Characterization and distribution of FMRFamide immunoreactivity in the rat central nervous system

O’Donohue, Thomas L.; Bishop, John F.; Chronwall, Bibie M.; Groome, James R.; Watson, Winsor H.

doi:10.1016/0196-9781(84)90087-1

Cited by 131 publications

(34 citation statements)

References 34 publications

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“…The chemical structural resemblance (ArgPhe-amide) between FMRFa-like peptides and g-MSHs, their comparable haemodynamic pro®le (increase in MAP and HR) (Allard et al, 1995;Barnard & Dockray, 1984a;De Wildt et al, 1993;Mues et al, 1982;Thiemermann et al, 1991;Wong et al, 1985) and correspondent anatomical location of these peptides and their receptors within the brain (hypothalamus and nucleus tractus solitarius) (Fodor et al, 1996;Kivipelto et al, 1989;Majane et al, 1989;O'Donohue et al, 1984;Pittius et al, 1984), makes the FMRFa/NPFFa/MERFa receptor a very important candidate for mediating the cardiovascular e ects of g-MSHs. Therefore, in the present study various peptides with a Cterminal Arg-Phe structure are tested for their haemodynamic pro®le in conscious rats.…”

Section: Introductionmentioning

confidence: 99%

Relevance of the C‐terminal Arg‐Phe sequence in γ₂‐melanocyte‐stimulating hormone (γ₂‐MSH) for inducing cardiovascular effects in conscious rats

Nijsen

Ruiter

Kasbergen

et al. 2000

British J Pharmacology

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1 The cardiovascular e ects by g 2 -melanocyte-stimulating hormone (g 2 -MSH) are probably not due to any of the well-known melanocortin subtype receptors. We hypothesize that the receptor for PheMet-Arg-Phe-amide (FMRFa) or Phe-Leu-Phe-Gln-Pro-Gln-Arg-Phe-amide (neuropeptide FF; NPFFa), other Arg-Phe containing peptides, is the candidate receptor. Therefore, we studied various Arg-Phe containing peptides to compare their haemodynamic pro®le with that of g 2 -MSH(6 ± 12), the most potent fragment of g 2 -MSH. 2 Mean arterial pressure (MAP) and heart rate (HR) changes were measured in conscious rats after intravenous administration of g 2 -MSH related peptides. 3 Phe-Arg-Trp-Asp-Arg-Phe-Gly (g 2 -MSH(6 ± 12)), FMRFa, NPFFa, Met-enkephalin-Arg-Pheamide (MERFa), Arg-Phe-amide (RFa), acetyl-Phe-norLeu-Arg-Phe-amide (acFnLRFa) and desamino-Tyr-Phe-norLeu-Arg-Phe-amide (daYFnLRFa) caused a dose-dependent increase in MAP and HR. g 2 -MSH(6 ± 12) showed the most potent cardiovascular e ects (ED 50 =12 nmol kg 71 for DMAP; 7 nmol kg 71 for DHR), as compared to the other Arg-Phe containing peptides (ED 50 =177 ± 292 nmol kg 71 for DMAP; 130 ± 260 nmol kg 71 for DHR). 4 Peptides, which lack the C-terminal Arg-Phe sequence (Lys-Tyr-Val-Met-Gly-His-Phe-Arg-TrpAsp-Arg-Pro-Gly (g 2 -pro 11 -MSH), desamino-Tyr-Phe-norLeu-Arg-[L-1,2,3,4 tetrahydroisoquinoline-3-carboxylic acid]-amide (daYFnLR[TIC]a) and Met-enkephalin (ME)), were devoid of cardiovascular actions. 5 The results indicate that the baroreceptor re¯ex-mediated reduction of tonic sympathetic activity due to pressor e ects is inhibited by g 2 -MSH(6 ± 12) and that its cardiovascular e ects are dependent on the presence of a C-terminal Arg-Phe sequence. 6 It is suggested that the FMRFa/NPFFa receptor is the likely candidate receptor, involved in these cardiovascular e ects.

