Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis

Colniță, Alia; Dina, Nicoleta Elena; Leopold, Nicolae; Vodnar, Dan Cristian; Bogdan, Diana; Porav, Sebastian; David, L.

doi:10.3390/nano7090248

Cited by 36 publications

(21 citation statements)

References 73 publications

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“…The large enhancement of this vibrational mode indicates that adsorption of AMP onto the Ag surface is through the purine moiety. Similar results were obtained using this methodology to obtain SERS spectra of Gram-positive bacteria [ 42 ].…”

Section: Resultssupporting

confidence: 75%

Review on SERS of Bacteria

Mosier‐Boss¹

2017

Biosensors

104

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Surface enhanced Raman spectroscopy (SERS) has been widely used for chemical detection. Moreover, the inherent richness of the spectral data has made SERS attractive for use in detecting biological materials, including bacteria. This review discusses methods that have been used to obtain SERS spectra of bacteria. The kinds of SERS substrates employed to obtain SERS spectra are discussed as well as how bacteria interact with silver and gold nanoparticles. The roll of capping agents on Ag/Au NPs in obtaining SERS spectra is examined as well as the interpretation of the spectral data.

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Section: Resultssupporting

confidence: 75%

Review on SERS of Bacteria

Mosier‐Boss¹

2017

Biosensors

104

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“…It is not surprising that the SERS spectrum of bacteria exhibits vibrational bands which are also common in the spectra of biological tissues and cells [25]. As depicted in Figure 6 the new band obtained 1050 cm −1 can be attributed to the vibrations of some sugars or amino sugars located in the membrane [26]. This finding can be explained by the fact that the cell membranes contain various carbohydrates.…”

Section: Resultsmentioning

confidence: 99%

Understanding Escherichia coli damages after chlorophyllin‐based photosensitization

Zudyte

Velička

Šablinskas

et al. 2020

Journal of Biophotonics

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Pathogenic strains of bacteria are causing various illnesses all around the world and have a major socioeconomic impact. Thus, fast-and low-cost methods for the microbial control of foods are needed. One of them might be photosensitization. This study looks deeper into the mechanism of Escherichia coli damage by chlorophyllin-based photosensitization. Fluorimetric data indicate that after 15 minute incubation with chlorophyllin (Chl) (1.5 × 10 −5 M Chl) 0.73 ± 0.03 μM of this compound was associated with E. coli cell surface. After photoactivation (405 nm, 6-30 J/cm 2) significant reduction (88.2%) of bacterial viability was observed. Higher concentration of Chl (5 × 10 −4 M Chl) reduced viability of bacteria more than by 98%. Results indicated that reactive oxygen species (ROS) took place in this inactivation. Colloidal surface enhanced Raman scattering (SERS) spectroscopy was employed to detect the molecular changes in the treated bacteria. It was found that Chl-based based photosensitization triggers multiple surface structure changes in E. coli what induce lethal unrepairable damages and inactivation of pathogen.

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“…For example, Lactobacillus casei and Listeria monocytogenes were successfully discriminated by applying them to their specific spectral data. [121] Avci et al analyzed the spectral changes of seven different UTI-related bacteria at different growth stages, and the sources of spectral bands were described in detail. The results showed that regardless of the growth stage of the bacteria, all species could be distinguished using this PCA method.…”

Section: Data Processing Methods Combined With Sers Bacterial Detectionmentioning

confidence: 99%

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

et al. 2020

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The rapid, highly sensitive, and accurate detection of bacteria is the focus of various fields, especially food safety and public health. Surface-enhanced Raman spectroscopy (SERS), with the advantages of being fast, sensitive, and nondestructive, can be used to directly obtain molecular fingerprint information, as well as for the on-line qualitative analysis of multicomponent samples. It has therefore become an effective technique for bacterial detection. Within this progress report, advances in the detection of bacteria using SERS and other compatible techniques are discussed in order to summarize its development in recent years. First, the enhancement principle and mechanism of SERS technology are briefly overviewed. The second part is devoted to a label-free strategy for the detection of bacterial cells and bacterial metabolites. In this section, important considerations that must be made to improve bacterial SERS signals are discussed. Then, the label-based SERS strategy involves the design strategy of SERS tags, the immunomagnetic separation of SERS tags, and the capture of bacteria from solution and dye-labeled SERS primers. In the third part, several novel SERS compatible technologies and applications in clinical and food safety are introduced. In the final part, the results achieved are summarized and future perspectives are proposed.

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Characterization and Discrimination of Gram-Positive Bacteria Using Raman Spectroscopy with the Aid of Principal Component Analysis

Cited by 36 publications

References 73 publications

Review on SERS of Bacteria

Review on SERS of Bacteria

Understanding Escherichia coli damages after chlorophyllin‐based photosensitization

Bacteria Detection: From Powerful SERS to Its Advanced Compatible Techniques

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