Characterization and Evaluation of Cell-Penetrating Activity of Brevinin-2R: An Amphibian Skin Antimicrobial Peptide

Nooranian, Samin; Oskuee, Reza Kazemi; Jalili, Amin

doi:10.1007/s12033-021-00433-5

Cited by 4 publications

(3 citation statements)

References 39 publications

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“…Brevinin-2R is an antimicrobial peptide from the cutaneous secretion of Rana ridibunda that has a membrane-disruptive mode of action. The results demonstrated that brevinine inhibited the proliferation of 50% of HeLa cells at ~80 μM [ 66 ]. Another example is aurein peptides 1.2 and 3.1 from Litoria sp., which showed activity against the NCI cancer cell line, with LC 50 values of 4 and 10 μM, respectively [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa

Silva

Guindo

Oliveira

et al. 2023

Toxins

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In recent years, antimicrobial peptides isolated from amphibian toxins have gained attention as new multifunctional drugs interacting with different molecular targets. We aimed to rationally design a new peptide from temporin-PTa. Hp-MAP3 (NH2-LLKKVLALLKKVL-COOH), net charge (+4), hydrophobicity (0.69), the content of hydrophobic residues (69%), and hydrophobic moment (0.73). For the construction of the analog peptide, the physicochemical characteristics were reorganized into hydrophilic and hydrophobic residues with the addition of lysines and leucines. The minimum inhibitory concentration was 2.7 to 43 μM against the growth of Gram-negative and positive bacteria, and the potential for biofilm eradication was 173.2 μM. Within 20 min, the peptide Hp-MAP3 (10.8 μM) prompted 100% of the damage to E. coli cells. At 43.3 μM, eliminated 100% of S. aureus within 5 min. The effects against yeast species of the Candida genus ranged from 5.4 to 86.6 μM. Hp-MAP3 presents cytotoxic activity against tumor HeLa at a concentration of 21.6 μM with an IC50 of 10.4 µM. Furthermore, the peptide showed hemolytic activity against murine erythrocytes. Structural studies carried out by circular dichroism showed that Hp-MAP3, while in the presence of 50% trifluoroethanol or SDS, an α-helix secondary structure. Finally, Amphipathic Hp-MAP3 building an important model for the design of new multifunctional molecules.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa

Silva

Guindo

Oliveira

et al. 2023

Toxins

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show abstract

“…The validation of 3D homology model was carried out by PROCHECK stereo-chemical assessment through quality factor, Ramachandran plot and residual properties of constructed model. The dihedral angles φ against ψ of possible conformations of amino acids in protein structure had also been studied in Ramachandran plot [41]. SAVES (Structure Validation Server: https://saves.mbi.ucla.edu/) web server was used to know the probable structural errors and zscore [41].…”

Section: Nnat Genementioning

confidence: 99%

“…The dihedral angles φ against ψ of possible conformations of amino acids in protein structure had also been studied in Ramachandran plot [41]. SAVES (Structure Validation Server: https://saves.mbi.ucla.edu/) web server was used to know the probable structural errors and zscore [41]. Secondary structure properties of NNAT protein was investigated through the α-helices, identi cation of trans-membrane region and low complexity region [34].…”

Section: Nnat Genementioning

confidence: 99%

In silico identification of the rare-coding pathogenic mutations and structural modeling of human NNAT gene associated with anorexia nervosa

et al. 2022

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Purpose Increase susceptibility towards Anorexia nervosa (AN) was reported with reduced levels of NNAT gene. We sought to investigate the most pathogenic rare-coding missense mutations (nsSNPs) of NNAT and their potential damaging impact on protein function through transcript level sequence and structure based in silico approaches. Methods Gene sequence, SNPs of NNAT was retrieved from public databases and the putative post-translational modi cation (PTM) sites were analyzed. Distinctive in silico algorithms were recruited for transcript level SNPs analyses and to characterized high risk rare-coding nsSNPs along with their impact on protein stability function. Ab initio 3D-modeling of wild-type, alternate model prediction for most deleterious nsSNP, validation and recognition of druggable binding pockets were also performed. AN 3D therapeutic compounds followed rule of drug-likeness were docked with most pathogenic variant of NNAT to estimate the drugs' binding free energies.Results Conclusively, 10 transcripts (201-205) based nsSNPs from 3 rare-coding missense variants i.e., rs539681368, rs542858994, rs560845323 out of 840 exonic SNPs were identi ed. Transcript based functional impact analyses predicted rs539681368 (C30Y) from NNAT-204 as the high risk rare-coding pathogenic nsSNP, deviating protein functions. The 3D-modeling analysis of AN drugs' binding energies indicated lowest binding free energy (ΔG) and signi cant inhibition constant (K i ) with mutant models C30Y.Conclusions Mutant model (C30Y) exhibiting signi cant drug binding a nity and the commonest interaction observed at the acetylation site K59. Thus, based on these ndings, we concluded that the identi ed nsSNP may serve as potential targets for various studies, diagnosis and therapeutic interventions. Level of evidence No level of evidenceWhat Is Already Know On The Subject? Lombardi et al., 2019 and Ceccarrini et al in 2021 reported the role of con rmation of the involvement of the NNAT gene in the pathogenesis of AN. Further, its role in energy metabolic homeostasis has been clearly established through a vast spectrum of literature. What your study adds?Transcript level predictions of rare-coding high risk pathogenic human NNAT gene's SNPs by different in silico ML algorithms and their functional impact on protein stability. Characterizing the structural inference of rare-coding deleterious missense mutations and computing the molecular binding energies of most pathogenic 3D model of NNAT variant with therapeutic compounds of AN.

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Protein Delivery and Mimicry

Langel

2023

CPP, Cell-Penetrating Peptides

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Characterization and Evaluation of Cell-Penetrating Activity of Brevinin-2R: An Amphibian Skin Antimicrobial Peptide

Cited by 4 publications

References 39 publications

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa

Evaluation of the Synthetic Multifunctional Peptide Hp-MAP3 Derivative of Temporin-PTa

In silico identification of the rare-coding pathogenic mutations and structural modeling of human NNAT gene associated with anorexia nervosa

Protein Delivery and Mimicry

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