Characterization and discrimination of microbial community and co‐occurrence patterns in fresh and strong flavor style flue‐cured tobacco leaves

Zhang, Qianying; Zhang, Geng; Li, Dongliang; Ding, Zhongyang

doi:10.1002/mbo3.965

Cited by 34 publications

(28 citation statements)

References 62 publications

(84 reference statements)

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“…To our knowledge, here we described the first survey of Symmetrospora, representing yeasts occurring on tomato leaf surfaces. These red basidiomycete yeasts, where previously phylogenetically described as Sporobolomycetes and have been found on tobacco [45,104]. Their role in microbial communities remains unknown.…”

Section: Phyllosphere Microbiome Impacted By Tomato Species Across Ge...mentioning

confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

et al. 2022

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness, and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops, and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops; however, little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum, and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over 2 consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi, and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa, and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner. Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves.Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype, and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

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Section: Phyllosphere Microbiome Impacted By Tomato Species Across Ge...mentioning

confidence: 99%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

et al. 2022

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“…Owing to concerns about environmental contamination and harmful effects on nontarget organisms, the demand for ecofriendly alternatives to chemical control has increased. Therefore, due to the widespread and refractory nature of the disease, no matter what fluecured tobacco and storage technology is used, an alternative control of this disease is a serious challenge (Li et al, 2019;Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Bacillus amyloliquefaciens B9601-Y2 as a Potential Antagonist of Tobacco Leaf Mildew Pathogen During Flue-Curing

Pan

Munir

et al. 2021

Front. Microbiol.

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Tobacco leaf mildew caused by Rhizopus oryzae (Mucorales, Zygomycota) is an important and devastating post-harvest disease during flue-cured tobacco period, and also is known to cause diseases of fruits and vegetables. In this study, assessment of several candidate biological control agents were first tested in vitro to determine their antifungal activities and potential strains were further applied to tobacco leaves to prevent pathogen colonization during the tobacco-curing process. In vitro screening of 36 bacteria and the isolates of one fungus were performed for their antifungal activities against R. oryzae using dual culture method. Potential five isolates viz. Bacillus amyloliquefaciens B9601-Y2 (Y2), B. amyloliquefaciens YN201728 (YN28), Pseudomonas sp. (Pb), and B. amyloliquefaciens YN201732 (YN32) and T. harzianum B (Th-B) from total screened isolates have shown remarkable results for controlling the mycelial growth of R. oryzae. Finally, out of these five isolates, B. amyloliquefaciens B9601-Y2 potentially reduced the mycelial growth of fungal pathogen with great inhibitory effect. In order to get a better understanding of the biocontrol effect of B9601-Y2 in a flue-curing barn, various suspension density tests with two application methods involving spraying and soaking were examined. Two application methods of B. amyloliquefaciens B9601-Y2 had 98.60 and 98.15% control effects, respectively. In curing barn, the incidence in the treatment group was significantly reduced and tobacco leaves did not get mildew. Altogether, the study demonstrated that candidate bacterial strain B. amyloliquefaciens B9601-Y2 is a potential antagonist for the management of tobacco leaf mildew during flue-curing.

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“…These red basidiomycete yeasts, where previously phylogenetically described as Sporobolomycetes and have been found on tobacco (Q. Zhang et al 2020;Haelewaters et al 2020).…”

Section: Discussionmentioning

confidence: 95%

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

Runge

Ventura

Kemen

et al. 2021

Preprint

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Plants are colonized by myriads of microbes across kingdoms, which affect host development, fitness and reproduction. Hence, plant microbiomes have been explored across a broad range of host species, including model organisms, crops and trees under controlled and natural conditions. Tomato is one of the world’s most important vegetable crops, however little is known about the microbiota of wild tomato species. To obtain insights into the tomato microbiota occurring in natural environments, we sampled epiphytic microbes from leaves of four tomato species, Solanum habrochaites, S. corneliomulleri, S. peruvianum and S. pimpinellifolium, from two geographical locations within the Lima region of Peru over two consecutive years. Here, a high-throughput sequencing approach was applied to investigate microbial compositions including bacteria, fungi and eukaryotes across tomato species and geographical locations. The phyllosphere microbiome composition varies between hosts and location. Yet, we identified persistent microbes across tomato species that form the tomato microbial core community. In addition, we phenotypically defined healthy and dysbiotic samples and performed a downstream analysis to reveal the impact on microbial community structures. To do so, we compared microbial diversities, unique OTUs, relative abundances of core taxa and microbial hub taxa, as well as co-occurrence network characteristics in healthy and dysbiotic tomato leaves and found that dysbiosis affects the phyllosphere microbial composition in a host species-dependent manner Yet, overall, the present data suggests an enrichment of plant-promoting microbial taxa in healthy leaves, whereas numerous microbial taxa containing plant pathogens occurred in dysbiotic leaves. Concluding, we identify the core phyllosphere microbiome of wild tomato species, and show that the overall phyllosphere microbiome can be impacted by sampling time point, geographical location, host genotype and plant health. Future studies in these components will help understand the microbial contribution to plant health in natural systems and can be of use in cultivated tomatoes.

show abstract

Characterization and discrimination of microbial community and co‐occurrence patterns in fresh and strong flavor style flue‐cured tobacco leaves

Cited by 34 publications

References 62 publications

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru upon Dysbiosis

Deciphering the Bacillus amyloliquefaciens B9601-Y2 as a Potential Antagonist of Tobacco Leaf Mildew Pathogen During Flue-Curing

Distinct Phyllosphere Microbiome of Wild Tomato Species in Central Peru Upon Dysbiosis

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