Characterization and DNA sequence of the b6w2 allotype of the rabbit immunoglobulin kappa 1 light chain (b locus)

Loo, W. van der; Bouton, Christianne; Sánchez, María Teresa; Mougel, Florence; Castién, Enrique; Hamers, R.; Monnerot, M.

doi:10.1007/bf00179394

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…Genotypes were inferred from the phenotypic arrays (Table 1; cf. van der Loo et al 1991Loo et al ,1995Loo et al ,1998. Sera with phenotypes that could not readily be resolved into their putative allelic components were subjected to double gel immunodiffusion (Kelus and Steinberg 1991), a technique which allows one to verify whether determinants recognized by two different antibodies are epitopes of the same or of different molecular species in the test serum.…”

Section: Igkc1 Typingmentioning

confidence: 99%

“…The b6 allotype occurs in areas of type A as well as in areas of type B prevalence, often together with the b6w2 allotype. DNA sequence comparisons have shown that the b6w2 gene derives from the b6 gene lineage (van der Loo et al 1995). The b6 lineage is also present in RAD, implying the coalescence of the b6 genes of the major mtDNA lineages.…”

Section: Relevance To Igkc1-locus Evolutionary Patternsmentioning

confidence: 99%

“…Disulphide bonds between Ig domains of a same polypeptide chain have never been observed for any of the numerous members of the Ig protein family, for any species (Halaby and Mornon 1998). This bond might contribute to the unusual degree of allelic variation (Ayadi et al 1991;McCartney-Francis 1984;van der Loo et al 1995), but it is also possible that the emergence of the interdomain bond was primarily a consequence, rather than a cause, of the particular evolutionary dynamics at this locus.…”

Section: Interdomain Bond: Cause or Effect?mentioning

confidence: 99%

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Cytonuclear disequilibria in wild populations of rabbit ( Oryctolagus cuniculus L. ) suggest unequal allele turnover rates at the b locus ( IGKC1 )

Loo¹,

Mougel

Sánchez

et al. 1999

Immunogenetics

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DNA sequence comparisons suggest that evolutionary rates at the rabbit IGKC1 locus can differ among allelic lineages. Here we address the question of whether population turnover rates can vary among IGKC1 alleles. We studied the distribution of sixteen IGKC1 (or b-locus) allotypes in areas comprising the aboriginal species range (Iberian peninsula). Rabbits in this area belong to one of two distantly related mitochondrial lineages (mtDNA types) A and B. In the more recent distribution area of the species, all rabbits belong to the mtDNA type B lineage, and IGKC1 alleles b4 and b5 comprise over 90% of the gene pool. These two alleles are also predominant in areas of mtDNA type B prevalence within the Iberian range. However, in areas of mtDNA type A prevalence, the b4 and b5 allotypes are rare or absent; they apparently have been replaced by serologically related, but distinct, 'endemic' variants. The cytonuclear disequilibria were highly significant, also within the subsample consisting of populations from Spain. These observations suggest that allelic persistence times for the predominant IGKC1 lineages could be shorter than the divergence time of the major mtDNA lineages A and B. In contrast, the relative gene frequencies of the IGKC1 allele b9 were similar among the type A and type B rabbits; it was present in most populations at low frequency. In consequence, persistence times of the b9 allele appear to be longer than the divergence time of lineages A and B. The data reported here are in agreement with the DNA sequence data, providing further proof that the molecular clock can run at different rates among allelic lineages at the rabbit IGKC1 locus.

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Section: Igkc1 Typingmentioning

confidence: 99%

Section: Relevance To Igkc1-locus Evolutionary Patternsmentioning

confidence: 99%

Section: Interdomain Bond: Cause or Effect?mentioning

confidence: 99%

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Cytonuclear disequilibria in wild populations of rabbit ( Oryctolagus cuniculus L. ) suggest unequal allele turnover rates at the b locus ( IGKC1 )

Loo¹,

Mougel

Sánchez

et al. 1999

Immunogenetics

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Diversity and evolutionary history of the MHC DQA gene in leporids

et al. 2008

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The European rabbit (Oryctolagus cuniculus) is used as a model for many human diseases, yet comparatively little is known of its genetics, particularly at important loci such as the major histocompatibility complex (MHC). This study investigated genetic diversity and evolutionary history of the DQA gene in a range of leporid species by analysing coding sequence diversity of exon 2 and intron 2 in 53 individuals of 16 different species. Fifty leporid DQA alleles were detected, including 13 novel European rabbit alleles. In the rabbit, the highest levels of diversity were observed in wild rabbits from Portugal, with wild rabbits from England and domestic rabbits showing less diversity. Within the sample, several recombination events were detected and trans-specific evolution of alleles was evidenced, both being general characteristics of mammalian MHC genes. Positive selection is implicated as operating on six codons within exon 2, which are also subject to positive selection in other mammals. Some of these positions are putative antigen recognition sites and underline the importance of pathogen-driven selection on these MHC genes.

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The allotypic patchwork pattern of the rabbit IGKC1 allele b5wf : genic exchange or common ancestry?

et al. 1999

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The protein sequences of different alleles of the rabbit immunoglobulin IGKC1 gene can differ at more than 40% of the amino acid positions. This exceptional degree of allelic divergence raises questions concerning the causal underlying mechanisms. We report the DNA sequence of the coding region of an allotype which is associated with the mitochondrial lineage A (Southwestern Spain). At the serological level, this b5wf allotype presents a patchwork of antigenic determinants which in domestic breeds are characteristic of the b4, b5, and b6 allotypes. The inferred protein sequence of the b5wf allotype was found to differ from that of the b4, b5, and b6 allotypes at 25, 10, and 15% of the amino acid positions, respectively. Sequence comparisons show that the b4-specific epitopes of the b5wf allotype are probably due to a shared ThrThrGlnThr motif at Kabat positions 153-156. Similarly, the shared b5-specific determinants should relate to the motifs 161ThrSerLys163 and/or 182LysSerAspGlu185. A monoclonal antibody binding epitope shared among the b5wf, b5, and b6 sequences appeared to be correlated with the presence of Asp190. Although there is evidence of interallelic genic exchange, sequence comparisons suggest that the apparent mosaic structure of the b5wf allotype is better explained by common ancestry and point mutation.

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Characterization and DNA sequence of the b6w2 allotype of the rabbit immunoglobulin kappa 1 light chain (b locus)

Cited by 7 publications

References 38 publications

Cytonuclear disequilibria in wild populations of rabbit ( Oryctolagus cuniculus L. ) suggest unequal allele turnover rates at the b locus ( IGKC1 )

Cytonuclear disequilibria in wild populations of rabbit ( Oryctolagus cuniculus L. ) suggest unequal allele turnover rates at the b locus ( IGKC1 )

Diversity and evolutionary history of the MHC DQA gene in leporids

The allotypic patchwork pattern of the rabbit IGKC1 allele b5wf : genic exchange or common ancestry?

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