Characterization and determination of trace alkaloids in seeds extracts from<i>Peganum harmala</i>linn. Using LC-ESI-MS and HPLC

Liu, L.; Zhao, Ting; Cheng, Xuemei; Wang, C. H.; Wang, Z. T.

doi:10.1556/achrom.25.2013.2.2

Cited by 27 publications

(12 citation statements)

References 19 publications

(20 reference statements)

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“…The characteristic fragment ions were key to study and understand the fragmentation mechanisms and to support metabolite annotation with a higher confidence level (Figures 2 and 3). Besides they are in agreement with previous studies [22][23][24]. Results show that P. viridis, M. hostilis and Dc Ab are the ones that contain the highest levels of tryptamine alkaloids, mainly DMT and 5-OH-DMT [25].…”

Section: Characterization Of Alkaloid Derivatives and Other Compoundssupporting

confidence: 92%

Evaluation of the Cytotoxicity of Ayahuasca Beverages

et al. 2020

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Ayahuasca is a beverage consumed at shamanic ceremonies and currently has gained popularity on recreational scenarios. It contains beta-carboline alkaloids and N,N-dimethyltryptamine, which possesses hallucinogenic effects. Only a few studies have elicited the psychoactive effects and the dose of such compounds on neurological dopaminergic cells or animals. In this work, we aimed to study the cytotoxic effects of these compounds present in ayahuasca beverages and on five different teas (Banisteriopsis caapi, Psychotria viridis, Peganum harmala, Mimosa tenuiflora and Dc Ab (commercial name)) preparations on dopaminergic immortalized cell lines. Moreover, a characterization of the derivative alkaloids was also performed. All the extracts were characterized by chromatographic systems and the effect of those compounds in cell viability and total protein levels were analyzed in N27 dopaminergic neurons cell line. This is the first article where cytotoxicity of ayahuasca tea is studied on neurological dopaminergic cells. Overall, results showed that both cell viability and protein contents decreased when cells were exposed to the individual compounds, as well as to the teas and to the two mixtures based on the traditional ayahuasca beverages.

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Section: Characterization Of Alkaloid Derivatives and Other Compoundssupporting

confidence: 92%

Evaluation of the Cytotoxicity of Ayahuasca Beverages

et al. 2020

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“…The resulting collapse of the proton motrice force and depletion of the ATP pool lead to eventual cell death. Liu et al (2013) determined the main and trace alkaloids in the seed extracts of P. harmala using liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) and high-performance liquid chromatography (HPLC).They found that Harmaline and harmine make more than 70% of the alkaloids. Diglycoside vasicine, vasicine, vasicinone, harmalol, harmol, tetrahydroharmine, 8-hydroxy-harmine, ruine, harmaline, and harmine were found to be the other alkaloids.…”

Section: Discussionmentioning

confidence: 99%

In vitro ovicidal activity of Peganum harmala seeds extract on the eggs of Fasciola hepatica

et al. 2016

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seeds extract has been previously reported to have antimicrobial and other medicinal properties. The aim of this study was to evaluate the ovicidal activity of the methanolic extract of seeds against the eggs of The phenolic compounds of the methanolic extract of seeds were identified by HPLC analysis. Catechin, rutin, p-Coumaric acid, chloregenic acid and hesperetin were found to be the major phenolic compounds. eggs were collected from the gall bladder of naturally infected sheep. The eggs were exposed to two concentrations of seeds extract (1 and 3 mg/mL) for 24 and 48 h. To investigate the effect of the seeds extract on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that eggs were susceptible to the methanolic extract of seeds. Following 24 h exposure of the eggs to seeds extract with concentrations of 1 and 3 mg/mL, the miracidial formation reduced to 5 and 2.2 % respectively (compared with 60 % for the control group). Following 48 h of exposure of the eggs to seeds extract with 1 mg/mL concentration, the miracidial formation reduced to 0.5 %. In this exposure time, no miracidial formation was observed in the eggs exposed to seeds extract with concentration of 3 mg/mL. Therefore, the results of this study indicated that seeds extract has high ovicidal activity against the eggs of . Accordingly, this extract may have the potential flukicidal activity against the immature and mature.

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“…In terms of main active components alkaloids, the seeds of P. harmala are rich in β-carboline alkaloids harmine, harmane, and harmaline which showed to possess hallucinogenic effects (Araújo et al, 2015;Cheng et al, 2010) and are lacking in quinazoline alkaloids, namely, VAS, DVAS, and VAO. By contrast, APP mainly contain the quinazoline alkaloids (VAS, DVAS, and VAO) and contain only trace amounts of β-carboline alkaloids harmine, harmane, and harmaline (Cheng et al, 2010;Liu et al, 2013;Wang et al, 2002;Wen et al, 2014). The fact that APP contains only trace amounts of them, APP appears suitable for the development of an antitussive and expectorant phytomedicine in the future.…”

Section: Discussionmentioning

confidence: 99%