Characterization and evaluation of a new triosephosphate isomerase homologue from Haemaphysalis longicornis as a candidate vaccine against tick infection

Liang, Na; Dong, Hongmeng; Fan, Xiang-Yuan; Wu, Ya-Xue; Yang, Feng; Liu, Xiaoya; Hu, Yonghong

doi:10.1016/j.ttbdis.2022.101968

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…The antibodies against iron‐binding proteins ferritin 1 and ferritin 2 from H. longicornis conferred 34% and 49% protection against H. longicornis , respectively 58 . The antibodies against recombinant triosephosphate isomerase from H. longicornis exhibited 50.9% efficacy against its infestation 39 . Amongst DNA vaccines against H. longicornis , lipocalins DNA vaccine exhibited 30% immune protection efficiency in immunized rabbits, 10 whereas paramyosin DNA vaccine exhibited an overall efficacy of 50% 11 .…”

Section: Discussionmentioning

confidence: 99%

“…38 The purified protein was separated using 10% SDS-PAGE and transferred onto a polyvinylidene fluoride membrane. After blocking with 5% skimmed milk, the membranes were exposed to rabbit anti-H. longicornis serum derived from our previous study 39 or rabbit negative serum (1:2000) for 1 h at room temperature (RT). After washing three times with PBS-Tween-20 (PBST), the membranes were exposed to horseradish peroxidase-conjugated goat antirabbit immunoglobulin (Ig)G (1:2000) for 2 h at RT.…”

Section: Vaccine Formulation 241 Expression and Purification Of The R...mentioning

confidence: 99%

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Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks

Wang

et al. 2023

Pest Management Science

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Background Haemaphysalis longicornis is an obligate hematophagous ectoparasite, which transmits various pathogens to humans, livestock and wild animals. Hexokinase (HK) is a key regulatory enzyme of the glycolytic pathway in the organisms. However, little is known about hexokinase and its functions in ticks. Results The open reading frame of the H. longicornis HK (HlHK) gene was 1425 bp and encoded a protein of 474 amino acids, containing conserved domains for glucose, glucose 6‐phosphate, and adenosine triphosphate. The expression of HlHK gene was detected at different developmental stages and in different tissues of unfed female ticks. Enzyme‐linked immunosorbent assay revealed that both HK protein‐ and DNA‐based vaccines increased the antibody levels of the immunized animals. A vaccination trail on rabbits against H. longicornis infestation indicated that the rHlHK protein and HlHK DNA vaccines reduced the number of attached female ticks by 9% and 12%, egg mass weight by 36% and 34%, and egg hatching rate by 41% and 17%, respectively. Overall, protein vaccination conferred 65.6% protection against adult female ticks, whereas the DNA vaccine conferred 51.8% protection. Conclusion This is the first report of the molecular characterization of the HK protein and sequencing of the HK gene from H. longicornis. Positive results from vaccination trials on rabbits of the recombinant HK protein and HK DNA suggest that these novel anti‐tick vaccines potentially can be used as viable tick control tools for the management of the Asian longhorned tick. Additionally, inhibition of glucose metabolism may be a new strategy for tick control. © 2023 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 99%

Section: Vaccine Formulation 241 Expression and Purification Of The R...mentioning

confidence: 99%

Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks

Wang

et al. 2023

Pest Management Science

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“…This is not because it presents a ground-breaking discovery, but rather because it is a robust collection of evidence supporting the different research paths being followed in the pursuit of effective tick and tick-borne pathogens vaccines. Since then, at least 68 different “exposed” and/or “concealed” antigens have been tested in vaccination trials against different tick species aiming to achieve a reduction in tick infestation ( Table 1 ) [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. The attractive idea of transmission blocking vaccines has also thrived, and some studies have included the hypothesis that impairing a tick targeted antigen could also affect the pathogen life cycle occurring within its vector ( Table 1 ).…”

Section: Impactmentioning

confidence: 99%

Tick Vaccines and Concealed versus Exposed Antigens

Antunes

Domingos

2023

Pathogens

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Anti-tick vaccines development mainly depends on the identification of suitable antigens, which ideally should have different features. These should be key molecules in tick biology, encoded by a single gene, expressed across life stages and tick tissues, capable of inducing B and T cells to promote an immunological response without allergenic, hemolytic, and toxic effects; and should not be homologous to the mammalian host. The discussion regarding this subject and the usefulness of “exposed” and “concealed” antigens was effectively explored in the publication by Nuttall et al. (2006). The present commentary intends to debate the relevance of such study in the field of tick immunological control.

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“…Rabbits are currently used as a model organism in anti-tick vaccines assays against ticks of the genera Amblyomma , Dermacentor , Hyalomma , Haemaphysalis , Ixodes , Ornithodoros , and Rhipicephalus ( Figure 1 ) [ 23 , 35 , 63 , 64 , 65 , 66 ]. The following paragraphs will discuss the main trials carried out on rabbits for anti-tick vaccine development.…”

Section: Vaccination In Rabbitsmentioning

confidence: 99%

Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario

Rodríguez-Durán,

Ullah,

Parizi

et al. 2023

Pathogens

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Studies evaluating candidate tick-derived proteins as anti-tick vaccines in natural hosts have been limited due to high costs. To overcome this problem, animal models are used in immunization tests. The aim of this article was to review the use of rabbits as an experimental model for the evaluation of tick-derived proteins as vaccines. A total of 57 tick proteins were tested for their immunogenic potential using rabbits as models for vaccination. The most commonly used rabbit breeds were New Zealand (73.8%), Japanese white (19%), Californians (4.8%) and Flemish lop-eared (2.4%) rabbits. Anti-tick vaccines efficacy resulted in up to 99.9%. Haemaphysalis longicornis (17.9%) and Ornithodoros moubata (12.8%) were the most common tick models in vaccination trials. Experiments with rabbits have revealed that some proteins (CoAQP, OeAQP, OeAQP1, Bm86, GST-Hl, 64TRP, serpins and voraxin) can induce immune responses against various tick species. In addition, in some cases it was possible to determine that the vaccine efficacy in rabbits was similar to that of experiments performed on natural hosts (e.g., Bm86, IrFER2, RmFER2, serpins and serine protease inhibitor). In conclusion, results showed that prior to performing anti-tick vaccination trials using natural hosts, rabbits can be used as suitable experimental models for these studies.

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Characterization and evaluation of a new triosephosphate isomerase homologue from Haemaphysalis longicornis as a candidate vaccine against tick infection

Cited by 5 publications

References 33 publications

Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks

Molecular characterization of hexokinase (HK) in Haemaphysalis longicornis and evaluation of HK protein‐ and DNA‐based vaccines against adult ticks

Tick Vaccines and Concealed versus Exposed Antigens

Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario

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