Activity of Prepared Nano-Gentamicin Nanoparticles

Abstract

Nanotechnology is a capable approach to enhance the activity of common antimicrobial agent mainly used in human and veterinary drug. Gentamycin is a broad-spectrum antibacterial against Gram-positive and Gram-negative bacteria. The study aims to determine the characterization and the biological activity of gentamicin in the Nano-form prepared by Sol-gel application using an ultrasound device by aqueous solution at temperature (80)0C. Nano gentamycin examined by Scanning electron microscopy (SEM), transmission (TEM), atomic force microscopy (AFM) and antibacterial effect of both Nano and stander gentamycin were analyzed. The results showed that the average size of gentamycin nanoparticles was 68.51 nanometers with homogeneous distribution. The EDX showed large number of elements present in the Nano solution. The antibacterial effect of Nano gentamycin took a wide inhibition range of Gram-positive and Gram-negative bacteria compared to the standard substance, where the inhibition diameter for E.coli was 29 mm, and for St. epidermidis was 27 mm. The MIC of gentamicin nanostructures was the concentration 0.00125 mg/ml.