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Section: Introductionmentioning

confidence: 99%

Relevance of the C‐terminal Arg‐Phe sequence in γ₂‐melanocyte‐stimulating hormone (γ₂‐MSH) for inducing cardiovascular effects in conscious rats

Nijsen

Ruiter

Kasbergen

et al. 2000

British J Pharmacology

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“…It recognizes the RFamide terminal and should, therefore, recognize tissue peptides belonging to the FMRFamide family. Nevertheless, it has previously been observed that antibodies against FaRPs can crossreact with some members of the pancreatic polypeptide family, in particular with NPY Hokfelt et al, 1983;O'Donohue et al, 1984;Triepel and Grimmelikhuijzen, 1984a;Majane and Yang, 1987;Muske and Moore, 1987;Bonn and Konig, 1988;Chen et al, 1989;Kivipelto et al, 1989;Shen and Cheng, 1989;Vallarino et al, 1991Vallarino et al, , 1993Fujii and Kobayashi, 1992a;Boersma et al, 1993;Fischer et al, 1996;Yamamoto et al, 1996]. In this study, we consistently observed that preabsorption of anti-FMRFamide (diluted 1:10,000) with 5 µM of synthetic NPY totally eliminated the immunostaining in the dorsal cortex and preoptic nucleus, and the intensity of immunoreaction in other brain nuclei was reduced.…”

Section: Technical Considerationsmentioning

confidence: 99%

Localization of FMRFamide-Like Immunoreactivity in the Brain of the Viviparous Skink <i>(Chalcides chalcides)</i>

D’Aniello¹,

Fiorentin²,

Pinelli³

et al. 2001

Brain Behav Evol

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Neuroanatomical distribution of FMRFamide-like immunoreactivity was investigated in the brain and olfactory system of the viviparous skink, Chalcides chalcides. In the adult brain FMRFamide immunoreactive (ir) perikarya were observed in the diagonal band of Broca, medial septal nucleus, accumbens nucleus, bed nucleus of the anterior commissure, periventricular hypothalamic nucleus, lateral forebrain bundle, and lateral preoptic, subcommissural, suprachiasmatic and lateral hypothalamic areas. This pattern was seen in both male and female brains. Though all major brain areas showed FMRFamide-ir innervation, the densest ir fiber network was observed in the hypothalamus. During development, ir elements were observed for the first time in embryos at mid-pregnancy. FMRFamide perikarya were located along the ventral surface of the vomeronasal nerve, in the olfactory peduncle mediobasally, as well as in the anterior olfactory nucleus and olfactory tubercle. Furthermore, some ir neurons were observed in the rhombencephalic reticular substance; however, the ir fiber network was poorly developed. Later in development FMRFamide-ir neurons appeared also in the bed nucleus of the anterior commissure as well as the rhombencephalic nucleus of solitary tract and the dorsal motor nucleus of vagus nerve. In juveniles, the distribution profile of FMRFamide immunoreactivity was substantially similar to that of the adults, with a less widespread neuronal distribution and a more developed fiber network. Ontogenetic presence of FMRFamide immunoreactivity in the nasal area has been linked to the presence of a nervus terminalis in this reptile.

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“…We used a rabbit polyclonal antiserum anti-mouse IgG. Characterization of the antiserum to FMRFamide used in this study showed it to be highly specific for the COOH-terminal amino acid sequence, -Arg-Phe-NH2 (22). A characterization of the monoclonal antibody to SCPB used in this study is forthcoming (B. Masinovsky, S. Kempf, J. Calloway, and A.…”

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Peptidergic modulation of patterned motor activity in identified neurons of Helisoma

Murphy¹,

Lukowiak²,

Stell

1985

Proc. Natl. Acad. Sci. U.S.A.

