Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi

Dimkić, Ivica; Živković, Svetlana; Berić, Tanja; Ivanović, Žarko; Gavrilović, Veljko; Stanković, Slaviša; Fira, Djordje

doi:10.1016/j.biocontrol.2013.03.012

Cited by 98 publications

(53 citation statements)

References 33 publications

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“…Our findings demonstrated that strain MBCU2 produced some multifarious allelochemicals (such as lipopeptides), which proved to be effective in controlling M. phaseolina growth. Antifungal activities have been reported for many different lipopeptides . Similar results also reported by Arrebola et al .…”

Section: Discussionsupporting

confidence: 89%

Isolation and identification of allelochemicals produced by B. sonorensis for suppression of charcoal rot of Arachis hypogaea L.

Pandya

Saraf

2014

J. Basic Microbiol.

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Bacillus sonorensis MBCU2 isolated from vermicompost-amended soil from Gujarat, India showed most antagonistic activity against Macrophomina phaseolina by dual culture screening. The culture supernatant of MBCU2 completely suppressed the mycelia growth of pathogen, indicating that suppression was due to the presence of allelochemicals in the culture filtrate. Results of scanning electron microscopy revealed that MBCU2 caused morphological alteration in mycelia of M. phaseolina as evident by hyphal lysis and perforation. Lipopeptides (iturin A and surfactin) produced by MBCU2 were detected and identified by MALDI-TOF-MS as well as liquid chromatography coupled with ESI-MS/MS. Pot trial studies conducted by seed bacterization with MBCU2 resulted in statistically significant increase in Arachis hypogaea L. vegetative growth parameters such as root length (91%), shoot length (252%), fresh weight (71%), dry weight (57%), number of pod (128%), and number of seed (290%) in M. phaseolina infested soil over control as well as decreased M. phaseolina disease severity. We suggest that allelochemicals production can be linked to the mechanism of protection of A. hypogaea L. from M. phaseolina by B. sonorensis MBCU2.

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Section: Discussionsupporting

confidence: 89%

Isolation and identification of allelochemicals produced by B. sonorensis for suppression of charcoal rot of Arachis hypogaea L.

Pandya

Saraf

2014

J. Basic Microbiol.

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“… and Dimkić et al . , who also report that, multiple antifungal peptide genes present in antagonistic Bacillus species. Alternatively, antifungal compound produced by antagonist may be playing important mechanism during pathogen suppression.…”

Section: Discussionmentioning

confidence: 88%

“…Among these mechanisms, antibiosis by means of antimicrobial peptides (AMPs) produced by Bacillus spp. have been implicated in the biocontrol of several plant pathogens [12][13][14][15]. AMPs include cyclic lipopeptides such as bacillomycin, fengycin, iturin, and surfactin has been linked to the presence of the AMP biosynthetic genes such as bmyB, fenD, ituC, and srfAA [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Characterization of antagonistic‐potential of two Bacillus strains and their biocontrol activity against Rhizoctonia solani in tomato

Solanki

Singh

Srivastava

et al. 2013

J. Basic Microbiol.

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To investigate the biocontrol mechanism of two antagonistic Bacillus strains (Bacillus subtilis MB14 and Bacillus amyloliquefaciens MB101), three in vitro antagonism assays were screened and the results were concluded that both strains inhibited Rhizoctonia solani growth in a similar manner by dual culture assay, but the maximum percent of inhibition only resulted with MB101 by volatile and diffusible metabolite assays. Moreover, cell free supernatant (CFS) of MB101 also showed significant (p > 0.05) growth inhibition as compared to MB14, when 10 and 20% CFS mix with the growth medium of R. solani. After in vitro-validation, both strains were evaluated under greenhouse and the results concluded that strain MB101 had significant biocontrol potential as compared to MB14. Strain MB101 was enhanced the plant height, biomass and chlorophyll content of tomato plant through a higher degree of root colonization. In field trials, strain MB101 showed higher lessening in root rot symptoms with significant fruit yield as compare to strain MB14 and infected control. Next to the field study, the presence of four antibiotic genes (srfAA, fenD, ituC, and bmyB) also concluded the antifungal nature of both Bacillus strains. Phylogenetic analysis of protein sequences revealed a close relatedness of three genes (srfAA, fenD, and ituC) with earlier reported sequences of B. subtilis and B. amyloliquefaciens. However, bmyB showed heterogeneity in among both strains (MB14 and MB101) and it may be concluded that higher degree of antagonism, root colonization and different antibiotic producing genes may play an important role in biocontrol mechanism of strain MB101.

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“…hungaricus was determined against seven bacterial strains ( Aeromonas hydrophila ATCC 49140, Pseudomonas aeruginosa ATCC 15442, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Methicillin resistant Staphylococcus aureus (MRSA) ATCC 33591, Listeria monocytogenes 1/2a ATCC 19111, Bacillus subtilis ATCC 6633 and Xanthomonas arboricola ) and eight species of fungi ( Aspergillus flavus , Aspergillus niger , Fusarium subglutinans , Fusarium semitectum , Fusarium equiseti , Penicillium sp., Gliocladium roseum and Chaetomium sp.). The reference strains of bacteria used in this study were obtained from the American Type Culture Collection (ATCC) and from existing laboratory collections, except for the Xanthomonas arboricola strain, which was isolated from walnut fruit previously [13]. The bacterial strains were cultured in MHB and MHA (Müller-Hinton broth and agar, HiMedia, Mumbai, India), with the exception of L .…”

Section: Methodsmentioning

confidence: 99%

Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae)

et al. 2016

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The chemical defence of the millipede Pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. In total, independently of sex, 44 compounds were identified. All compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. The relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. The two dominant quinones in both sexes were 2-methyl-1,4,-benzoquinone and 2-methoxy-3-methyl-1,4-benzoquinone. Antibacterial and antifungal activity of the defensive secretion was evaluated in vitro against seven bacterial strains and eight fungal species. With the aid of a dilution technique, the antimicrobial potential of the secretion and high sensitivity of all tested strains were confirmed. The lowest minimum concentrations of these compounds (0.20–0.25 mg/mL) were sufficient for inhibition of Aeromonas hydrophila, Listeria monocytogenes and Methicillin resistant Staphylococcus aureus (MRSA). The growth of eight tested fungal species was inhibited by slightly lower concentrations of the secretion, with Fusarium equiseti as the most sensitive fungus and Aspergillus flavus as the most resistant. Values of MIC and MFC in the employed microdilution assay ranged from 0.10 to above 0.35 mg/mL. The given extract contains antimicrobial components potentially useful as therapeutic agents in the pharmaceutical and agricultural industries.

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Characterization and evaluation of two Bacillus strains, SS-12.6 and SS-13.1, as potential agents for the control of phytopathogenic bacteria and fungi

Cited by 98 publications

References 33 publications

Isolation and identification of allelochemicals produced by B. sonorensis for suppression of charcoal rot of Arachis hypogaea L.

Isolation and identification of allelochemicals produced by B. sonorensis for suppression of charcoal rot of Arachis hypogaea L.

Characterization of antagonistic‐potential of two Bacillus strains and their biocontrol activity against Rhizoctonia solani in tomato

Chemical Defence in a Millipede: Evaluation and Characterization of Antimicrobial Activity of the Defensive Secretion from Pachyiulus hungaricus (Karsch, 1881) (Diplopoda, Julida, Julidae)

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