Characterization and Distribution of Selenite Reduction Products in Cultures of the Marine Yeast<i>Rhodotorula mucilaginosa-</i>13B

Ruocco, Maria H. W.; Chan, Clara S.; Hanson, Thomas E.; Church, Thomas M.

doi:10.1080/01490451.2014.888909

Cited by 15 publications

(7 citation statements)

References 49 publications

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“…Colonies of the polymorphic fungus Aureobasidium pullulans weredepending on the SeO 2− 3 concentrationlight pink in the presence of 1 mM selenite but red at a higher (5 mM) concentration (Gharieb et al, 1995). The marine yeast strain Rhodotorula mucilaginosa-13B provides another clear example of the transformation of selenite into elemental Se in the form of intracellular or extracellular Se 0 nanoparticles (Ruocco et al, 2014).…”

Section: Yeastsmentioning

confidence: 99%

Microbial reduction of selenium oxyanions: energy-yielding and detoxification reactions

Lampis¹,

Vallini²

2021

Environmental Technologies to Treat Selenium Pollution

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Selenium (Se), a semi-metallic chemical element in the oxygen group (group 16 [VIa]) of the periodic table, can be beneficialeven essential in some instancesfor microbes and animals, including humans, when present at a suitable concentration, whereas no essential Se requirement has been shown for higher plants (Lenz & Lens, 2009;Winkel et al., 2015). Se is an essential trace element required for the biosynthesis of seleno-amino acids such as selenocysteine (Se-Cys) (Bock et al., 1991;Gromer et al., 2005) and selenomethionine (Se-Met, the major dietary form) (Schrauzer, 2000). These are potent antioxidants as well as a source of Se for the synthesis of Se-dependent antioxidant and repair proteins such as glutathione peroxidases, thioredoxin reductases, and methionine sulfoxide reductases (

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Section: Yeastsmentioning

confidence: 99%

Microbial reduction of selenium oxyanions: energy-yielding and detoxification reactions

Lampis¹,

Vallini²

2021

Environmental Technologies to Treat Selenium Pollution

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“…The study made by Ruocco et al ( 2014 ) showed that Rhodotorula mucilaginosa -13B yeasts have the ability to reduce selenium in its structures. Microscopic observations have confirmed the presence of elemental selenium deposited in yeast cells.…”

Section: Intracellular Accumulation Of Seleniummentioning

confidence: 99%

Accumulation and metabolism of selenium by yeast cells

Kieliszek

Błażejak

Gientka

et al. 2015

Appl Microbiol Biotechnol

169

130

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This paper examines the process of selenium bioaccumulation and selenium metabolism in yeast cells. Yeast cells can bind elements in ionic from the environment and permanently integrate them into their cellular structure. Up to now, Saccharomyces cerevisiae, Candida utilis, and Yarrowia lipolytica yeasts have been used primarily in biotechnological studies to evaluate binding of minerals. Yeast cells are able to bind selenium in the form of both organic and inorganic compounds. The process of bioaccumulation of selenium by microorganisms occurs through two mechanisms: extracellular binding by ligands of membrane assembly and intracellular accumulation associated with the transport of ions across the cytoplasmic membrane into the cell interior. During intracellular metabolism of selenium, oxidation, reduction, methylation, and selenoprotein synthesis processes are involved, as exemplified by detoxification processes that allow yeasts to survive under culture conditions involving the elevated selenium concentrations which were observed. Selenium yeasts represent probably the best absorbed form of this element. In turn, in terms of wide application, the inclusion of yeast with accumulated selenium may aid in lessening selenium deficiency in a diet.

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“…Other authors [56] determined that in R. glutinis X-20, selenium accumulation is highly dependent on pH; in particular, pH 7 favors selenium accumulation in this strain, unlike what has been established in other studies where selenium accumulation was reported to be favorable at an acidic pH [57] Thus, strains of C. utilis Yarrowia lipolytica have been used increasingly in biotechnological studies with promising results, with yields of up to 3000 µg Se/g of dry yeast [58]. Other microorganisms have also been reported with the ability to incorporate inorganic selenium into their cell structures, such as filamentous fungi [59], bacteria of the genus Enterobacter [60], and red yeasts like R. mucilaginosa-13B [21]. Studies have been conducted on the optimization of organic selenium bioaccumulation, considering different concentrations of NaSe in the culture medium, initial pH variation, different times of inoculation of inorganic selenium, and cultivation time [43].…”

Section: Discussionmentioning

confidence: 99%

“…[ 11 ], Kluyveromyces spp., and Rhodotorula spp. [ 19 , 20 , 21 ], in which the capacity for selenium accumulation in their cellular structure has also been demonstrated [ 22 , 23 ]. In recent years, SY supplementation has also been reported to enhance growth, feed conversion, and immunocompetence in channel catfish ( Ictalurus punctatus ), hybrid striped bass ( Moronechrysops Morone saxatilis ), rainbow trout ( Oncorhynchus mykiss ), and marron ( Cherax cainii ) [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development

Díaz-Navarrete,

Sáez-Arteaga,

Marileo

et al. 2024

Biomolecules

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It is known that selenium (Se) is an essential trace element, important for the growth and other biological functions of fish. One of its most important functions is to contribute to the preservation of certain biological components, such as DNA, proteins, and lipids, providing protection against free radicals resulting from normal metabolism. The objective of this study was to evaluate and optimize selenium accumulation in the native yeast Rhodotorula mucilaginosa 6S. Sodium selenite was evaluated at different concentrations (5–10–15–20–30–40 mg/L). Similarly, the effects of different concentrations of nitrogen sources and pH on cell growth and selenium accumulation in the yeast were analyzed. Subsequently, the best cultivation conditions were scaled up to a 2 L reactor with constant aeration, and the proteome of the yeast cultured with and without sodium selenite was evaluated. The optimal conditions for biomass generation and selenium accumulation were found with ammonium chloride and pH 5.5. Incorporating sodium selenite (30 mg/L) during the exponential phase in the bioreactor after 72 h of cultivation resulted in 10 g/L of biomass, with 0.25 mg total Se/g biomass, composed of 25% proteins, 15% lipids, and 0.850 mg total carotenoids/g biomass. The analysis of the proteomes associated with yeast cultivation with and without selenium revealed a total of 1871 proteins. The results obtained showed that the dynamic changes in the proteome, in response to selenium in the experimental medium, are directly related to catalytic activity and oxidoreductase activity in the yeast. R. mucilaginosa 6S could be an alternative for the generation of selenium-rich biomass with a composition of other nutritional compounds also of interest in aquaculture, such as proteins, lipids, and pigments.

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Characterization and Distribution of Selenite Reduction Products in Cultures of the Marine YeastRhodotorula mucilaginosa-13B

Cited by 15 publications

References 49 publications

Microbial reduction of selenium oxyanions: energy-yielding and detoxification reactions

Microbial reduction of selenium oxyanions: energy-yielding and detoxification reactions

Accumulation and metabolism of selenium by yeast cells

Enhancing Selenium Accumulation in Rhodotorula mucilaginosa Strain 6S Using a Proteomic Approach for Aquafeed Development

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