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The neuroaftive peptides SCPB (small cardioactive peptide B) and FMRFamide (Phe-Met-Arg-Phe-NH2), both originally isolated from molluscs, have potent modulatory effects upon the production of patterned motor activity in identified neurons (e.g., B5 and B19) in the buccal ganglia of the snail Helisoma. Such patterned motor activity has previously been shown to underlie feeding behavior. (7,8).Several aspects of the neural basis of feeding in the snail Helisoma trivolvis have been described. Cyclical activity of many identified effector neurons and elements of the central pattern generator has been characterized (9-11). In addition, the monoamines serotonin (12) and dopamine (13) have been shown to activate or modulate such patterned motor activity (PMA) associated with feeding and generated by neurons in the buccal ganglia. As a first step in characterizing the potential roles of neuroactive peptides in regulating feeding behavior in Helisoma, we report here that two neuroactive peptides, originally discovered in molluscs, have profound effects on the production of PMA (which apparently represents "fictive feeding") by buccal ganglion neurons. Small cardioactive peptide B (SCPB, Met-Asn-Tyr-Leu-Ala-PhePro-Arg-Met-NH2; ref. 14) has stimulatory effects upon the generation of PMA that resemble, at least superficially, those described for serotonin (5-hydroxytryptamine) and dopamine. In contrast, FMRFamide (Phe-Met-Arg-Phe-NH2; ref.15) inhibits and, at a sufficient concentration (10-5 M), effectively suppresses PMA in the buccal ganglia. SCP has been indicated, by chromatographic techniques, to occur in Helisoma central nervous system (see table 1 in ref. 14). We show here that the buccal ganglion ofHelisoma contains both neurons with SCPB-like immunoreactivity and neurons with anti-FMRFamide immunoreactivity.MATERIALS AND METHODS Albino specimens of Helisoma trivolvis were used; they were obtained from stocks maintained in the laboratory of A. G. M. Bulloch (University of Calgary) and derived from the Oregon Red stock of S. B. Kater's laboratory (University of Iowa). The dissection, the physiological saline, and the electrophysiological recording and display techniques have been described (16,17). For these experiments, either isolated buccal ganglia or buccal ganglia attached to the central ring of ganglia via the cerebrobuccal connectives were used. There was no detectable difference in the effects of peptides upon PMA whether or not the central ganglia were attached, but the rate of spontaneous PMA varies considerably and tends to be higher in isolated buccal ganglia. Once it had been determined that SCPB was excitatory and FMRFamide was inhibitory, relatively quiescent preparations were used for SCPB experiments and relatively active preparations were used for FMRFamide experiments. The synthetic peptides, SCPB (Peninsula Laboratories, San Carlos, CA) and FMRFamide (Sigma), were used. Saline volume (<0.5 ml) in the recording chamber was maintained by suction from the surface, and solutions were changed ...

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Characterization and distribution of FMRFamide immunoreactivity in the rat central nervous system

Cited by 131 publications

References 34 publications

Relevance of the C‐terminal Arg‐Phe sequence in γ₂‐melanocyte‐stimulating hormone (γ₂‐MSH) for inducing cardiovascular effects in conscious rats

Relevance of the C‐terminal Arg‐Phe sequence in γ₂‐melanocyte‐stimulating hormone (γ₂‐MSH) for inducing cardiovascular effects in conscious rats

Localization of FMRFamide-Like Immunoreactivity in the Brain of the Viviparous Skink <i>(Chalcides chalcides)</i>

Peptidergic modulation of patterned motor activity in identified neurons of Helisoma

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Characterization and distribution of FMRFamide immunoreactivity in the rat central nervous system

Cited by 131 publications

References 34 publications

Relevance of the C‐terminal Arg‐Phe sequence in γ2‐melanocyte‐stimulating hormone (γ2‐MSH) for inducing cardiovascular effects in conscious rats

Relevance of the C‐terminal Arg‐Phe sequence in γ2‐melanocyte‐stimulating hormone (γ2‐MSH) for inducing cardiovascular effects in conscious rats

Localization of FMRFamide-Like Immunoreactivity in the Brain of the Viviparous Skink <i>(Chalcides chalcides)</i>

Peptidergic modulation of patterned motor activity in identified neurons of Helisoma

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Relevance of the C‐terminal Arg‐Phe sequence in γ₂‐melanocyte‐stimulating hormone (γ₂‐MSH) for inducing cardiovascular effects in conscious rats

Relevance of the C‐terminal Arg‐Phe sequence in γ₂‐melanocyte‐stimulating hormone (γ₂‐MSH) for inducing cardiovascular effects in conscious rats