Characterization and distribution of the hemagglutinins produced by Vibrio cholerae

Abstract

Examination of the distribution of cell-associated and soluble hemagglutinins (HA) produced by Vibrio cholerae revealed the existence offour different HAs. A cell-associated mannose-sensitive HA (MSHA) was produced only by the El Tor biotype. This was evident with all El Tor strains examined. It appears to be responsible for the HA biotyping differentiation of El Tor from classical biotype V. cholerae. The MSHA had no apparent divalent ion requirement; it was inhibited by D-mannose and D-fructose; and it was active on all human (A, B, 0) and all chicken erythrocytes tested. Spontaneous MSHAmutants of El Tor strains were selected by cosedimentation of MSHA+ parent bacteria with erythrocytes. An L-fucose-sensitive HA was detected transiently in early logphase growth with two of the four classical strains examined and with MSHAmutants of El Tor biotype strains 3083, 26-3, and 17. MSHAmutants also expressed another cell-associated HA in late log-phase cultures. A "soluble" HA was detected in late log-phase cultures of all strains tested. This HA was not inhibitable by any sugars tested; it required CA2+ ions for maximum activity; and it was active on some chicken erythrocytes but not others.

Spatiotemporal activity of the mshA gene system in Shewanella oneidensis MR-1 biofilms

“…Inhibition of pili-mediated cellular agglutination and surface adhesion by the hexose D-mannose has been reported, and has been used to characterize the initial steps in biofilm formation by Vibrio cholerae and E. coli (Bhattacharjee & Srivastava, 1978;Hanne & Finkelstein, 1982;Pratt & Kolter, 1998;Moorthy & Watnick, 2004). In order to test the molecular properties of the MSHA pili in S. oneidensis, we explored whether biofilms are sensitive to carbohydrate addition, and developed an assay to probe whether the stability of established biofilms is dependent on MSHA-mediated cellular adhesion.…”

Hemagglutinating activity of vibrio cholerae enterotoxin

“…cholerae is known to produce various hemagglutinins (lectins) [3][4][5][6]. These differ immunologically and biologically from cholera toxin, which is an enterotoxin produced by V. cholerae [7][8][9]. One of these lectins has been well characterized and shown to be a mannose-specific lectin [7][8][9].…”

“…These differ immunologically and biologically from cholera toxin, which is an enterotoxin produced by V. cholerae [7][8][9]. One of these lectins has been well characterized and shown to be a mannose-specific lectin [7][8][9]. It is supposed to play important roles in attachment of V. cholerae to epithelial membranes.…”

“…Vibrio cholerae has been shown to produce a variety of lectins [3][4][5][6]. One of these lectins has been shown to be a cell-associated mannose-specific lectin, produced by the E1 Tor biotype [7], which was also inhibited by fructose. This lectin has been shown to be a polymeric protein with a proteolytic activity which appears to participate in the attachment of V. cholerae to intestinal epithefium [8,9].…”

Adhesion of Vibrio cholerae 01 to human buccal epithelial cells in vitro

“…Haemagglutination is an easily observable manifestation of the adhesive properties of bacteria. Previous reports [10] have described mannose-sensitive haemagglutinin in V. cholerae 01 biotype el tor and suggested that it may mediate attachment to host epithelial cell surfaces. In the present study, D-mannose could inhibit the adhesion only weakly, suggesting that the microbial and host factors that govern the adhesion of vibrios are complex.…”